| Project/Area Number |
18K19206
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 39:Agricultural and environmental biology and related fields
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| Research Institution | Yokohama National University |
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Principal Investigator |
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| Project Period (FY) |
2018-06-29 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 植物病原菌 / さび病菌 / エフェクター / 一過性発現 / NGS解析 / 病原糸状菌 / アグロバクテリウム法 |
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| Outline of Final Research Achievements |
Various DNA extraction methods were tried on several different dried rust specimens. It was found that existing methods did not provide sufficient crushing effect and that only an all-glass homogenizer was capable of crushing the winter spores. Therefore, if the amount of specimen was not sufficient, it would be a difficult subject for this study and would be difficult to implement. Analysis of the nucleic acid samples revealed that most of them were fragmented DNA of 20 kb or less. For the transient expression system using Agrobacterium as an effector evaluation system, we found a method that can be implemented with about three times higher efficiency than the existing system by optimizing the experimental system using luciferase reporter and adding a novel bioactive compound that improves transduction efficiency.
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| Academic Significance and Societal Importance of the Research Achievements |
植物病原菌の乾燥標本という、これまでは分子生物学的研究対象とは見なされていなかった試料を用い、それらを再発掘し利用するという点が、本研究の学術的特色である。さらに、そこから得られた遺伝子情報に基づいて、植物病原菌の新規なエフェクターを探索し、植物細胞で機能する新規なDNA結合因子あるいは転写制御因子等を探索するという試みは、新規な遺伝子工学あるいは遺伝子編集技術に繋がる可能性を有しており、応用上の発展展開の可能性も秘めている。また、古い乾燥標本の保全の面にも配慮している点は、学術資料の次世代への継承という点で社会的意義もあると思われる。
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