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Identification of molecules that regulate the deformability of insect cells

Research Project

Project/Area Number 18K19296
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
Research InstitutionKyoto University

Principal Investigator

Umeda Masato  京都大学, 工学研究科, 名誉教授 (10185069)

Project Period (FY) 2018-06-29 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywordsショウジョウバエ / 細胞 / 変形能 / リン脂質 / 細胞骨格 / リン脂質スクランブラーゼ / 細胞膜
Outline of Final Research Achievements

In this study, we demonstrate that Drosophila and Aedes have highly elastic cell membranes with extremely low membrane tension and high resistance to mechanical stress. In contrast to other eukaryotic cells, phospholipids are symmetrically distributed between the bilayer leaflets of the insect plasma membrane, where phospholipid scramblase (XKR) that disrupts the lipid asymmetry is constitutively active. We also demonstrate that XKR-facilitated phospholipid scrambling promotes the deformability of cell membranes by regulating both actin cortex dynamics and mechanical properties of the phospholipid bilayer. Moreover, XKR-mediated construction of elastic cell membranes is essential for hemocyte circulation in the Drosophila cardiovascular system. Deformation of mammalian cells is also enhanced by expression of Aedes XKR, thus phospholipid scrambling may contribute to formation of highly deformable cell membranes in a variety of living eukaryotic cells.

Academic Significance and Societal Importance of the Research Achievements

昆虫は地球上で最も多様化を遂げ繁栄している動物種であり、その中には進化過程において極度な小型化を達成した昆虫種も存在する。これらの微小昆虫も、大型高等動物と同様の複雑に構築された脳・神経組織、循環・消化器系を有している。従来、昆虫の小型化については、その形態学的な特性について研究が進められているが、その微小組織を構築する分子基盤についての研究はなされていない。本研究は、小型昆虫が極めて変形能が高い特異な細胞を有することを明らかにし、そのユニークな物理化学的特性を司る分子レベルでのメカニズムを明らかにした。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (18 results)

All 2020 2019 2018

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (13 results) (of which Int'l Joint Research: 3 results,  Invited: 13 results)

  • [Journal Article] Changes in the physicochemical properties of fish cell membranes during cellular senescence2020

    • Author(s)
      Akifumi Shiomi, Kohjiro Nagao, Hisae Kasai, Yuji Hara, Masato Umeda
    • Journal Title

      BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY

      Volume: 84 Issue: 3 Pages: 583-593

    • DOI

      10.1080/09168451.2019.1695576

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional expression of Δ12 fatty acid desaturase modulates thermoregulatory behaviour in Drosophila2020

    • Author(s)
      Takuto Suito, Kohjiro Nagao, Kenichi Takeuchi, Naoto Juni, Yuji Hara, Masato Umeda
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 11798-11798

    • DOI

      10.1038/s41598-020-68601-2

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of Phospholipid Flippase-mediated Activation of PIEZO1 Ion Channel in Myotube Formation2020

    • Author(s)
      原雄二、土谷正樹、平野航太郎、梅田眞郷
    • Journal Title

      Seibutsu Butsuri

      Volume: 60 Issue: 4 Pages: 222-226

    • DOI

      10.2142/biophys.60.222

    • NAID

      130007881669

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure and Function of Δ9-Fatty Acid Desaturase2019

    • Author(s)
      Nagao, K., Murakami, A., Umeda, M.
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 67 Issue: 4 Pages: 327-332

    • DOI

      10.1248/cpb.c18-01001

    • NAID

      130007621738

    • ISSN
      0009-2363, 1347-5223
    • Year and Date
      2019-04-01
    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Different mechanisms for selective transport of fatty acids using a single class of lipoprotein in Drosophila2019

    • Author(s)
      Naoya Matsuo, Kohjiro Nagao, Takuto Suito, Naoto Juni, Utako Kato, Yuji Hara, Masato Umeda
    • Journal Title

      Journal of Lipid Research

      Volume: 60 Issue: 7 Pages: 1199-1211

    • DOI

      10.1194/jlr.m090779

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 膜脂質ダイナミクスの生物機能2020

    • Author(s)
      梅田眞郷
    • Organizer
      日本脂質生化学会 特別講演
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 脂質ダイナミズムから生命現象を理解する2020

    • Author(s)
      梅田眞郷
    • Organizer
      リポクオリティ領域会議
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 脂質改変による冬眠の誘導2019

    • Author(s)
      梅田眞郷、原雄二、長尾耕治郎
    • Organizer
      理化学研究所BDR人工冬眠に向けての勉強会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 膜脂質を介する細胞内温度制御の分子機構2019

    • Author(s)
      梅田眞郷、村上光、長尾耕治郎
    • Organizer
      第71回日本細胞生物学会シンポジウム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 脂質の分子運動から生命現象を探る2019

    • Author(s)
      梅田眞郷
    • Organizer
      生化学若い研究者の会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 膜脂質を介する細胞内温度の制御機構2019

    • Author(s)
      梅田眞郷、村上光、長尾耕治郎
    • Organizer
      第57回日本生物物理学会年会シンポジウム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 昆虫から学ぶ骨格筋細胞の膜と代謝2019

    • Author(s)
      梅田眞郷
    • Organizer
      第7回若手骨格筋研究会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 生体膜の分子機構2019

    • Author(s)
      梅田眞郷
    • Organizer
      兵庫医科大学特別講演
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 生物と温度2019

    • Author(s)
      梅田眞郷
    • Organizer
      和敬論談会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Organization and deformability of insect cell membrane.2019

    • Author(s)
      Masato Umeda
    • Organizer
      The 16th International Membrane Research Forum
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 脂質ダイナミクスの生物機能2018

    • Author(s)
      梅田眞郷
    • Organizer
      第13回スフィンゴテラピィ研究会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Phospholipid flippase acts as a molecular switch for ion channel activation.2018

    • Author(s)
      Masato Umeda
    • Organizer
      Membrane Lipid Transporter Symposium 2018-Flippases, Floppases and Scramblases
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Phospholipid flip-flop as a molecular switch for ion channel activation.2018

    • Author(s)
      Masato Umeda
    • Organizer
      2nd Japan-Korea Lipid Joint Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2018-07-25   Modified: 2022-01-27  

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