| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Outline of Final Research Achievements |
This study aims to elucidate molecular recognition by "ensemble structure," which cannot be explained by the conventional lock and key model. We first attempted to trap metastable states by forming a complex between an oxidative stress sensor and the organic compound, but we found that cis-prenyltransferase was useful as another model system and determined its X-ray structure. Since it was difficult to obtain the stress sensor crystals of suitable size for X-ray diffraction experiments, we turned our attention to microcrystal electron diffraction using cryo-electron microscopy, which is capable of analyzing even microcrystals. The structural analysis of small molecule samples was first performed by using the molecular replacement method. Since the cryo-EM was installed in our department recently, we have established the basis for future analyses.
|
