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Cell processing capacity explored by expression limits of proteins

Research Project

Project/Area Number 18K19300
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
Research InstitutionOkayama University

Principal Investigator

Moriya Hisao  岡山大学, 環境生命科学研究科, 准教授 (60500808)

Co-Investigator(Kenkyū-buntansha) 紀藤 圭治  明治大学, 農学部, 専任准教授 (40345632)
Project Period (FY) 2018-06-29 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords細胞 / 過剰発現 / 細胞内輸送 / 酵母 / 処理能力 / タンパク質負荷 / 増殖阻害 / 細胞の処理能力
Outline of Final Research Achievements

In the process of expressing their functions, proteins undergo various processes such as synthesis, folding, transport, and degradation. These processes are thought to have different processing capacities, depending on the amount of resources devoted to them. However, the processing capacity of intracellular processes has never been investigated before. In this study, we identified the proteins that are processed by specific processes in budding yeast with the highest critical expression levels by using the genetic tug-of-war method, which measures the critical expression level of proteins, and protein quantification. By using these proteins as indicator proteins, the ability to process intracellular processes, especially protein synthesis and transport processes, was clarified.

Academic Significance and Societal Importance of the Research Achievements

細胞がタンパク質生産工場である限り、原理的には、どんなタンパク質でも究極的に大量発現するとプロセスの負荷が生じ、増殖阻害を起こすはずである。一方、これまで過剰発現による増殖阻害のメカニズムは、細胞の処理能力をまったく加味せず議論されてきた。あるタンパク質の過剰が増殖阻害を起こすことが見つかった時、それが処理能力に過負荷をかけていない事が分かって初めて、そのタンパク質に特異的なメカニズムを議論することができる。したがって、本研究の成果は過剰発現の考え方を根底から変える可能性がある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (29 results)

All 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (9 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 9 results,  Open Access: 8 results) Presentation (16 results) (of which Int'l Joint Research: 9 results,  Invited: 2 results) Remarks (2 results)

  • [Int'l Joint Research] トロント大学(カナダ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] トロント大学(カナダ)

    • Related Report
      2019 Research-status Report
  • [Journal Article] N-terminal deletion of Swi3 created by the deletion of a dubious ORF YJL175W mitigates protein burden effect in S. cerevisiae2020

    • Author(s)
      Saeki Nozomu、Eguchi Yuichi、Kintaka Reiko、Makanae Koji、Shichino Yuichi、Iwasaki Shintaro、Kanno Manabu、Kimura Nobutada、Moriya Hisao
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 9500-9500

    • DOI

      10.1038/s41598-020-66307-z

    • NAID

      120006883501

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Exploring the Complexity of Protein-Level Dosage Compensation that Fine-Tunes Stoichiometry of Multiprotein Complexes2020

    • Author(s)
      Ishikawa Koji、Ishihara Akari、Moriya Hisao
    • Journal Title

      PLOS Genetics

      Volume: 16 Issue: 10 Pages: e1009091-e1009091

    • DOI

      10.1371/journal.pgen.1009091

    • NAID

      120006937515

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genetic profiling of protein burden and nuclear export overload.2020

    • Author(s)
      Kintaka R, Makanae K, Namba S, Kato H, Kito K, Ohnuki S, Ohya Y, Andrews BJ, Boone C, Moriya H.
    • Journal Title

      Elife

      Volume: 9

    • DOI

      10.7554/elife.54080

    • NAID

      120006941058

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparative gene analysis focused on silica cell wall formation: Identification of diatom-specific SET domain protein methyltransferases2020

    • Author(s)
      Nemoto, M., Iwaki, S., Moriya, H., Monden, Y., Tamura, T., Inagaki, K., Mayama, S. and Obuse K.
    • Journal Title

      Marine Biotechnology

      Volume: - Issue: 4 Pages: 551-563

    • DOI

      10.1007/s10126-020-09976-1

    • NAID

      120007045009

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an experimental method of systematically estimating protein expression limits in HEK293 cells2020

    • Author(s)
      Mori Yoshihiro、Yoshida Yuki、Satoh Ayano、Moriya Hisao
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1

    • DOI

      10.1038/s41598-020-61646-3

    • NAID

      120006883502

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The expression level and cytotoxicity of green fluorescent protein are modulated by an additional N-terminal sequence2020

    • Author(s)
      Moriya Hisao、Graduate School of Environmental and Life Sciences, Research Core for Interdisciplinary Sciences, Okayama University
    • Journal Title

