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Elucidation of biological impacts on host plants by gut bacteria of tephritid fruit flies

Research Project

Project/Area Number 18K19217
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 39:Agricultural and environmental biology and related fields
Research InstitutionUniversity of the Ryukyus

Principal Investigator

Matsuura Yu  琉球大学, 熱帯生物圏研究センター, 助教 (80723824)

Co-Investigator(Kenkyū-buntansha) 伊藤 英臣  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (70748425)
Project Period (FY) 2018-06-29 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsミバエ / 腸内細菌 / 寄主植物 / 内部共生 / 単離培養 / FISH / ナスミバエ / ナス科野菜 / 果実 / 共生 / 分子系統解析 / 土壌 / 環境
Outline of Final Research Achievements

In this study, we first conducted a screening survey of native gut symbiotic bacteria of 11 species tephritid fruit flies in the genus Bactrocera in Okinawan islands by 16S rDNA amplicon sequencing, cultural isolation, molecular phylogenetics , FISH microscopy and insect rearing. We have found that the fly bacteriota (both culture-dependent and -independent) are dominated by several bacterial genera in the Enterobacteriaceae such as Pantoea, Enterobacter, Citrobacter, Klebsiella, and Providencia. Each population of insects from the same host plant fruit tends to harbor similar bacterial species. We have also identified more than 1633 strains by the culture-dependent assay, and representative 13 bacterial OTUs. Also, stable insect cultures were established with the artificial diet and Solanaceae fruits such as tomatoes and peppers for investigating roles of gut bacteria on the host plant utilization and environmental impacts of the gut symbioses.

Academic Significance and Societal Importance of the Research Achievements

腸内細菌は宿主動物の生存にとって非常に重要な役割を担うことが知られており、ミバエ類などの重要な害虫種を含む昆虫の適応進化にとっても欠かせない存在である。本研究により、過去に全く注目されていなかった日本の野生のミバエ類の腸内細菌叢の基本的な情報や多くの培養株を取得し、それらの感染動態を明らかにするとともに、ミバエ類が野生の植物果実にどのような影響を与えて自然界で繁栄してきたのか解明するための解析の基盤を築くことができた。今後、腸内細菌の操作を介して各腸内細菌が果たす役割を解明することで、性フェロモン合成や寄主適応などの腸内細菌の新規生物機能を利用した画期的な侵入害虫種の防除法の開発が期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (21 results)

All 2021 2020 2019 2018 Other

All Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (15 results) (of which Invited: 3 results) Remarks (2 results)

  • [Journal Article] Ophiocordyceps salganeicola, a parasite of social cockroaches in Japan and insights into the evolution of other closely-related Blattodea-associated lineages2021

    • Author(s)
      Araujo Joao P. M.、Moriguchi Mitsuru G.、Uchiyama Shigeru、Kinjo Noriko、Matsuura Yu
    • Journal Title

      IMA Fungus

      Volume: 12 Issue: 1 Pages: 1-17

    • DOI

      10.1186/s43008-020-00053-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Recurrent symbiont recruitment from fungal parasites in cicadas.2018

    • Author(s)
      Matsuura, Y., Moriyama, M., Lukasik, P., Vanderpool, D., Tanahashi, M., Meng, X. Y., McCutcheon, J. P., Fukatsu T.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 115 Issue: 26

    • DOI

      10.1073/pnas.1803245115

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites2018

    • Author(s)
      Tokuda Gaku、Mikaelyan Aram、Fukui Chiho、Matsuura Yu、Watanabe Hirofumi、Fujishima Masahiro、Brune Andreas
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 115 Issue: 51

    • DOI

      10.1073/pnas.1810550115

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] One Hundred Mitochondrial Genomes of Cicadas2018

    • Author(s)
      Lukasik Piotr、Chong Rebecca A、Nazario Katherine、Matsuura Yu、Bublitz De Anna C、Campbell Matthew A、Meyer Mariah C、Van Leuven James T、Pessacq Pablo、Veloso Claudio、Simon Chris、McCutcheon John P
    • Journal Title

