| Project/Area Number |
18K19348
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2018-06-29 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 生物時計 / 概月時計 / 光受容 / 月光応答 / 生物リズム / 同調産卵 |
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| Outline of Final Research Achievements |
Fish such as puffer fish and spinefoot show a spawning behavior depending on the moon phase. In photobiology and chronobiology, the mechanism of the moonlight response is still unknown. In order to elucidate this molecular mechanism, it is important to establish a cell line that responds to moonlight and examine the presence or absence of moonlight responsiveness and circalunar rhthm. Therefore, in this study, we constructed an artificial moonlight device that reproduces the moonlight with complex changes in brightness and light period. Using this device, we examined the moonlight responsiveness of circadian clock genes in cultured pufferfish cells and individual fish. It was clarified that the pufferfish eye-derived Fugu Eye cells could be used as a model of the moonlight response mechanism.
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| Academic Significance and Societal Importance of the Research Achievements |
この20年の間に概日時計の仕組みが次々と解明された。それに比べて、フグやアイゴの産卵に見られる２週間あるいは１月周期性の分子機構は不明である。これを明らかにするためには、月光に応答する細胞を用いて、長期間に渡り遺伝子発現変動を調べる系の確立が重要であった。本研究では、Fugu Eye細胞が、その第一歩となるモデルとして利用できる可能性を示した。本研究の成果は、将来的にはフグの産卵を制御し、より効率的な養殖や育種につながる可能性がある。
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