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Exploratory studies on toxic flatworms in association with toxification of pufferfish

Research Project

Project/Area Number 18K19238
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
Research InstitutionNihon University

Principal Investigator

ITOI Shiro  日本大学, 生物資源科学部, 准教授 (30385992)

Co-Investigator(Kenkyū-buntansha) 高井 則之  日本大学, 生物資源科学部, 准教授 (00350033)
小島 隆人  日本大学, 生物資源科学部, 教授 (60205383)
高谷 智裕  長崎大学, 水産・環境科学総合研究科(水産), 教授 (90304972)
Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywordsフグ毒 / テトロドトキシン(TTX) / オオツノヒラムシ / ツノヒラムシ属 / 多岐腸類 / 毒化機構 / ツノヒラムシ属(Planocera) / 食物連鎖 / テトロドトキシン / TTX / Planocera / COI / ヒラムシ
Outline of Final Research Achievements

PCR specific to the toxic planocerid flatworm, Planocera multitentaculata, showed that DNA encoding the COI gene was detected in the intestinal contents of TTX-bearing fishes in the waters around the Japanese Islands, suggesting that the flatworm is the major TTX supplier for TTX-bearing fishes. TTX-bearing flatworm species were seen to be restricted to specific planocerid lineages in Acotylea, suggesting that these species have common genes for TTX-accumulating mechanisms. In addition, a highly concentrated TTX-bearing species was found in cotyleran flatworm taxa.

Academic Significance and Societal Importance of the Research Achievements

本研究では、フグ毒保有魚類の毒化に有毒ヒラムシ類の寄与が大きいこと、そのヒラムシ類が分類学的にはツノヒラムシ属の特定のグループに限定されることを明らかにしたことから、今後の取り組みにより、フグ毒の蓄積に関わる重要な遺伝子の特定、さらにはフグ毒の生合成系の解明に迫ることが期待される。フグ毒の蓄積機構および生合成系の解明は、世界的に発生しているフグ毒による食中毒の発生抑制への貢献が期待されるだけでなく、わが国における養殖トラフグの安全性確保にも寄与することが期待される。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (34 results)

All 2020 2019 2018 Other

All Journal Article (11 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 5 results) Presentation (17 results) (of which Int'l Joint Research: 4 results,  Invited: 3 results) Remarks (6 results)

  • [Journal Article] Difference in tetrodotoxin content between two sympatric planocerid flatworms, Planocera multitentaculata and Planocera reticulata2020

    • Author(s)
      Shiro Itoi, Sora Tabuchi, Misato Abe, Hiroyuki Ueda, Hikaru Oyama, Ryuya Ogata, Taiki Okabe, Ayano Kishiki, Haruo Sugita
    • Journal Title

      Toxicon

      Volume: 173 Pages: 57-61

    • DOI

      10.1016/j.toxicon.2019.11.008

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The planocerid flatworm is a main supplier of toxin to tetrodotoxin-bearing fish juveniles2020

    • Author(s)
      Shiro Itoi, Tatsunori Sato, Mitsuki Takei, Riko Yamada, Ryuya Ogata, Hikaru Oyama, Shun Teranishi, Ayano Kishiki, Takenori Wada, Kaede Noguchi, Misato Abe, Taiki Okabe, Hiroyuki Akagi, Maho Kashitani, Rei Suo, Tomoko Koito, Tomohiro Takatani, Osamu Arakawa, Haruo Sugita
    • Journal Title

      Chemosphere

      Volume: 249 Pages: 126217-126217

    • DOI

      10.1016/j.chemosphere.2020.126217

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization and phylogenetic position of two sympatric sister species of toxic flatworms Planocera multitentaculata and Planocera reticulata (Platyhelminthes: Acotylea)2020

    • Author(s)
      Ryo Yonezawa, Shiro Itoi, Yoji Igarashi, Kazutoshi Yoshitake, Hikaru Oyama, Shigeharu Kinoshita, Rei Suo, Shinichi Yokobori, Haruo Sugita, Shuichi Asakawa
    • Journal Title

      Mitochondrial DNA Part B

      Volume: 未定 Issue: 3 Pages: 2352-2354

    • DOI

      10.1080/23802359.2020.1730255

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Toxic Flatworm Egg Plates Serve as a Possible Source of Tetrodotoxin for Pufferfish2019

    • Author(s)
      Taiki Okabe, Hikaru Oyama, Maho Kashitani, Yuta Ishimaru, Rei Suo, Haruo Sugita, Shiro Itoi
    • Journal Title

      Toxins

      Volume: 11 Issue: 7 Pages: 402-402

    • DOI

      10.3390/toxins11070402

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Contrasting Toxin Selectivity between the Marine Pufferfish Takifugu pardalis and the Freshwater Pufferfish Pao suvattii2019