      Journal of Heredity

      Volume: 110 Issue: 2 Pages: 247-256

    • DOI

      10.1093/jhered/esy068

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] アリにおける腸内共生細菌の新規伝播様式2021

    • Author(s)
      山下倫桜,松浦優,伊藤英臣,北條賢,菊池義智,下地博之
    • Organizer
      日本生態学会第68回全国大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 冬虫夏草とセミにみられる共生関係の進化2021

    • Author(s)
      松浦 優
    • Organizer
      日本農芸化学会2021年度仙台大会、大会シンポジウム「微生物研究の新機軸としての相互作用研究」
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] アリにおける腸内共生細菌の新規伝播様式2021

    • Author(s)
      山下倫桜,松浦優,伊藤英臣,北條賢,菊池義智,下地博之
    • Organizer
      第65回日本応用動物昆虫学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] セミと冬虫夏草の切っても切れない共生関係の進化2020

    • Author(s)
      松浦 優
    • Organizer
      第75回つくば進化生態学セミナー
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 沖縄産ミバエ類の腸内細菌叢の多様性解析と研究の展望2020

    • Author(s)
      松浦 優, 伊藤 英臣, 本間 淳
    • Organizer
      第64回日本応用動物昆虫学会大会(みなし開催)
    • Related Report
      2019 Research-status Report
  • [Presentation] The Ecological Plasticity of Ophiocordyceps Fungi2020

    • Author(s)
      Araujo Joao, Matsuura Yu
    • Organizer
      第64回日本応用動物昆虫学会大会(みなし開催)
    • Related Report
      2019 Research-status Report
  • [Presentation] 冬虫夏草とセミにみられる複雑な共生関係の進化2019

    • Author(s)
      松浦優
    • Organizer
      日本菌学会第63回大会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] セミ寄生性の冬虫夏草から共生菌への進化2019

    • Author(s)
      松浦 優, Araujo Joao, Vanderpool Dan, McCutcheon John
    • Organizer
      日本進化学会第21回 北海道大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 環境微生物と動物との関係を解く -現象の理解と産業への活用を目指して-2019

    • Author(s)
      伊藤英臣
    • Organizer
      令和元年度第6回TBRCセミナー
    • Related Report
      2019 Research-status Report
  • [Presentation] メタゲノム解析によって明らかにされたアリのバクテリア叢の多様性2019

    • Author(s)
      下地博之、伊藤英臣、松浦優、菊池義智
    • Organizer
      第66回日本生態学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 腸内から細胞内へ:ナガカメムシの細胞内共生進化2018

    • Author(s)
      栗原駿太、竹下和貴、松浦 優、徳田岳、曾根輝雄、伊藤英臣、菊池義智
    • Organizer
      第32回日本微生物生態学会沖縄大会
    • Related Report
      2018 Research-status Report
  • [Presentation] メタゲノムで解き明かすトゲオオハリアリの細菌叢 -社会に潜むその多様性と分布-2018

    • Author(s)
      下地博之、伊藤英臣、松浦優、菊池義智
    • Organizer
      第32回日本微生物生態学会沖縄大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Evolution of symbiotic organs in lygaeoid stinkbugs2018

    • Author(s)
      Kuechler Stefan, 松浦優
    • Organizer
      日本進化学会第20回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Hidden diversity under ground: ant- and nest-associated bacterial communities revealed by meta-genomic analyses2018

    • Author(s)
      下地博之、伊藤英臣、松浦優、菊池義智
    • Organizer
      International Union for the Study of Social Insects 2018
    • Related Report
      2018 Research-status Report
  • [Presentation] セミの共生真菌は冬虫夏草から進化した2018

    • Author(s)
      松浦優、森山実、Lukasik Piotr、Vanderpool Dan、棚橋 薫彦、孟憲英、McCutcheon John、深津 武馬
    • Organizer
      日本動物学会第89回大会2018札幌
    • Related Report
      2018 Research-status Report
  • [Remarks] 日本初のゴキブリに寄生する冬虫夏草の新種(琉球大学公式HP)

    • URL

      https://www.u-ryukyu.ac.jp/news/20003/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 研究者個人が成果を紹介しているホームページ

    • URL

      https://sm1042.skr.u-ryukyu.ac.jp/matsuura/

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2018-07-25   Modified: 2023-12-25  

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