| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Viroids are circular single-strand RNA pathogens of about 250-430 nucleotides. SELEX, systematic evolution of ligands by exponential enrichment, was carried out with the aim of selecting aptamers that can specifically bind to viroids. A variety of DNA sequences that bound to full-length potato spindle tuber viroid (PSTVd) RNA were enriched by 15 rounds of SELEX from a random pool of single-stranded 30-nucleotide DNAs. Then based on amplicon analysis of every 5 rounds, 18 species of aptamer candidate sequences that have a potential to bind PSTVd were selected. One of them strongly bound and five weakly bound to the circular form of PSTVd RNA in the binding assay, indicated that DNA aptamers specifically bind to PSTVd were successfully selected.
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