    • Author(s)
      Gao Wei、Kanahara Yoko、Yamada Misako、Tatsuno Ryohei、Yoshikawa Hiroyuki、Doi Hiroyuki、Takatani Tomohiro、Arakawa Osamu
    • Journal Title

      Toxins

      Volume: 11 Issue: 8 Pages: 470-470

    • DOI

      10.3390/toxins11080470

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of maternal tetrodotoxin in survival of larval pufferfish2018

    • Author(s)
      Itoi Shiro、Suzuki Miwa、Asahina Kiyoshi、Sawayama Eitaro、Nishikubo Junki、Oyama Hikaru、Takei Mitsuki、Shiibashi Nanae、Takatani Tomohiro、Arakawa Osamu、Sugita Haruo
    • Journal Title

      Toxicon

      Volume: 148 Pages: 95-100

    • DOI

      10.1016/j.toxicon.2018.04.014

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Including planocerid flatworms in the diet effectively toxifies the pufferfish, Takifugu niphobles2018

    • Author(s)
      Itoi Shiro、Ueda Hiroyuki、Yamada Riko、Takei Mitsuki、Sato Tatsunori、Oshikiri Shotaro、Wajima Yoshiki、Ogata Ryuya、Oyama Hikaru、Shitto Takahiro、Okuhara Kazuya、Tsunashima Tadasuke、Sawayama Eitaro、Sugita Haruo
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 12302-12302

    • DOI

      10.1038/s41598-018-30696-z

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Maturation-associated changes in internal distribution and intra-ovarian microdistribution of tetrodotoxin in the pufferfish Takifugu pardalis2018

    • Author(s)
      Gao Wei、Kanahara Yoko、Tatsuno Ryohei、Soyano Kiyoshi、Nishihara Gregory N.、Urata Chisato、Takatani Tomohiro、Arakawa Osamu
    • Journal Title

      Fisheries Science

      Volume: 84 Issue: 4 Pages: 723-732

    • DOI

      10.1007/s12562-018-1209-2

    • NAID

      40021587964

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Stellatolide H, a cytotoxic peptide lactone from a deep-sea sponge Discodermia sp.2018

    • Author(s)
      Nakamukai Shohei、Takada Kentaro、Furihata Kazuo、Ise Yuji、Okada Shigeru、Morii Yasuhiro、Yamawaki Nobuhiro、Takatani Tomohiro、Arakawa Osamu、Gustafson Kirk R.、Matsunaga Shigeki
    • Journal Title

      Tetrahedron Letters

      Volume: 59 Issue: 26 Pages: 2532-2536

    • DOI

      10.1016/j.tetlet.2018.05.033

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] テトロドトキシンの生物学的意義とフグ毒中毒2018

    • Author(s)
      糸井史朗
    • Journal Title

      モダンメディア

      Volume: 64 Pages: 241-249

    • Related Report
      2018 Research-status Report
    • Open Access
  • [Journal Article] フグ毒TTXの生物学的意義を通してフグにTTXおけるの獲得機構を探る2018

    • Author(s)
      糸井史朗
    • Journal Title

      アグリバイオ

      Volume: 2 Pages: 397-399

    • Related Report
      2018 Research-status Report
  • [Presentation] フグ毒テトロドトキシンを保有するヒラムシのドラフトゲノム構築2020

    • Author(s)
      米澤遼、五十嵐洋治、吉武和敏、糸井史朗、周防玲、杉田治男、木下滋晴、浅川修一
    • Organizer
      令和2年度日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] クサフグの毒化に及ぼすヒラムシの卵の影響2019

    • Author(s)
      岡部泰基、尾山輝、柏谷真保、石丸悠太、周防玲、杉田治男、糸井史朗
    • Organizer
      令和元年度日本水産学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The hunt for tetrodotoxin producers from the flatworm, <I>Planocera multitentaculata</I>2019

    • Author(s)
      Haruka Yamazaki,Maho Kashitani,Yasuhito Yokoi,Taiki Okabe,Hikaru Oyama,Shiro Itoi,Haruo Sugita,Tetsusi Mori
    • Organizer
      Marine Biotechnology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Distribution of tetrodotoxin in acotylean flatworms (Platyhelminthes)2019

    • Author(s)
      Maho Kashitani,Taiki Okabe,Hikaru Oyama,Kaede Noguchi,Haruka Yamazaki,Rei Suo,Tetsushi Mori,Haruo Sugita,Shiro Itoi
    • Organizer
      Marine Biotechnology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] フグにおけるフグ毒の役割2019

