Relationship between the position of the spindle in aged oocytes and infertility

• Project/Area Number: 18K19347
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
• Research Institution: Waseda University
• Principal Investigator: Sato Masamitsu 早稲田大学, 理工学術院, 教授 (50447356)
• Project Period (FY): 2018-06-29 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 卵母細胞 / 不妊 / 微小管 / 減数分裂 / 染色体 / 細胞骨格 / 卵子 / 生殖医療

Outline of Final Research Achievements

ART (assisted reproductive technology) is recently becoming very important to reduce risks of chromosome abnormalities and improve the infertility problems. It is therefore essential to understand the mechanisms of infertility and chromosome trisomy from the viewpoint of basic science and to develop such knowledge into technologies for improvement of infertility. Here we tried to understand reasons for infertility from the viewpoint cell biology. Particularly we focused on the spindle in oocytes, which is often displaced in aged oocytes. Our research highlighted that such displacement of the spindle in unfertilized oocytes could serve as a guide to choose promising oocytes that have no sign of aging in terms of spindle positioning.

Academic Significance and Societal Importance of the Research Achievements

この紡錘体の位置の異常は、今後の生殖補助医療において、卵母細胞の劣化を示すひとつの指標となりうる。すなわち、不妊治療の現場において卵母細胞を採卵した際に、その中から劣化の兆候が見られず、出生の見込みがある卵母細胞を選択する際に、紡錘体の位置情報を劣化の指標として選別することで、妊娠の可能性を向上させることができるのではないかと想像される。このように、本研究の成果は、今後の臨床への応用の可能性が期待される。

Research Products

• [Journal Article] Cyst formation in proximal renal tubules caused by dysfunction of the microtubule minus-end regulator CAMSAP3 (2021)
  • Author(s): Mitsuhata Yuto、Abe Takaya、Misaki Kazuyo、Nakajima Yuna、Kiriya Keita、Kawasaki Miwa、Kiyonari Hiroshi、Takeichi Masatoshi、Toya Mika、Sato Masamitsu
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 5857-5857
  • DOI: 10.1038/s41598-021-85416-x
  • Peer Reviewed / Open Access
• [Journal Article] Simplification of nutritional conditions in transformation procedures for genome editing with the CRISPR/Cas9 system for fission yeast (2021)
  • Author(s): Li Seibun、Toya Mika、Sato Masamitsu
  • Journal Title: Gene Volume: 784 Pages: 145595-145595
  • DOI: 10.1016/j.gene.2021.145595
  • Peer Reviewed
• [Journal Article] Wake-up alarm: virtual time-lapse gene expression landscape illuminates mechanisms underlying dormancy breaking of germinating spores (2021)
  • Author(s): Tsuyuzaki Hayato、Ujiie Ryosuke、Sato Masamitsu
  • Journal Title: Current Genetics Volume: － Issue: 4 Pages: 519-534
  • DOI: 10.1007/s00294-021-01177-0
  • Peer Reviewed
• [Journal Article] Tell the Difference Between Mitosis and Meiosis: Interplay Between Chromosomes, Cytoskeleton, and Cell Cycle Regulation (2021)
  • Author(s): Sato Masamitsu、Kakui Yasutaka、Toya Mika
  • Journal Title: Frontiers in Cell and Developmental Biology Volume: 9 Pages: 1-1
  • DOI: 10.3389/fcell.2021.660322
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Time-lapse single-cell transcriptomics reveals modulation of histone H3 for dormancy breaking in fission yeast (2020)
  • Author(s): Hayato Tsuyuzaki, Masahito Hosokawa, Koji Arikawa, Takuya Yoda, Naoyuki Okada, Haruko Takeyama, Masamitsu Sato
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 1265-1265
  • DOI: 10.1038/s41467-020-15060-y
  • Peer Reviewed / Open Access
• [Journal Article] CLASP promotes microtubule bundling in metaphase spindle independently of Ase1/PRC1 in fission yeast (2019)
  • Author(s): Hirohisa Ebina, Liang Ji, Masamitsu Sato
  • Journal Title: Biology Open Volume: -
  • DOI: 10.1242/bio.045716
  • Peer Reviewed / Open Access
• [Journal Article] Kinetochore-mediated outward force promotes spindle pole separation in fission yeast (2019)
  • Author(s): Yutaka Shirasugi and Masamitsu Sato
  • Journal Title: Molecular Biology of the Cell Volume: 30 Issue: 22 Pages: 2802-2802
  • DOI: 10.1091/mbc.e19-07-0366
  • Peer Reviewed
• [Presentation] 子宮上皮におけるシグナル伝達兼転写活性化因子３（Stat3）はプロジェステロンシグナルを介して胚着床を制御する (2019)
  • Author(s): 白杉豊、工藤大和、並木貴文、影山敦子、寺川純平、大黒多希子、前澤創、長谷川嘉則、小原収、佐藤政充、伊藤潤哉、柏崎直巳
  • Organizer: 第112回日本繁殖生物学会大会
• [Presentation] 微小管が形成される場所・時間を連動して制御する分子メカニズム (2019)
  • Author(s): 佐藤政充、戸谷美夏
  • Organizer: 第92回日本生化学会大会
  • Invited
• [Presentation] 分裂酵母1細胞RNA-seq導入による胞子発芽時のトランスクリプトーム解析 (2019)
  • Author(s): 佐藤政充、露崎隼、岡田直幸
  • Organizer: 第37回染色体ワークショップ・第18回核ダイナミクス研究会
• [Presentation] Temporal Change of Single-Cell Transcriptome Reveals a “Wake-Up” Signal of Yeast Cells upon Nutrition Change (2018)
  • Author(s): Masamitsu Sato
  • Organizer: 2018 Core-to-core symposium Biotechnology Towards Next Generation Single Cell Analysis
  • Int'l Joint Research / Invited
• [Presentation] CDK-dependent nuclear accumulation of Alp7/TACC promotes the assembly of the radial array of microtubules in meiosis I. (2018)
  • Author(s): Yutaka Shirasugi, Masamitsu Sato
  • Organizer: 第70回細胞生物学会第51回日本発生生物学会合同大会
  • Int'l Joint Research
• [Presentation] Functional analysis of fission yeast CLASP in assembling pre-anaphase spindle (2018)
  • Author(s): Hirohisa Ebina, Liang Ji, Masamitsu Sato
  • Organizer: 第70回細胞生物学会第51回日本発生生物学会合同大会
  • Int'l Joint Research
• [Presentation] Functional linkage between the γ-tubulin ring complex and Alp7/TACC in microtubule nucleation (2018)
  • Author(s): Mana Katsuyama, Tomonari Sunaga, Masamitsu Sato
  • Organizer: 第70回細胞生物学会第51回日本発生生物学会合同大会
  • Int'l Joint Research
• [Presentation] Organization of microtubules in small intestinal crypt cells (2018)
  • Author(s): Yuto Mitsuhata, Mika Toya, Masatoshi Takeichi, Masamitsu Sato
  • Organizer: 第70回細胞生物学会第51回日本発生生物学会合同大会
  • Int'l Joint Research
• [Remarks] 佐藤研究室website
  • URL: http://www.sato.biomed.sci.waseda.ac.jp/
• [Remarks] 早稲田大学生命医科学科 佐藤研究室
  • URL: http://www.sato.biomed.sci.waseda.ac.jp/