Improvement of production efficiency of useful substance by modification of central metabolic pathway
Project/Area Number |
18K19169
|
Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
|
Research Institution | Tokyo Institute of Technology |
Principal Investigator |
Orita Izumi 東京工業大学, 生命理工学院, 助教 (70525964)
|
Project Period (FY) |
2018-06-29 – 2022-03-31
|
Project Status |
Completed (Fiscal Year 2021)
|
Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | メタノール資化性菌 / ポリヒドロキシアルカン酸 / メタノール / メタノー資化性菌 / 代謝改変 |
Outline of Final Research Achievements |
In order to make cell growth compatible with microbial production of value-added compounds on methanol, this study aimed to establish the pathway substitutes for methanol assimilation pathway in the methylotrophic bacterium. Although methanol was assimilated by introduced substitute pathway, restoration of methylotrophic growth was not observed in the cells disrupted central metabolic pathway. On the other hand, reduced growth of the cells in which enzyme catalyzing reaction opposite to central metabolic pathway was introduced, was restored by introducing substitute pathway. This observation might be useful to achieve high production yield of value-added compounds from methanol.
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Academic Significance and Societal Importance of the Research Achievements |
本研究でモデルメタノール資化性菌内に構築した種々のメタノール人工資化経路は、有用物質の収率向上のために今後応用できることが期待され、持続供給可能な材料として注目されているメタノールを原料にした発酵生産に資する微生物の開発に貢献する成果といえる。また、メタボローム解析で得られた知見はメタノール資化性菌の代謝改変をすすめるうえで重要であり、学術的意義も大きい。
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Report
(5 results)
Research Products
(7 results)