Construction of filamentous fungal artificial chromosome and analysis of horizontal transfer and pathogenicity evolution mechanisms in the tomato wilt fungus.
| Project/Area Number |
18K19218
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Medium-sized Section 39:Agricultural and environmental biology and related fields
|
|---|
| Research Institution | Tokyo University of Science |
|---|
Principal Investigator |
ARAZOE Takayuki 東京理科大学, 理工学部応用生物科学科, 講師 (40749975)
|
|---|
| Project Period (FY) |
2018-06-29 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2021)
|
| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | トマト萎凋病菌 / ゲノム編集 / 糸状菌人工染色体 / 小型染色体 / 病原性染色体 / アクセサリー染色体 / ゲノム進化 / 水平伝播 / 病原性変異 |
|---|
| Outline of Final Research Achievements |
We developed a genome editing technology optimized for Fusarium oxysporum to enable efficient targeted gene knock-out, knock-in, base editing, and chromosomal deletion. Using this technology, we constructed a filamentous fungal artificial chromosome by removing a large proportion of the accessory chromosome, and showed the potential of the technology to carry out intracellular cloning of chromosomal regions, chromosome reconstruction, and edited chromosome transfer. Our findings and developed technologies would enable comprehensive analysis of hyphal fusion, horizontal transfer, pathogenicity evolution mechanisms, and the function of the accessory chromosomal region in F. oxysporum
|
| Academic Significance and Societal Importance of the Research Achievements |
酵母，植物，マウスおよびヒトにおける人工染色体は確立されており，これまで導入が困難であった広域な染色体領域の導入やモデルマウスの作成等，従来の研究領域を大幅に拡大させた。本研究では，糸状菌では初となる人工染色体の作出に成功し，新たなゲノム編集手法を含む遺伝子工学・染色体工学的な利用可能性を示した。また人工染色体作製に用いたゲノム領域の広域欠失誘導は，染色体領域を標的とした新たなゲノム機能解析手法として有用である。以上より，応用微生物学や植物保護科学，遺伝子工学，染色体工学等，多様な学術的・社会的分野において重要な知見や技術を得ることができた。
|
Report
(2 results)
Research Products
(17 results)
-
[Journal Article] Single crossover-mediated targeted nucleotide substitution and knock-in strategies with CRISPR/Cas9 system in the rice blast fungus2019
Author(s)
Yamato, T., Handa, A., Arazoe, T., Kuroki, M., Nozaka, A., Kamakura, T., Ohsato, S., Arie, T., Kuwata, S.
-
Journal Title
Scientific Reports
Volume: 印刷中
Issue: 1
Pages: 7427-7427
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Chloramphenicol inhibits eukaryotic Ser/Thr phosphatase and infection-specific cell differentiation in the rice blast fungus2019
Author(s)
Nozaka, A., Nishiwaki, A., Nagashima Y., Endo, S., Kuroki, M., Nakajima, M., Narukawa, M., Kamisuki, S., Arazoe, T., Taguchi, H., Sugawara, F., Kamakura T.
-
Journal Title
Scientific Reports
Volume: 印刷中
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
[Presentation] DNA double-strand breaks-mediated ectopic recombination between solo-long terminal repeats triggered pathogenic changes and genome rearrangement in the rice blast fungus2019
Author(s)
Arazoe, T., Hiraoka, D., Murochi, S., Kiguchi, K., Tanaka, T., Sakuma, T., Yamamoto, T., Arie, T., Kuwata, S., Chuma, I., Ohsato, S., Tosa, Y., Kamakura T.
Organizer
MPMI XVIII Congress
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