    • Author(s)
      糸井史朗
    • Organizer
      第12回北陸合同バイオシンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Pufferfish ingests tetrodotoxin by feeding on toxic egg plates of the flatworm <I>Planocera multitentaculata</I>2019

    • Author(s)
      Taiki Okabe, Hikaru Oyama, Maho Kashitani, Yuta Ishimaru, Rei Suo, Haruo Sugita, Shiro Itoi
    • Organizer
      International Symposium on Aquatic Metagenomics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Towards the elucidation of the origin of tetrodotoxin (TTX): metagenomic analysis of toxic flatworm2019

    • Author(s)
      Ryo Yonezawa, Shiro Itoi, Yoji Igarashi, Kazutoshi Yoshitake, Hikaru Oyama, Shigeharu Kinoshita, Rei Suo, Shinichi Yokobori, Haruo Sugita, Shuichi Asakawa
    • Organizer
      International Symposium on Aquatic Metagenomics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 人工種苗トラフグの孵化後初期におけるTTX量の変化2019

    • Author(s)
      五十川純太、鈴木重則、荒川修、高谷智裕
    • Organizer
      令和元年度日本水産学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 石垣島米原ウモレオウギガニの地理的分布と保有毒成分2019

    • Author(s)
      Zhang Yuchengmin、上西園透生、筒井英人、山脇信博、森井康弘、荒川修、高谷智裕
    • Organizer
      令和元年度日本水産学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] TTX保有生物におけるTTXの獲得ルートと役割2019

    • Author(s)
      糸井史朗
    • Organizer
      マリンケミカルバイオロジー研究会2019
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] フグ毒テトロドトキシンを保有するヒラムシの消化管のメタゲノム解析2019

    • Author(s)
      米澤 遼、五十嵐洋治、吉武和敏、木下滋晴、糸井史朗、杉田治男、浅川修一
    • Organizer
      平成31年度日本水産学会春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] フグ毒テトロドトキシンを保有するヒラムシのミトコンドリアゲノムの解読2019

    • Author(s)
      米澤 遼、五十嵐洋治、吉武和敏、木下滋晴、糸井史朗、杉田治男、横堀伸一、浅川修一
    • Organizer
      平成31年度日本水産学会春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] トラフグ属魚類の毒化に関わるオオツノヒラムシの影響2018

    • Author(s)
      糸井史朗、尾山 輝、杉田治男
    • Organizer
      日本水産増殖学会第17回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] オオツノヒラムシの産卵期におけるTTX保有量の変動2018

    • Author(s)
      吉敷綾乃、上田紘之、糸井史朗、杉田治男
    • Organizer
      平成30年度日本水産学会秋季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] フグはフグ毒をどこから獲得し、何に使うのか?2018

    • Author(s)
      糸井史朗
    • Organizer
      第63回日本放線菌学会学術講演会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] フグ毒検査法における抽出操作の簡素化とHPLC-FL分析の有効性2018

    • Author(s)
      谷口香織、高尾秀樹、阿部敬子、辰野竜平、Gregoly N. Nishihara、阪倉良孝、高谷智裕、荒川修
    • Organizer
      日本食品衛生学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 海産フグと淡水フグのTTX/PST蓄積能2018

    • Author(s)
      山田美紗子、金原葉子、高威、土井啓行、吉川廣幸、辰野竜平、長島裕二、高谷智裕、荒川修
    • Organizer
      日本食品衛生学会
    • Related Report
      2018 Research-status Report
  • [Remarks] クサフグは毒を獲得するためにヒラムシの有毒卵を食べる

    • URL

      http://www.msr-nihon-university.org/2019/07/16/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 岡部泰基が国際メタゲノムシンポジウム2019でベストポスター賞を受賞しました

    • URL

      http://www.msr-nihon-university.org/2019/12/15/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 有毒ヒラムシ 2 種のフグ毒保有量の違い

    • URL

      http://www.msr-nihon-university.org/2020/01/26/

    • Related Report
      2019 Annual Research Report
  • [Remarks] フグ毒保有魚は、有毒ヒラムシの幼生を摂餌して毒化する

    • URL

      http://www.msr-nihon-university.org/2020/02/17/

    • Related Report
      2019 Annual Research Report
  • [Remarks] クサフグはヒラムシを食べて効果的に毒化する

    • URL

      http://www.msr-nihon-university.org/2018/08/19/press-release-7/

    • Related Report
      2018 Research-status Report
  • [Remarks] 糸井史朗准教授のフグ毒に関する研究が読売新聞で紹介されました

    • URL

      http://www.msr-nihon-university.org/2019/04/02/

    • Related Report
      2018 Research-status Report

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Published: 2018-07-25   Modified: 2021-02-19  

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