Longevity control associated with plant reproduction

• Project/Area Number: 18K19342
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Ito Toshiro 奈良先端科学技術大学院大学, 先端科学技術研究科, 教授 (90517096)
• Co-Investigator(Kenkyū-buntansha): 北口 哲也 東京工業大学, 科学技術創成研究院, 准教授 (60432374)
• Project Period (FY): 2018-06-29 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: プログラムセルデス / 発現制御 / 幹細胞 / 増殖抑制 / ヒストン修飾 / イメージング

Outline of Final Research Achievements

In Arabidopsis flower development, we show that stem cell activities are actively terminated by multiple genetic pathways (Nature Comm 2018; EMBO J. 2018; Plant Cell 2019). To understand how program cell death (PCD) is involved in reproductive development, we established PCD reporter lines and examined their expression patterns. PCD reporters are induced in mature shoot apical meristems (SAMs) and fully grown mature petals. By performing RNA-seq in immature and mature SAMs and petals, we identified signaling pathways genes in reactive oxygen species (ROS) and jasmonic acid (JA), respectively. These results show that PCD happens in plant reproduction through multiple signaling cascades.

Academic Significance and Societal Importance of the Research Achievements

花がおしべやめしべをつくり、次世代の種を残すためには、花の元となる幹細胞が十分に増殖した後に、増殖を止める必要がある。私達は生殖にともなっておきる植物のプログラム細胞死について解析した結果、新たに、茎頂や花弁においてプログラム細胞死が起きていることがわかった。茎頂においてはROSシグナル系路が、花弁においてはジャスモン酸シグナル系路が特異的に誘導されていた。プログラム細胞死が調節できるようになれば、園芸植物の花の改良や穀物類の増産などが期待できる。

