Cell lineage analysis by using post-zygotic spontaneous mutations
| Project/Area Number |
18K19340
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2018-06-29 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 突然変異 / 次世代シーケンサー / 細胞系譜 / モザイク変異 / 胚発生 / マウス |
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| Outline of Final Research Achievements |
For understanding the developmental system of multicellular organisms, it is important to understand the cell lineages during the developmental stage. In this study, whole-genome sequencing was performed on adult mouse tissue samples to accurately identify mutations naturally occurring during early development (observed as mosaic-state mutations in tissues). By measuring their variant allelle frequencies and analyzing them with methmatical model, we succeeded in developing a new methodology that enables to reconstruct early embryonic cell lineages.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で開発に成功した「細胞系譜の解析技術」は、既存の方法に比べて、大きな優位性をもつが、シーケンシング時のカバレッジを増加させれば、さらに詳細な細胞系譜図の構築が可能になる。また、遺伝子組み換え技術を必要としないため、ヒト検体でも、すぐに応用可能な方法論である。医学研究も含めて、今後の生命科学の様々な領域で活用されていくと考えられる技術であり、学術的にも社会的にも意義の大きい研究だと考えられる。
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Characteristics of induced mutations in offspring derived from irradiated mouse spermatogonia and mature oocytes2020
Author(s)
Yasunari Satoh, Jun-ichi Asakawa, Mayumi Nishimura, Tony Kuo, Norio Shinkai, Harry M. Cullings, Yohei Minakuchi, Jun Sese, Atsushi Toyoda, Yoshiya Shimada, Nori Nakamura, Arikuni Uchimura
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Journal Title
Scientific Reports
Volume: 10
Issue: 1
Pages: 37-37
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Mutation spectrum in the genome of offspring derived from irradiated mouse spermatogonia and mature oocytes2019
Author(s)
Yasunari Satoh, Mayumi Nishimura, Tony Kuo, Norio Shinkai, Harry M. Cullings, Yohei Minakuchi, Jun Sese, Atsushi Toyoda, Yoshiya Shimada, Nori Nakamura, Arikuni Uchimura
Organizer
日本放射線影響学会第62回大会
Related Report
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