      AIMS Biophysics

      Volume: 7 Issue: 2 Pages: 121-132

    • DOI

      10.3934/biophy.2020010

    • NAID

      120006860108

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis122019

    • Author(s)
      Abe-Kanoh Naomi、Kunisue Narumi、Myojin Takumi、Chino Ayako、Munemasa Shintaro、Murata Yoshiyuki、Satoh Ayano、Moriya Hisao、Nakamura Yoshimasa
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 8866-8866

    • DOI

      10.1038/s41598-019-45248-2

    • NAID

      120006648600

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estimating the Protein Burden Limit of Yeast Cells by Measuring Expression Limits of Glycolytic Proteins.2018

    • Author(s)
      Eguchi, Y., Makanae, K., Hasunuma, T., Ishibashi, Y., Kito, K., Moriya, H.
    • Journal Title

      Elife

      Volume: 7

    • DOI

      10.7554/elife.34595

    • NAID

      120006501235

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genetic Analysis of Signal Generation by the Rgt2 Glucose Sensor of Saccharomyces cerevisiae2018

    • Author(s)
      Scharff-Poulsen Peter、Moriya Hisao、Johnston Mark
    • Journal Title

      G3

      Volume: 8 Issue: 8 Pages: 2685-2696

    • DOI

      10.1534/g3.118.200338

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 過剰発現が適応的に働く遺伝子の体系的同定2020

    • Author(s)
      守屋央朗、佐伯
    • Organizer
      日本進化学会第22回オンライン大会
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] システインを含むタンパク質の過剰発現は出芽酵母の形態異常をもたらす2020

    • Author(s)
      加藤寿明、守屋央朗
    • Organizer
      酵母遺伝学フォーラム第53回研究報告会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 多コピー化が高塩ストレス下で適応的にはたらく遺伝子の体系的探索2020

    • Author(s)
      佐伯望、守屋央朗
    • Organizer
      酵母遺伝学フォーラム第53回研究報告会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 3XGFPの過剰発現が引き起こす増殖阻害現象2020

    • Author(s)
      難波匠太郎、金高令子、守屋央朗
    • Organizer
      酵母遺伝学フォーラム第53回研究報告会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 高塩ストレス下で多コピー化が適応的に機能する出芽酵母遺伝子の体系的探索2020

    • Author(s)
      佐伯望、守屋央朗
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 出芽酵母のデノボ遺伝子の機能解析2020

    • Author(s)
      守屋央朗
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 過剰発現が適応的に働く遺伝子の体系的同定2019

    • Author(s)
      守屋央朗
    • Organizer
      日本進化学会第21回大会
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Genetic profiling of protein burden and nuclear export overload2019

    • Author(s)
      金高令子、Charlie Boone、守屋央朗
    • Organizer
      ICYGMB 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Systematic Identification of Genes whose Overexpression Positively Affect Fitness in Various Environments2019

    • Author(s)
      佐伯望、守屋央朗
    • Organizer
      ICYGMB 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 過剰発現実験により探るプロテオームの拘束条件2019

    • Author(s)
      守屋央朗
    • Organizer
      日本遺伝学会第91回大会
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Systematic Identification of Genes Whose Overexpression Works Adaptively Using the ADOPT System2019

    • Author(s)
      佐伯望、守屋央朗
    • Organizer
      The 20th International Conference on Systems Biology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Defining harmful yeast oroteins by mesuring overexpression limits2018

    • Author(s)
      江口優一、守屋央朗
    • Organizer
      2018 Yeast Genetics Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] タンパク質の限界発現量から探る細胞の処理能力2018

    • Author(s)
      守屋央朗、加藤有香
    • Organizer
      酵母遺伝学フォーラム第51回研究報告会
    • Related Report
      2018 Research-status Report
  • [Presentation] タンパク質の発現限界を測る遺伝学的手法TOW-Fu2018

    • Author(s)
      江口優一、守屋央朗
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 過剰発現の限界から探る細胞の処理能力2018

    • Author(s)
      守屋央朗、江口優一、蒔苗浩司、加藤有香
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 多コピー化が増殖に有利に働く酵母遺伝子のハイスループットスクリーニング2018

    • Author(s)
      佐伯望、江口優一、堀内智司、Herbst Konrad、Kirrmaier Daniel、Knop Michael 、守屋央朗
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report
  • [Remarks] 岡山大学異分野融合先端研究コア・システム細胞学研究室

    • URL

      https://tenure5.vbl.okayama-u.ac.jp/HMlab/

    • Related Report
      2020 Annual Research Report 2019 Research-status Report 2018 Research-status Report
  • [Remarks] 酵母とシステムバイオロジー(ブログ)

    • URL

      https://tenure5.vbl.okayama-u.ac.jp/HM_blog/

    • Related Report
      2020 Annual Research Report 2019 Research-status Report

URL: 

Published: 2018-07-25   Modified: 2022-01-27  

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