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Investigation of the relationship between positional information and cell identity by controlling the nuclear dynamics using optical manipulation

Research Project

Project/Area Number 18K19331
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
Research InstitutionNagoya University

Principal Investigator

Kurihara Daisuke  名古屋大学, トランスフォーマティブ生命分子研究所, 特任講師 (90609439)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords植物 / 雌性配偶体 / 細胞個性 / 位置情報 / 顕微操作 / イメージング / 光顕微操作 / シロイヌナズナ / 核動態
Outline of Final Research Achievements

In this study, we aimed to clarify the relationship between nuclear positional information and cell fate specification to elucidate the molecular mechanisms of cell fate specification in plants using a simple female gametophyte formation process with a small number of cells. We established the in vitro female gametophyte development system of Arabidopsis and monitored the dynamics of female gametophyte development in real-time. Analysis of the spatiotemporal information of nuclear dynamics and the expression of cell fate marker during the female gametophyte development suggested that the cell fate specification initiated earlier than previously thought. We also clarified the problem to be overcome in the intracellular organelle manipulation technology in the plant cells.

Academic Significance and Societal Importance of the Research Achievements

被子植物の雌性配偶体形成過程における細胞個性決定機構は、細胞間コミュニケーションを介した機構と予想され、非常に魅力的なモデル現象としても注目され、以前より世界中で解析が行われているが、これまで花の奥深くで起こる現象であるため、突然変異体を用いた解析しか行うことができなかった。本研究で確立したin vitro胚珠培養系を用いたライブセル解析により、リアルタイムに雌性配偶体形成への影響を解析することがはじめて可能となったことで、細胞個性決定機構の解明に繋がることが期待される。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (7 results)

All 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Int'l Joint Research] デュッセルドルフ大学(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ミラノ大学(イタリア)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Live-Cell Imaging of Zygotic Intracellular Structures and Early Embryo Pattern Formation in Arabidopsis thaliana2020

    • Author(s)
      Ueda Minako、Kimata Yusuke、Kurihara Daisuke
    • Journal Title

      Methods in Molecular Biology

      Volume: 2122 Pages: 37-47

    • DOI

      10.1007/978-1-0716-0342-0_4

    • ISBN
      9781071603413, 9781071603420
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamics of the cell fate specifications during female gametophyte development in Arabidopsis2020

    • Author(s)
      Susaki Daichi、Suzuki Takamasa、Maruyama Daisuke、Ueda Minako、Higashiyama Tetsuya、Kurihara Daisuke
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2020.04.07.023028

    • Related Report
      2019 Annual Research Report
    • Open Access
  • [Presentation] Live cell analysis and deep imaging for plant development2019

    • Author(s)
      Daisuke Kurihara
    • Organizer
      JSOL2019 (第16回日本ナス科コンソーシアム年会)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Live-cell analysis of female gametophyte development in Arabidopsis2019

    • Author(s)
      Daisuke Kurihara, Daichi Susaki, Tetsuya Higashiyama
    • Organizer
      Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Live-cell analysis of female gametophyte development in Arabidopsis2018

    • Author(s)
      Daisuke Kurihara, Daichi Susaki, Tetsuya Higashiyama
    • Organizer
      the 25th International Congress on Sexual Plant Reproduction
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-07-25   Modified: 2021-02-19  

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