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Mechanism involved in the biosynthesis of a sex-inducing substance in the planarian Dugesia ryukyuensis

Research Project

Project/Area Number 18K19318
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
Research InstitutionHirosaki University

Principal Investigator

Kobayashi Kazuya  弘前大学, 農学生命科学部, 教授 (50360110)

Project Period (FY) 2018-06-29 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsプラナリア / 有性生殖 / 無性生殖 / 生殖様式転換 / 共生細菌
Outline of Final Research Achievements

Metazoans occasionally switch their mode of reproduction on the basis of environmental changes, life cycle phase, or both. However, the mechanisms underlying the switch from an asexual to a sexual mode of reproduction and vice versa are poorly understood. We established an assay system for sexualization in the planarian Dugesia ryukyuensis. In this assay system, asexual worms were stimulated to develop hermaphroditic reproductive organs by being fed sexually mature planarians. This means that sexually mature planarians contain sex-inducing substances. In the current study, using this assay system, we suggested a mechanism involved in the biosynthesis of a sex-inducing substance.

Academic Significance and Societal Importance of the Research Achievements

本研究では扁形動物プラナリアに、宿主の生殖細胞の分化を抑制して「無性化」を促進する共生細菌が存在し有性化を阻害する、いわゆる「性殺し(有性排除)」という仕組みを新たな概念として提案することができた。将来的にハエやマウスなどの生殖細胞分化でも検証することで、生殖細胞を誘導する仕組みに共生細菌の存在が関与しているという、生物学全体にインパクトを与える新しい共通原理を提案できるかもしれない。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2021 2020 2019 2018

All Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] D-Tryptophan enhances the reproductive organ-specific expression of the amino acid transporter homolog Dr-SLC38A9 involved in the sexual induction of planarian Dugesia ryukyuensis2021

    • Author(s)
      Takanobu Maezawa, Masaki Ishikawa, Kiyono Sekii, Go Nagamatsu, Ryohei Furukawa, Kazuya Kobayashi
    • Journal Title

      Zoological Letters

      Volume: 7 Issue: 1 Pages: 4-4

    • DOI

      10.1186/s40851-021-00173-z

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sex-inducing substances terminate dormancy in planarian postembryonic reproductive development2020

    • Author(s)
      Kiyono Sekii, Kazuya Kobayashi
    • Journal Title

      Advances in Invertebrate (Neuro) Endocrinology, Volume 1: Phyla Other Than Arthropoda

      Volume: 1 Pages: 25-61

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptomic analysis reveals differences in the regulation of amino acid metabolism in asexual and sexual planarians2019

    • Author(s)
      Sekii Kiyono, Yorimoto Shunta, Okamoto Hikaru, Nagao Nanna, Maezawa Takanobu, Matsui Yasuhisa, Yamaguchi Katsushi, Furukawa Ryohei, Shigenobu Shuji, Kobayashi Kazuya
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 6132-6132

    • DOI

      10.1038/s41598-019-42025-z

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A comprehensive comparison of sex-inducing activity in asexual worms of the planarian Dugesia ryukyuensis: the crucial sex-inducing substance appears to be present in yolk glands in Tricladida2018

    • Author(s)
      Nakagawa Haruka、Sekii Kiyono、Maezawa Takanobu、Kitamura Makoto、Miyashita Soichiro、Abukawa Marina、Matsumoto Midori、Kobayashi Kazuya
    • Journal Title

      Zoological Letters

      Volume: 4 Issue: 1 Pages: 14-14

    • DOI

      10.1186/s40851-018-0096-9

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] プラナリア有性化因子合成酵素の単離を目指して2019

    • Author(s)
      齋藤伊玖真・齋藤由梨亜・関井清乃・古川亮平・小柳亮・佐藤矩行・小林一也
    • Organizer
      日本動物学会 令和元年度東北支部大会
    • Related Report
      2019 Research-status Report
  • [Presentation] プラナリア有性化因子の給餌刺激で発現が誘導される遺伝子について2019

    • Author(s)
      黒田道生・成田美優・萬田冴佳・頼本隼汰・山口勝司・重信秀治・古川亮平・関井清乃・小林一也
    • Organizer
      日本動物学会第90回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] プラナリア有性化におけるアミノ酸の役割2019

    • Author(s)
      清水辰海・江口碧唯・石川正樹・関井清乃・古川亮平・小林一也・石田哲夫・前澤孝信
    • Organizer
      日本動物学会第90回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Mechanisms in ovarian development during sexual induction of the planarian Dugesia ryukyuensis2018

    • Author(s)
      Maezawa Takanobu, Shimizu Tatsumi, Egawa Aoi, Ishikawa Masaki, Sekii Kiyono, Kobayashi Kazuya
    • Organizer
      2018 International Planarian Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] プラナリア生殖腺発達におけるFGFシグナルの機能解析2018

    • Author(s)
      加納沙也佳・関井清乃・小林一也・梅園良彦・織井秀文
    • Organizer
      日本動物学会第89回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] プラナリア有性化過程における卵巣形成機構2018

    • Author(s)
      前澤孝信・清水辰海・江川碧唯・石川正樹・関井清乃・小林一也
    • Organizer
      日本動物学会第89回大会
    • Related Report
      2018 Research-status Report
  • [Book] Reproductive and Developmental Strategies The Continuity of Life2018

    • Author(s)
      Kazuya Kobayashi, Takeshi Kitano, Yasuhiro Iwao, Mariko Kondo
    • Total Pages
      789
    • Publisher
      Spriger
    • ISBN
      9784431566076
    • Related Report
      2018 Research-status Report

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Published: 2018-07-25   Modified: 2022-01-27  

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