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Identification of genes involved in germline sex determination by global screening

Research Project

Project/Area Number 18K19332
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
Research InstitutionNagoya University

Principal Investigator

Nishimura Toshiya  名古屋大学, 理学研究科, 助教 (10758056)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords生殖細胞 / ゲノム編集 / メダカ / CRISPR/Cas9
Outline of Final Research Achievements

Germ cells are the only cells that can generate the next generation and produce either sperm and eggs. In this study, a genetic screening system was constructed to elucidate the mechanism of sperm-egg fate decision in germ cells using medaka. First, comparative gene expression analyses were performed between male and female germ cells to narrow down the number of candidate genes. Second, an efficient generation of DNA templates for gRNAs in CRISPR/Cas9 system was established. Third, transgenic lines in which spermatogenesis and oogenesis could be visualized by fluorescence were established. Upon gene knock-out, the phenotype could be easily distinguished by observing alive medaka under a fluorescent microscopy.

Academic Significance and Societal Importance of the Research Achievements

生殖細胞がどのような仕組みで精子あるいは卵になるか(生殖細胞の性決定)は、医学や水畜産学分野において基本的な問題であるにも関わらずほとんど分かっていないのが現状である。メダカを用いた研究で脊椎動物で初めてfoxl3という生殖細胞の性決定遺伝子が同定された。本研究では、foxl3を中心とした生殖細胞の性の仕組みを具体的に明らかにするために、効率的に生殖細胞の性に関わる遺伝子群のスクリーニング可能な実験系を構築した。本手法は生きた個体を用いて簡便に遺伝子の働きを探ることが出来るため、生殖生物学分野の研究だけでなく、生物学分野全般の研究において役立つツールだと考えられる。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2020 2019 2018

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Seasonal changes in NRF2 antioxidant pathway regulates winter depression-like behavior2020

    • Author(s)
      Nakayama T, Okimura K, Shen J, Guh Y-J, Tamai K, Shimada A, Minou S, Okushi Y, Shimmura T, Furukawa Y, Kadofusa N, Sato A, Nishimura T, Tanaka M, Nakayama K, Shiina N, Yamamoto N, Loudon A.S, Nishiwaki-Ohkawa T, Shinomiya A, Nabeshima T, Nakane Y, and Yoshimura T
    • Journal Title

      PNAS

      Volume: - Issue: 17 Pages: 1-10

    • DOI

      10.1073/pnas.2000278117

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Starvation causes female-to-male sex reversal through lipid metabolism in the teleost fish, medaka (Olyzias latipes)2020

    • Author(s)
      Sakae Yuta、Oikawa Akira、Sugiura Yuki、Mita Masatoshi、Nakamura Shuhei、Nishimura Toshiya、Suematsu Makoto、Tanaka Minoru
    • Journal Title

      Biology Open

      Volume: 9 Issue: 4 Pages: 1-12

    • DOI

      10.1242/bio.050054

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel components of germline sex determination acting downstream of foxl3 in medaka2019

    • Author(s)
      Kikuchi Mariko、Nishimura Toshiya、Saito Daisuke、Shigenobu Shuji、Takada Ritsuko、Gutierrez-Triana Jos? Arturo、Cerd?n Juan Luis Mateo、Takada Shinji、Wittbrodt Joachim、Suyama Mikita、Tanaka Minoru
    • Journal Title

      Developmental Biology

      Volume: 445 Issue: 1 Pages: 80-89

    • DOI

      10.1016/j.ydbio.2018.10.019

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Seasonal regulation of the lncRNA LDAIR modulates self-protective behaviours during the breeding season2019

    • Author(s)
      Nakayama T, Shimmura T, Shinomiya A, Okimura K, Takehana Y, Furukawa Y, Shimo T, Senga T, Nakatsukasa M, Nishimura T, Tanaka M, Okubo K, Kamei Y, Naruse K, Yoshimura T
    • Journal Title

      Nature Ecology & Evolution

      Volume: 3 Pages: 845-852

    • NAID

      120006718405

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Germline sex determination by nanos3, a component of germ plasm, in medaka2018

    • Author(s)
      Nishimura T and Tanaka M
    • Organizer
      4th Strategic Meeting for Medaka Research 3rd Regional Fish Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-07-25   Modified: 2021-02-19  

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