• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Exploration of factors inducing heartwood formation expressed by external environmental factors

Research Project

Project/Area Number 18K19231
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
Research InstitutionKyoto University

Principal Investigator

Sakamoto Masahiro  京都大学, 農学研究科, 准教授 (40303870)

Co-Investigator(Kenkyū-buntansha) 高部 圭司  京都大学, 農学研究科, 教授 (70183449)
粟野 達也  京都大学, 農学研究科, 助教 (40324660)
中沢 威人  京都大学, 農学研究科, 助教 (80608141)
Project Period (FY) 2018-06-29 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords心材形成 / 師管転流 / ペルオキシダーゼ / プロテインキナーゼ / 情報伝達 / 師管液 / 仮道管 / シグナル伝達 / NACドメイン転写因子 / スギ / 仮道管液 / RNA-seq / 移行材
Outline of Final Research Achievements

In the previous year, sap from cedar phloem and parenchyma were concentrated and separated, and the resulting proteins were N-terminally sequenced and identified as peroxidase (POX) and protein kinase (PK). In the current year, a search of the cedar database was conducted based on the previously reported genes that have been detected and found to be present in the phloem or in the phloem sap. A database search was conducted based on the sequences of these genes. The cedar database contained 1590 PKs and 116 POXs. The search revealed two PK genes, CJt095135 and CJt115203, and two POX genes, CJt022249 and CJt027124, in POX.

Academic Significance and Societal Importance of the Research Achievements

樹木の心材形成において葉で感知された日長情報や温度センサーなどの情報が樹幹にもたらされ、心材形成の開始シグナルとなっている可能性が高いと考えた。本研究はその心材形成の開始シグナルとなる情報伝達に関与するであろう因子を検索することに意義がある。スギの師管液および仮道管液から分離したタンパクを同定した結果、ペルオキシダーゼとプロテインキナーゼが検出され、スギのデータベースを検索した結果、師管内で発現する可能性の高い遺伝子を見出すことができた。このことはスギの師管を経由した情報伝達の仕組みが心材形成に関与する可能性を示し、その改名に向けて大きな一歩となったと考える。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (26 results)

All 2023 2022 2021 2020 2019 2018

All Journal Article (13 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 13 results,  Open Access: 7 results) Presentation (13 results)

  • [Journal Article] Double-gene targeting with preassembled Cas9 ribonucleoprotein for safe genome editing in the edible mushroom<i>Pleurotus ostreatus</i>2023

    • Author(s)
      Boontawon Tatpong、Nakazawa Takehito、Choi Yeon-Jae、Ro Hyeon-Su、Oh Minji、Kawauchi Moriyuki、Sakamoto Masahiro、Honda Yoichi
    • Journal Title

      FEMS Microbiology Letters

      Volume: 370 Pages: 1-5

    • DOI

      10.1093/femsle/fnad015

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The lignin‐degrading abilities of Gelatoporia subvermispora gat1 and pex1 mutants generated via CRISPR /Cas92023

    • Author(s)
      Nakazawa Takehito、Inoue Chikako、Morimoto Ryota、Nguyen Dong Xuan、Bao Dapeng、Kawauchi Moriyuki、Sakamoto Masahiro、Honda Yoichi
    • Journal Title

      Environmental Microbiology

      Volume: - Issue: 8 Pages: 1-16

    • DOI

      10.1111/1462-2920.16372

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Introducing multiple-gene mutations in Pleurotus ostreatus using a polycistronic tRNA and CRISPR guide RNA strategy2022

    • Author(s)
      Xu Haibo、Nakazawa Takehito、Zhang Yufan、Oh Minji、Bao Dapeng、Kawauchi Moriyuki、Sakamoto Masahiro、Honda Yoichi
    • Journal Title

      FEMS Microbiology Letters

      Volume: 369 Issue: 1 Pages: 1-7

    • DOI

      10.1093/femsle/fnac102

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Targeted disruption of hir1 alters the transcriptional expression pattern of putative lignocellulolytic genes in the white-rot fungus Pleurotus ostreatus2021

    • Author(s)
      Wu H., Nakazawa T., Morimoto R., Shivani, Sakamoto M., Honda Y.
    • Journal Title

      Fungal Genet. Biol.

