| Project/Area Number |
18K19205
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 39:Agricultural and environmental biology and related fields
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
suzuki sakae 東京農工大学, (連合)農学研究科(研究院), 准教授 (80397017)
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| Project Period (FY) |
2018-06-29 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | 遺伝子組み換え作物 / GM作物 / 拡散防止 / メチル化 / 脱メチル化 / RNAi / カロテノイド / エピゲノム編集 / GM作物拡散防止 / DNAメチル化 / 致死性形質 |
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| Outline of Final Research Achievements |
In the present study, we tried to develop a technology for preventing GM crops-contamination by induction of autonomous lethal using control of DNA methylation system. In tobacco plants, two type of vector, target and silencer vector were introduced by co-inoculation with Agrobacterium method. The target vector has NtPDS-RNAi (inverted repeats sequence) gene controlled by PnZIP promoter (green tissue specific expression), the silencer vector has PnZIPp -RNAi (inverted repeats sequence) gene controlled by LjUBIp (strong-systemic expression). The leaf of T0 and T1 transgenic plants showed white, green or green-white mixed color. DNA methylation analysis by bisulfite sequencing indicated that, the green leaf and green-white mixed leaf plants had the target gene with DNA methylated PnZIPp sequence, and the white leaf plants had the target gene with no-methylated PnZIPp sequence. Futhermore, DNA methylation rate of cytosine in PnZIPp target sequence was more than 80%.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は，花粉の形成阻害や飛散防止などの従来技術と異なり，栽培期間中に花粉，果実，種子形成等を阻害しない，新たなGM作物の拡散防止技術の確立につながると予想される．また，本技術は，GM作物が種子形成や野生種との雑種形成を行った場合も，その次世代や雑種に対して自発的に枯死を誘導できる．本研究をさらに進展させることにより，GM作物の拡散防止技術の開発だけでなく，品種の無断増殖の防止，外来植物や雑草の駆除，他の生物種への応用が可能であると考えられる．
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