• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Platform for light-inducible, biochemical labeling of ribosomes in living single cells for the novel single-cell analytics based on seamlessly connected live cell imaging and transcriptomics

Research Project

Project/Area Number 18K19313
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Komatsu Naoki  国立研究開発法人理化学研究所, 脳神経科学研究センター, 基礎科学特別研究員 (30737440)

Project Period (FY) 2018-06-29 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywordsビオチン化 / 光操作 / 生細胞イメージング / 光遺伝学 / 蛍光タンパク質 / リボソーム / 1細胞解析 / タンパク質ビオチン化 / ライブイメージング / 遺伝子発現解析 / RNA-seq / リボソームプロファイリング
Outline of Final Research Achievements

We tried to develop a technical element that would contribute the development of new methodologies that enable gene expression analysis for any cells identified by fluorescence live cell imaging while circumventing the need for a cell detachment process. In particular, we tried to develop a light-activated biotinyltransferase that can be activated by light illumination and chemically modify intracellular protein by biotinylation only in cells illuminated. We noticed that tested candidate molecules showed high background of biotinylation before light illumination. To lower the background, we designed other candidate molecules based on different working principles and constructed expression plasmids for the designed molecules. Further characterization and optimization of the candidate molecules will be performed in future.

Academic Significance and Societal Importance of the Research Achievements

多細胞からなる組織や細胞集団の性質に関する理解を更に進めていくにあたり、細胞内のmRNAやタンパク質を1細胞レベルかつ網羅的に解析する手法の高度化・多岐化が求められている。生細胞を標識するための、光と化学修飾を組み合わせた新たな手法について、本研究の実施によりその開発基盤を構築することができた。開発を今後進めることにより、多細胞集団を1細胞解析するための新たな手法が構築されることが期待される。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (7 results)

All 2021 2020 2019

All Journal Article (1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Single-Cell Live Imaging2019

    • Author(s)
      Toru Hiratsuka and Naoki Komatsu
    • Journal Title

      Single Cell Methods

      Volume: - Pages: 409-421

    • DOI

      10.1007/978-1-4939-9240-9_24

    • ISBN
      9781493992393, 9781493992409
    • Related Report
      2018 Research-status Report
  • [Presentation] mTORC1活性動態の細胞周期進行における生物学的意義の解明2021

    • Author(s)
      小松 直貴
    • Organizer
      第73回日本細胞生物学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 細胞周期依存的なmTORC1活性波の可視化2021

    • Author(s)
      小松 直貴
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] mTORC1活性動態の細胞周期進行における生物学的意義の解明2020

    • Author(s)
      小松 直貴
    • Organizer
      多次元細胞計測ワークショップ
    • Related Report
      2019 Research-status Report
  • [Presentation] Unraveling mTORC1 activity dynamics and its biological roles in cell cycle progression2019

    • Author(s)
      Naoki Komatsu, Sakaue-Sawano Asako, Atsushi Miyawaki
    • Organizer
      ResonanceBio International Symposium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Unraveling mTORC1 activity dynamics and its biological roles in cell cycle progression2019

    • Author(s)
      Naoki Komatsu, Sakaue-Sawano Asako, Atsushi Miyawaki
    • Organizer
      ASCB|EMBO 2019 Meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 癌細胞増殖シグナルのライブイメージング2019

    • Author(s)
      小松 直貴
    • Organizer
      理研シンポジウム「細胞システムの動態と論理XI」
    • Related Report
      2018 Research-status Report
    • Invited

URL: 

Published: 2018-07-25   Modified: 2023-03-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi