Exploration of factors inducing heartwood formation expressed by external environmental factors
| Project/Area Number |
18K19231
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高部 圭司 京都大学, 農学研究科, 教授 (70183449)
粟野 達也 京都大学, 農学研究科, 助教 (40324660)
中沢 威人 京都大学, 農学研究科, 助教 (80608141)
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| Project Period (FY) |
2018-06-29 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 心材形成 / 師管転流 / ペルオキシダーゼ / プロテインキナーゼ / 情報伝達 / 師管液 / 仮道管 / シグナル伝達 / NACドメイン転写因子 / スギ / 仮道管液 / RNA-seq / 移行材 |
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| Outline of Final Research Achievements |
In the previous year, sap from cedar phloem and parenchyma were concentrated and separated, and the resulting proteins were N-terminally sequenced and identified as peroxidase (POX) and protein kinase (PK). In the current year, a search of the cedar database was conducted based on the previously reported genes that have been detected and found to be present in the phloem or in the phloem sap. A database search was conducted based on the sequences of these genes. The cedar database contained 1590 PKs and 116 POXs. The search revealed two PK genes, CJt095135 and CJt115203, and two POX genes, CJt022249 and CJt027124, in POX.
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| Academic Significance and Societal Importance of the Research Achievements |
樹木の心材形成において葉で感知された日長情報や温度センサーなどの情報が樹幹にもたらされ、心材形成の開始シグナルとなっている可能性が高いと考えた。本研究はその心材形成の開始シグナルとなる情報伝達に関与するであろう因子を検索することに意義がある。スギの師管液および仮道管液から分離したタンパクを同定した結果、ペルオキシダーゼとプロテインキナーゼが検出され、スギのデータベースを検索した結果、師管内で発現する可能性の高い遺伝子を見出すことができた。このことはスギの師管を経由した情報伝達の仕組みが心材形成に関与する可能性を示し、その改名に向けて大きな一歩となったと考える。
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Report
(6 results)
Research Products
(26 results)
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[Journal Article] Altered lignocellulose chemical structure and molecular assembly in CINNAMYL ALCOHOL DEHYDROGENASE-deficient rice2019
Author(s)
Martin, A.F., Tobimatsu, Y., Kusumi, R., Matsumoto, N., Miyamoto, T., Lam, P.Y., Yamamura, M., Koshiba, T., Sakamoto, M., Umezawa, T.
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Journal Title
Science Reports
Volume: 9
Issue: 1
Pages: 17153-17153
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Downregulation of p‐COUMAROYL ESTER 3‐HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification2018
Author(s)
Yuri Takeda, Yuki Tobimatsu,Steven D. Karlen, Taichi Koshiba, Shiro Suzuki,Masaomi Yamamura, Shinya Murakami, Mai Mukai, Takefumi Hattori, Keishi Osakabe, John Ralph, Masahiro Sakamoto, Toshiaki Umezawa
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Journal Title
Plant Journal
Volume: 95
Issue: 5
Pages: 796-811
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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