Research Products

• [Journal Article] Effects of cold atmospheric plasma irradiation on Arabidopsis seedlings (2019)
  • Author(s): Kobayashi Mime、Wang Yukun、Kumagai Shinya、Uraoka Yukiharu、Ito Toshiro
  • Journal Title: Japanese Journal of Applied Physics Volume: 59 Issue: SA Pages: SAAB09-SAAB09
  • DOI: 10.7567/1347-4065/ab4e7b
  • NAID: 210000157378
  • Peer Reviewed
• [Journal Article] CRABS CLAW and SUPERMAN coordinate hormone-, stress- and metabolic-related gene expression during Arabidopsis stamen development (2019)
  • Author(s): Lee, H. Z., Tatsumi, Y. Ichihashi, Y., Suzuki, T., Shibata, A., Shirasu, K., Yamaguchi, N., Ito, T.
  • Journal Title: Front Plant Sci. Volume: 7 Pages: 437-437
  • DOI: 10.3389/fevo.2019.00437
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional Dissection of Class C Genes in Rice (2019)
  • Author(s): Ito Toshiro
  • Journal Title: Plant and Cell Physiology Volume: 60 Issue: 6 Pages: 1164-1165
  • DOI: 10.1093/pcp/pcz093
  • NAID: 120007123945
  • Peer Reviewed
• [Journal Article] Control of floral stem cell activity in Arabidopsis (2019)
  • Author(s): Shang Erlei、Ito Toshiro、Sun Bo
  • Journal Title: Plant Signaling & Behavior Volume: 14 Issue: 11 Pages: 1659706-1659706
  • DOI: 10.1080/15592324.2019.1659706
  • Peer Reviewed
• [Journal Article] The Roles of Plant Hormones and Their Interactions with Regulatory Genes in Determining Meristem Activity (2019)
  • Author(s): Lee Ze Hong、Hirakawa Takeshi、Yamaguchi Nobutoshi、Ito Toshiro
  • Journal Title: International Journal of Molecular Sciences Volume: 20 Issue: 16 Pages: 4065-4065
  • DOI: 10.3390/ijms20164065
  • Peer Reviewed / Open Access
• [Journal Article] Abscisic acid‐dependent histone demethylation during postgermination growth arrest in Arabidopsis (2019)
  • Author(s): Wu Jinfeng、Ichihashi Yasunori、Suzuki Takamasa、Shibata Arisa、Shirasu Ken、Yamaguchi Nobutoshi、Ito Toshiro
  • Journal Title: Plant, Cell & Environment Volume: - Issue: 7 Pages: 2198-2214
  • DOI: 10.1111/pce.13547
  • Peer Reviewed / Open Access
• [Journal Article] Integration of transcriptional repression and Polycomb-mediated silencing of WUSCHEL in floral meristems (2019)
  • Author(s): Sun Bo、Zhou Yingying、Cai Jie、Shang Erlei、Yamaguchi Nobutoshi、Xiao Jun、Looi Liang-Sheng、Wee Wan-Yi、Gao Xiuying、Wagner Doris、Ito Toshiro
  • Journal Title: The Plant Cell Volume: - Issue: 7 Pages: 1488-1505
  • DOI: 10.1105/tpc.18.00450
  • NAID: 120006726651
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] When to stop: an update on molecular mechanisms of floral meristem termination (2019)
  • Author(s): Yifeng Xu, Nobutoshi Yamaguchi, Eng-Seng Gan, Toshiro Ito
  • Journal Title: Journal of Experimental Botany Volume: 70 Issue: 6 Pages: 1711-1718
  • DOI: 10.1093/jxb/erz048
  • Peer Reviewed
• [Journal Article] Histone demethylases control root elongation in response to stress-signaling hormone abscisic acid (2019)
  • Author(s): Wu Jinfeng、Yamaguchi Nobutoshi、Ito Toshiro
  • Journal Title: Plant Signaling & Behavior Volume: - Issue: 7 Pages: 1-4
  • DOI: 10.1080/15592324.2019.1604019
  • Peer Reviewed
• [Journal Article] Chromatin-mediated feed-forward auxin biosynthesis in floral meristem determinacy. (2018)
  • Author(s): Yamaguchi, N., Huang, J., Tatsumi, Y., Abe, M., Sugano, S. S., Kojima, M., Takebayashi, Y., Kiba, T., Yokoyama, R., Nishitani, K., Sakakibara, H., and Ito, T.
  • Journal Title: Nature Commun. Volume: 9 Issue: 1 Pages: 5290-5290
  • DOI: 10.1038/s41467-018-07763-0
  • NAID: 120006549567
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Meristem Termination and Organ Number Control in Early Stage of Arabidopsis Flower Development (2018)
  • Author(s): Xu Yifeng、Ito Toshiro
  • Journal Title: Journal of Cell Signaling Volume: 03 Issue: 03
  • DOI: 10.4172/2576-1471.1000186
  • Peer Reviewed
• [Journal Article] In Situ Proximity Ligation Assay to Detect the Interaction Between Plant Transcription Factors and Other Regulatory Proteins (2018)
  • Author(s): Yifeng Xu, Eng-Seng Gan, Toshiro Ito
  • Journal Title: Plant Transcription Factors.Methods in molecular biology (Clifton, N.J.) Volume: 1830 Pages: 325-335
  • DOI: 10.1007/978-1-4939-8657-6_19
  • ISBN: 9781493986569, 9781493986576
  • Peer Reviewed
• [Journal Article] Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis (2018)
  • Author(s): Ze Hong Lee, Nobutoshi Yamaguchi, Toshiro Ito
  • Journal Title: Plant Transcription Factors.Methods in molecular biology (Clifton, N.J.) Volume: 1830 Pages: 93-108
  • DOI: 10.1007/978-1-4939-8657-6_6
  • ISBN: 9781493986569, 9781493986576
  • Peer Reviewed
• [Journal Article] RGB-Color Intensiometric Indicators to Visualize Spatiotemporal Dynamics of ATP in Single Cells (2018)
  • Author(s): Arai Satoshi、Kriszt Rokus、Harada Kazuki、Looi Liang-Sheng、Matsuda Shogo、Wongso Devina、Suo Satoshi、Ishiura Shoichi、Tseng Yu-Hua、Raghunath Michael、Ito Toshiro、Tsuboi Takashi、Kitaguchi Tetsuya
  • Journal Title: Angewandte Chemie Volume: 130 Issue: 34 Pages: 11039-11044
  • DOI: 10.1002/ange.201804304
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] SUPERMAN regulates floral whorl boundaries through control of auxin biosynthesis. (2018)
  • Author(s): Xu, Y., Prunet, N., Gan, E-S., Wang, Y., Stewart, D., Wellmer, F., Huang, J., Yamaguchi, N., Tatsumi, Y., Kojima, M., Kiba, T., Sakakibara, H., Jack, T.P., Meyerowitz, E.M., Ito, T.
  • Journal Title: EMBO Journal Volume: - Issue: 11
  • DOI: 10.15252/embj.201797499
  • NAID: 120006478564
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 花幹細胞の増殖終結機構におけるオーキシン作用の二面性 (2020)
  • Author(s): 伊藤 寿朗
  • Organizer: 第61回日本植物生理学会大阪年会
• [Presentation] Epigenetic regulation in floral meristem determinacy (2019)
  • Author(s): 伊藤 寿朗
  • Organizer: 2019 Collaborative Research Meeting National Institute of Genetics
  • Int'l Joint Research
• [Presentation] Multistep termination of floral stem cell activities (2019)
  • Author(s): 伊藤 寿朗
  • Organizer: ICAR2019(The 30th International Conference on Arabidopsis Research)
  • Int'l Joint Research
• [Presentation] Auxin-mediated termination of floral stem cell activities (2019)
  • Author(s): 伊藤 寿朗
  • Organizer: Principles of pluripotent stem cells underlying plant vitality
  • Int'l Joint Research / Invited
• [Presentation] 花の儚さの分子機構 (2019)
  • Author(s): 伊藤 寿朗
  • Organizer: 2019年度 近畿植物学会講演会
  • Int'l Joint Research / Invited
• [Presentation] Multistep termination of floral stem cell activities (2019)
  • Author(s): 伊藤 寿朗
  • Organizer: Japan-Taiwan Plant Biology 2019 (JTPB2019)
  • Int'l Joint Research / Invited
• [Presentation] Spatio-temporal regulation of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: PSDB 10th Annual National Convention
  • Int'l Joint Research / Invited
• [Presentation] Memory and induced forgetting in plants (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: University of the Philippines-Diliman Ateneo de University 大学訪問セミナー
• [Presentation] 植物における記憶定着と能動的忘却システム (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: さきがけ懇親会
• [Presentation] Spatio-temporal regulation of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: Henan University大学訪問セミナー
  • Invited
• [Presentation] Multi-step termination of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: 福建農林大学さくらサイエンスプラン
• [Presentation] Auxin-mediated multistep termination of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: IPMB 2018
  • Int'l Joint Research / Invited
• [Presentation] 花幹細胞の時空間的な運命制御 (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: 京都大学形態統御学分科セミナー
  • Invited
• [Presentation] Auxin-mediated floral meristem determinacy control (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: 25th ICSPR 2018
  • Int'l Joint Research / Invited
• [Presentation] Auxin-mediated multistep termination of floral stem cell activities (2018)
  • Author(s): 伊藤 寿朗
  • Organizer: Meristem 2018
  • Int'l Joint Research / Invited