      Volume: 147 Pages: 103507-103507

    • DOI

      10.1016/j.fgb.2020.103507

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative transcriptional analyses of Pleurotus ostreatus mutants on beech wood and rice straw shed light on substrate-biased gene regulation2021

    • Author(s)
      Wu H., Nakazawa T., Xu H, Yang R., Bao D., Kawauchi M., Sakamoto M., Honda Y.
    • Journal Title

      Appl. Microbio. Biotechnol.

      Volume: 105 Issue: 3 Pages: 1175-1190

    • DOI

      10.1007/s00253-020-11087-9

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Functional analyses of Pleurotus ostreatus pcc1 and clp1 using CRISPR/Cas92021

    • Author(s)
      Boontawon Tatpong、Nakazawa Takehito、Horii Masato、Tsuzuki Masami、Kawauchi Moriyuki、Sakamoto Masahiro、Honda Yoichi
    • Journal Title

      Fungal Genetics and Biology

      Volume: 154 Pages: 103599-103599

    • DOI

      10.1016/j.fgb.2021.103599

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Overexpressing <i>Pleurotus ostreatus rho1b</i> results in transcriptional upregulation of the putative cellulolytic enzyme‐encoding genes observed in <i>ccl1</i> disruptants2021

    • Author(s)
      Okuda Nozomi、Nakazawa Takehito、Horii Masato、Wu Hongli、Kawauchi Moriyuki、Sakamoto Masahiro、Honda Yoichi
    • Journal Title

      Environmental Microbiology

      Volume: 23 Issue: 11 Pages: 7009-7027

    • DOI

      10.1111/1462-2920.15786

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transcriptional shifts in delignification‐defective mutants of the white‐rot fungus Pleurotus?ostreatus2020

    • Author(s)
      Wu Hongli、Nakazawa Takehito、Takenaka Atsuki、Kodera Rina、Morimoto Ryota、Sakamoto Masahiro、Honda Yoichi
    • Journal Title

      FEBS Letters

      Volume: 594 Issue: 19 Pages: 3182-3199

    • DOI

      10.1002/1873-3468.13890

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lignin Metabolic Engineering in Grasses for Primary Lignin Valorizarion2020

    • Author(s)
      Tishiaki Umezawa, Yuki Tobimatsu, Masaomi Yamamura, Takuji Miyamoto, Taichi Koshiba, Rie Takada, Pui Ying Lam, Shiro Suzuki, Masahiro Sakamoto
    • Journal Title

      Lignin

      Volume: 1 Pages: 30-41

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] OsMYB108 loss-of-function enriches p-coumaroylated and tricin lignin units in rice cell walls2019

    • Author(s)
      Takuji Miyamoto, Rie Takada, Yuki Tobimatsu, Yuli Takeda, Shiro Suzuki, Masaomi Yamamura, Keishi Osakabe, Yuriko Osakabe, Masahiro Sakamoto, Toshiaki Umezawa
    • Journal Title

      Plant Journal

      Volume: 印刷中 Issue: 6 Pages: 975-987

    • DOI

      10.1111/tpj.14290

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lignin characterization of rice CONIFERALDEHYDE 5‐HYDROXYLASE loss‐of‐function mutants generated with the CRISPR/Cas9 system.2019

    • Author(s)
      Yuri Takeda, Shiro Suzuki, Yuki Tobimatsu, Keishi Osakabe, Yuriko Osakabe, Safendrri Komara Ragamustari, Masahiro Sakamoto, Toshiaki Umezawa
    • Journal Title

      Plant Journal

      Volume: 97 Issue: 3 Pages: 543-554

    • DOI

      10.1111/tpj.14141

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Altered lignocellulose chemical structure and molecular assembly in CINNAMYL ALCOHOL DEHYDROGENASE-deficient rice2019

    • Author(s)
      Martin, A.F., Tobimatsu, Y., Kusumi, R., Matsumoto, N., Miyamoto, T., Lam, P.Y., Yamamura, M., Koshiba, T., Sakamoto, M., Umezawa, T.
    • Journal Title

      Science Reports

      Volume: 9 Issue: 1 Pages: 17153-17153

    • DOI

      10.1038/s41598-019-53156-8

    • NAID

      120006893975

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Downregulation of p‐COUMAROYL ESTER 3‐HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification2018

    • Author(s)
      Yuri Takeda, Yuki Tobimatsu,Steven D. Karlen, Taichi Koshiba, Shiro Suzuki,Masaomi Yamamura, Shinya Murakami, Mai Mukai, Takefumi Hattori, Keishi Osakabe, John Ralph, Masahiro Sakamoto, Toshiaki Umezawa
    • Journal Title

      Plant Journal

      Volume: 95 Issue: 5 Pages: 796-811

    • DOI

      10.1111/tpj.13988

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] イネ科植物におけるキシラン合成酵素遺伝子の解析2023

    • Author(s)
      坂本正弘、長谷川智彦、河内護之、中沢威人、本田与一
    • Organizer
      日本木材学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] トランスクリプトーム解析とCRISPR/Cas9を用いたヒラタケの担子奉仕形成に必須な新規遺伝子の同定2023

    • Author(s)
      小深田剛士、山崎風雅、中沢威人、菅野純子、Minji Oh、河内護之、坂本正弘、本田与一
    • Organizer
      日本木材学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 花成誘導因子Ehd1ホモログのトウチクにおける研究2021

    • Author(s)
      荒玉菜摘、吉澤敦、中沢威人、本田与一、坂本正弘
    • Organizer
      第38回日本植物バイオテクノロジー学会(つくば)大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヒラタケにおけるオートファジー関連遺伝子atg9の破壊がリグニン分解系に及ぼす影響の 解析2021

    • Author(s)
      榑林 一皓、中沢 威人、山本太一、河内護之、坂本正弘、本田与一
    • Organizer
      第65回リグニン討論会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヒラタケにおける木質成分培地上でのHap2依存的なmnp, vpの転写活性化2021

    • Author(s)
      香山 渓太、 中沢 威人、河内 護之、坂本 正弘、本田 与一
    • Organizer
      第65回リグニン討論会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヒラタケにおけるヒストン H3K36 メチル化レベルの変化が セルロース分解酵素遺伝子群の転写に及ぼす影響2021

    • Author(s)
      奥田 希実、中沢 威人、香山 渓太、河内 護之、坂本 正弘、本田 与一
    • Organizer
      第72回日本木材学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヒラタケにおける一過性遺伝子発現を利用した マーカーフリーなゲノム編集法の確立2021

    • Author(s)
      越 大志朗、河内 護之、入江 俊一、中沢 威人、坂本 正弘、本田 与一
    • Organizer
      第72回日本木材学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] スギの師細胞液および仮道管液に含まれる オリゴ糖,ペプチド,タンパク質の分析2020

    • Author(s)
      木村尚敬、粟野達也、高部圭司、坂本正弘
    • Organizer
      日本木材学会
    • Related Report
      2020 Research-status Report
  • [Presentation] キシラン側鎖糖転移酵素遺伝子の発現を変化させたイネ形質転換株における 細胞壁成分の解析2020

    • Author(s)
      長谷川智彦、宮本託志、山村正臣、中沢威人、本田与一、梅澤俊明、坂本正弘
    • Organizer
      日本木材学会
    • Related Report
      2020 Research-status Report
  • [Presentation] キシラン側鎖糖転移酵素遺伝子の発現を変化させた形質転換イネにおける 細胞壁成分の変化2020

    • Author(s)
      長谷川智彦、宮本託志、山村正臣、中沢威人、本田与一、梅澤俊明、坂本正弘
    • Organizer
      リグニン学会
    • Related Report
      2020 Research-status Report
  • [Presentation] キシラン側鎖糖転移酵素遺伝子の発現を変化させたイネ形質転換株における細胞壁成分の解析2019

    • Author(s)
      長谷川智彦、宮本託志、山村正臣、中沢威人、本田与一、梅澤俊明、坂本正弘
    • Organizer
      日本木材学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Ceriporopsis subvermisporaとヒラタケの間におけるgat1およびpex1の単独遺伝子変異がリグニン分解能および木質分解系酵素遺伝子群の転写発現に与える影響の比較解析2019

    • Author(s)
      中沢威人、井上智香子、森本亮太、徐 海博、坂本正弘、本田与一
    • Organizer
      日本木材学会
    • Related Report
      2019 Research-status Report
  • [Presentation] イネのGUX,XAT-RNAi抑制株の作成と細胞壁成分の解析2019

    • Author(s)
      長谷川智彦、宮本託志、山村正臣、中沢威人、本田与一、梅澤俊明、坂本正弘
    • Organizer
      日本木材学会
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-07-25   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi