Imaging analysis of mechno-sensing in mitochondria of living cells
| Project/Area Number |
18K19291
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
曽我部 正博 名古屋大学, 医学系研究科, 研究員 (10093428)
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| Project Period (FY) |
2018-06-29 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ミトコンドリア / メカノバイオロジー / カルシウム / 機械刺激 / 細胞骨格 / メカニカルストレス / 小胞体 / 感知 / メカノセンサー |
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| Outline of Final Research Achievements |
Animal cell utilizes mechanosensors that sense and convert mechanical signals into biochemical signals. However, the details of those sensors or signals remain unclear. Recently mitochondria have been demonstrated to activate several intracellular signaling pathways upon mechanical stress. Therefore, we hypothesize that mitochondria can feel and respond to mechanical stress as mechanosensors. In this project, we try to verify our hypothesis and to investigate the mitochondrial signaling pathways upon mechanical stress. We found that cultured mesenchymal stem cells elevated mitochondrial calcium concentration in response to mechanical stress, resulting in increased mitochondrial ATP levels. Pharmacological studies showed that the activity of mechanosensitive channels activities was not essential to the changes in mitochondrial calcium concentration. These results support our hypothesis that mitochondria could sense mechanical stimuli and transduce them to biochemical signals.
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| Academic Significance and Societal Importance of the Research Achievements |
ミトコンドリアのメカニカルストレス感知機構は生体の各組織においても機能していると考えられ、特に、骨格筋ではそのメカノセンシング機構の破綻に伴い筋萎縮が誘導されていることが示唆されている。従って、本研究によって、メカノストレス負荷に誘導されるシグナルの欠落や変調により筋萎縮が起きるメカニズムを他の経路と区別して解析することが可能になり、廃用性筋萎縮の予防・治療方法の開発へつながる可能性がある。
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Gravity sensing in plant and animal cells.2021
Author(s)
Takahashi K, Takahashi H, Furuichi T, Toyota M, Furutani-Seiki M, Kobayashi T, Watanabe-Takano H, Shinohara M, Numaga-Tomita T, Sakaue-Sawano A, Miyawaki A, Naruse K.
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Journal Title
NPJ Microgravity
Volume: 7
Issue: 1
Pages: 2-2
DOI
Related Report
Peer Reviewed / Open Access
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