| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
The purpose of this study is to identify cells that secrete exosomes by inserting timing- and site-specific surface proteins into the surface of exosomes, known as extracellular vesicles, and identifying the origin of exosomes by purifying the timing- and site-specific surface proteins. We have succeeded in generating mice lacking endogenous CD9 by genome editing, and in constructing a system that expresses CD9-EGFP, a fusion of human CD9 and EGFP, in a timing- and site-specific manner and even generated chimeric transgenic mice using ES cells transfected with this system. But, all chimeric mice were found to be infertile due to impaired spermatogenesis. It is possible that the omission of CD9-EGFP expression affected spermatogenesis.
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