| Project/Area Number |
18K19300
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
Moriya Hisao 岡山大学, 環境生命科学研究科, 准教授 (60500808)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
紀藤 圭治 明治大学, 農学部, 専任准教授 (40345632)
|
|---|
| Project Period (FY) |
2018-06-29 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
|---|
| Keywords | 細胞 / 過剰発現 / 細胞内輸送 / 酵母 / 処理能力 / タンパク質負荷 / 増殖阻害 / 細胞の処理能力 |
|---|
| Outline of Final Research Achievements |
In the process of expressing their functions, proteins undergo various processes such as synthesis, folding, transport, and degradation. These processes are thought to have different processing capacities, depending on the amount of resources devoted to them. However, the processing capacity of intracellular processes has never been investigated before. In this study, we identified the proteins that are processed by specific processes in budding yeast with the highest critical expression levels by using the genetic tug-of-war method, which measures the critical expression level of proteins, and protein quantification. By using these proteins as indicator proteins, the ability to process intracellular processes, especially protein synthesis and transport processes, was clarified.
|
| Academic Significance and Societal Importance of the Research Achievements |
細胞がタンパク質生産工場である限り、原理的には、どんなタンパク質でも究極的に大量発現するとプロセスの負荷が生じ、増殖阻害を起こすはずである。一方、これまで過剰発現による増殖阻害のメカニズムは、細胞の処理能力をまったく加味せず議論されてきた。あるタンパク質の過剰が増殖阻害を起こすことが見つかった時、それが処理能力に過負荷をかけていない事が分かって初めて、そのタンパク質に特異的なメカニズムを議論することができる。したがって、本研究の成果は過剰発現の考え方を根底から変える可能性がある。
|
