Ultra oil cell: Creating a cell factory that mass-produces carotenoids and long chain unsaturated fatty acids
Project/Area Number |
18K19166
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
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Research Institution | The University of Tokyo |
Principal Investigator |
Kawano Shigeyuki 東京大学, フューチャーセンター推進機構, 特任研究員 (70161338)
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Project Period (FY) |
2018-06-29 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 機能性 / 脂肪酸 / クロレラ / 物質生産 / 細胞機能 / カロテノイド / トレボウクシア / 微生物遺伝育種 / 機能性食品 / 生物活性物質 |
Outline of Final Research Achievements |
Using “ultra oil cell” induced by nutrient starvation and intense light stress, we decided to create a cell factory (Chlorella factory) that accumulated functional substances such as ω3 fatty acids and carotenoids. We focused on selecting strains suitable for the cell factory with an awareness of practical application. The phylogenetic relationship of species and strains about the productivity of fats and carotenoids in addition to biomass were clarified by collecting a wide range of green algae, mainly Trebouxiophyceae and conducting molecular phylogenetic analysis. Most of the species and strains investigated can be easily cultured not only with autotrophs but also with heterotrophs and mixed nutrients, and have high biomass productivity. The productivity of fats under heterotrophic nutrition varies greatly depending on the strain, but some strains had an oil content of more than 50%.
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Academic Significance and Societal Importance of the Research Achievements |
2021年4月の「2030年度の温室効果ガス削減目標を2013年度比で46%削減する」との政府発表は、パリ協定にもられた26%でも実現を危ぶまれていたので、各界に衝撃をもって受け止められている。地球温暖化は待ったなしであるが、世代時間の短い微細藻類であれば、10年間の研究期間でも二酸化炭素削減に資する新たな品種を開発できる可能性がある。微細藻類を「育種する」という発想こそが、応用微生物学の新たなトレンドになると考え、多くの株とその培養方法を検討するとともに重イオンビーム照射による分子育種法を開発してきた。カロテノイドと長鎖不飽和脂肪酸を大量生産する「超オイル細胞」の作出はその延長線上にある。
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Sexual difference of optimum environmental condition for growth and maturation of the brown alga Undaria pinnatifida in gametophyte stage2020
Author(s)
Sato, Y., Endo, H., Oikawa, H., Kanematsu, K., Naka, H., Mogamiya, M., Kawano, S., Kazama, Y.
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Journal Title
Genes
Volume: 11
Issue: 8
Pages: 944-944
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The <i>Parachlorella</i> Genome and Transcriptome Endorse Active RWP-RK, Meiosis and Flagellar Genes in Trebouxiophycean Algae2019
Author(s)
Ota, S., Oshima, K., Yamazaki, T., Takeshita, T., Bisova, K., Zachleder, V., Hattori, M. and Kawano, S.
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Journal Title
CYTOLOGIA
Volume: 84
Issue: 4
Pages: 323-330
DOI
NAID
ISSN
0011-4545, 1348-7019
Year and Date
2019-12-25
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Ultrafast simultaneous Raman-fluorescence spectroscopy.2019
Author(s)
Lindley, M., Hiramatsu, K., Nomoto, H., Shibata, F., Takeshita, T., Kawano, S. and Goda, K.
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Journal Title
Anal. Chem.
Volume: 91
Issue: 24
Pages: 15563-15563
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Independent regulation of the lipid and starch synthesis pathways by sulfate metabolites in the green microalga Parachlorella kessleri under sulfur starvation conditions.2018
Author(s)
Yamazaki, T., Konosu, E., Takeshita, T., Hirata, A., Ota, S., Kazama, Y., Abe, T., Kawano, S.
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Journal Title
Algal Res.
Volume: 36
Pages: 37-47
DOI
Related Report
Peer Reviewed
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[Journal Article] Comparison of lipid productivity of Parachlorella kessleri heavy-ion beam irradiation mutant PK4 in laboratory and 150-L mass bioreactor, identification and characterization of its genetic variation.2018
Author(s)
Takeshita, T., Ivanov, I. N., Oshima, K., Ishii, K., Kawamoto, H., Ota, S., Yamazaki, T., Hirata, A., Kazama, Y., Abe, T., Hattori, M., Bisova, K., Zachleder, V. and Kawano, S.
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Journal Title
Algal Res.
Volume: 35
Pages: 416-426
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Carotenoid and lipid accumulation in about twenty species of Chlorophyceae and Trebouxiophyceae under comparatively high light intensity2019
Author(s)
Takeshita, T., Kudo, Y., Hosokawa, S., Erata, M., Kawano, S.
Organizer
AlgalBBB 2019: The 9th International Conference on Algal Biomass, Biofuels and Bioproducts
Related Report
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[Presentation] An optimum condition for promoting growth and maturation at gametophyte stage of Undaria pinnatifida by optimal screening and application for the seedling production on industrial-scale2019
Author(s)
Sato, Y., Oikawa, H., Kanematsu, K., Naka, H., Kawano, S.
Organizer
AlgalBBB 2019: The 9th International Conference on Algal Biomass, Biofuels and Bioproducts
Related Report
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[Patent(Industrial Property Rights)] 抗精神疾患剤2020
Inventor(s)
河野 重行、竹下 毅、礒田 博子、佐々木 一憲
Industrial Property Rights Holder
河野 重行、竹下 毅、礒田 博子、佐々木 一憲
Industrial Property Rights Type
特許
Industrial Property Number
2020-048362
Filing Date
2020
Related Report