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By manipulating endobacteria, saprophytic Mortierella spp. can be transformed into symbiotic fungi!

Research Project

Project/Area Number 18K19199
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 39:Agricultural and environmental biology and related fields
Research InstitutionIbaraki University

Principal Investigator

Narisawa Kazuhiko  茨城大学, 農学部, 教授 (90431650)

Co-Investigator(Kenkyū-buntansha) 西澤 智康  茨城大学, 農学部, 准教授 (40722111)
Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords内生細菌 / Mortierella属菌 / 植物生育促進 / 共生 / 病原性 / 腐生
Outline of Final Research Achievements

As a result of inoculating 18 isolates of Mortierella sp. harboring a Burkholderiaceae-related endobacteria (BRE) and BRE free isolates on tomato plant, 13 isolates increased the dry weight of the plant as compared to the control. The five isolates among the 13 isolates showed symptoms such as poor root growth and browning leaf. These five isolates that developed the symptoms included one BRE-harboring and 4 BRE-free isolates. From these results, it was suggested that BRE is involved in tomato plant growth promotion.

Academic Significance and Societal Importance of the Research Achievements

本研究では、Mortierella属菌を対象として、同菌に内生する細菌を制御することで腐生菌を共生菌に変える革新的な挑戦を行なった。菌類単独の能力と思われていた諸形質を細菌が操っているという新事実がもたらされた。我々が菌類として認識していた生物が、いわば菌類と細菌の共生体であるという、今までの菌学の概念を覆し、新たな生物共生系の存在を示す強いインパクトをもたらすと考えられる。さらに、腐生菌を農業上有用な共生菌に変えることが出来る可能性も示唆した。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (7 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Mortierella oedorhiza, a new species forming a dichotomously branched rhizoid at the sporangiophore base2019

    • Author(s)
      Takashima Yusuke、Degawa Yousuke、Narisawa Kazuhiko
    • Journal Title

      Mycoscience

      Volume: 60 Issue: 6 Pages: 361-365

    • DOI

      10.1016/j.myc.2019.06.007

    • NAID

      40022074032

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence and Intra-Family Phylogenetic Divergence of <i>Burkholderiaceae</i>-Related Endobacteria Associated with Species of <i>Mortierella</i>2018

    • Author(s)
      Takashima Yusuke、Seto Kensuke、Degawa Yousuke、Guo Yong、Nishizawa Tomoyasu、Ohta Hiroyuki、Narisawa Kazuhiko
    • Journal Title

      Microbes and Environments

      Volume: 33 Issue: 4 Pages: 417-427

    • DOI

      10.1264/jsme2.ME18081

    • NAID

      130007553560

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Aposymbiosis of an endofungal bacterium impacts on sexual reproduction of its fungal host.2019

    • Author(s)
      Takashima Y, Guo Y, Degawa Y, Nishizawa T, Ohta H, Narisawa K.
    • Organizer
      Asian Mycological Congress 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Comparative genomics highlights extreme genome reduction in a newly-cultured Mycoavidus-related endofungal bacterium dwelling in Mortierella parvispora.2019

    • Author(s)
      Guo Y, Takashima Y, Narisawa K, Ohta H, Nishizawa T.
    • Organizer
      ASM Microbe 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mortierella 属糸状菌に内生するMycoavidus属細菌のゲノム縮小化2019

    • Author(s)
      郭 永、高島勇介、Dilruba Sharmin、佐藤嘉則、成澤才彦、太田寛行、西澤智康
    • Organizer
      日本土壌微生物学会2019年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高温および有機態窒素条件における Mortierella humilisとその内生細菌がトマト生育に及ぼす影響2019

    • Author(s)
      松下紗季、高島勇介、西澤智康、太田寛行、成澤才彦
    • Organizer
      日本土壌微生物学会2019年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Mortierella humilis/ verticillata complexとその内生細菌のトマト生育への影響について2019

    • Author(s)
      松下紗季、高島勇介、西澤智康、太田寛行、成澤才彦
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Prevalence and phylogeny of endofungal bacteria associated with species of Mortierella.2018

    • Author(s)
      Takashima Y.
    • Organizer
      Japan-Taiwan International Joint Symposium and Workshop [The 33rd Symposium of the Kanto Branch of the Mycological Society of Japan]
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mortierella属菌とその内生細菌がトマト生育に及ぼす影響2018

    • Author(s)
      松下紗季、高島勇介、西澤智康、太田寛行、成澤才彦
    • Organizer
      日本土壌微生物学会2018年度大会
    • Related Report
      2018 Research-status Report

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Published: 2018-07-25   Modified: 2021-02-19  

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