• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Novel challenging toward the molecular mechanism of fruiting-body formation starting from isolation of the endogenous active substance

Research Project

Project/Area Number 18K19241
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
Research InstitutionForest Research and Management Organization

Principal Investigator

Nishimura Takeshi  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (10353799)

Co-Investigator(Kenkyū-buntansha) 砂川 政英  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (30370282)
Project Period (FY) 2018-06-29 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsヒラタケ / 子実体誘導物質 / トリアシルグリセロール / グルコシルセラミド / 子実体形成期特異的発現遺伝子 / ハイドロホビン / キチン分解酵素 / β-1,3グルカン分解酵素 / 子実体形成機構 / 栄養菌糸 / 子実体原基 / 子実体 / 菌体抽出物 / 複合糖質 / キノコ / ホルモン様物質
Outline of Final Research Achievements

Acetone extract from three stages of mycelium, primordium, and mature fruiting body of Pleurotus ostreatus were separated and purified into fractions using silica gel column chromatography and TLC, and structurally characterized by NMR. From the screening assay for their fruiting inducing activities using paper disk, it was suggested that an endogenous fungal type glucosylceramide and triglycerides may be substances that involve in fruit-body formation of Pleurotus ostreatus at fairly low concentration. cDNA-RAPD analyses were performed with cDNAs from three stages of mycelium, primordium, and mature fruiting body. Three cDNA clones (T5, W7, A9) specifically expressed in primordium or mature fruit body were detected. Sequence analysis and database searches revealed significant similarity with hydrophobic gene of Schizophyllum commune, gene of chitin degrading enzyme in Lentinula edodes and gene of β-1,3 glucan degrading enzyme in Aspergillus nidulans.

Academic Significance and Societal Importance of the Research Achievements

キノコの子実体形成機構は今なお未解明の課題であり,その解明は学術・実用の両面で大変意義が大きい。本研究で得られたヒラタケの子実体誘導物質やその子実体形成期における特異的発現遺伝子に関する新規知見は,「キノコがいつ・どのような物質の関与によって子実体形成を行うのか」その分子基盤の確立に向けたさらなる学術的展開に大いに資すると期待される。子実体形成機構の解明はきのこの栽培技術の高度化にも資する重要課題であり,「森林・林業基本計画」(平成28年5月24日閣議決定)の「森林及び林業に関し、政府が総合的かつ計画的に講ずべき施策」中に挙げられている「山村の振興・地方創生への寄与」に対応した取組である。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (7 results)

All 2021 2020 2019 2018

All Presentation (7 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] ヒラタケ子実体アセトン抽出物からの同菌に対する子実体誘導物質の網羅的探索2021

    • Author(s)
      西村健、砂川政英
    • Organizer
      第71回日本木材学会大会研究発表要旨集, 1P195
    • Related Report
      2020 Annual Research Report
  • [Presentation] ヒラタケ菌体からのグルコシルセラミドの単離とその子実体誘導活性2020

    • Author(s)
      西村健、砂川政英
    • Organizer
      第39回日本糖質学会研究発表要旨集, C21
    • Related Report
      2020 Annual Research Report
  • [Presentation] 培養ステージの異なるヒラタケ菌体からの脂溶性・水溶性抽出物の調製と子実体誘導活性2020

    • Author(s)
      西村健、砂川政英
    • Organizer
      日本木材学会年次大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Fruit-body induction of Pleurotus ostreatus by glucosylceramide and glyceroglycolipid analogous compounds2019

    • Author(s)
      Nishimura T、Ohara S、Magae Y、Sunagawa M
    • Organizer
      25th International symposium on glycoconjugate
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Isolation of genes differentially expressed during the fruitbody development of Pleurotus ostreatus2019

    • Author(s)
      Sunagawa M、Nishimura T
    • Organizer
      Asian Mycological Congress
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 糖部分にマンノースを有するグリセロ糖脂質アナログの合成とヒラタケに対する子実体誘導活性2019

    • Author(s)
      西村健
    • Organizer
      日本木材学会
    • Related Report
      2018 Research-status Report
  • [Presentation] きのこの変異株について2018

    • Author(s)
      砂川政英、馬替由美
    • Organizer
      日本菌学会関東支部年次大会
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-07-25   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi