Toward the understanding of land plant evolution, through the analyses of zygnematophycean algae

• Project/Area Number: 18K19365
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 45:Biology at organismal to population levels and anthropology, and related fields
• Research Institution: Japan Women's University
• Principal Investigator: SEKIMOTO Hiroyuki 日本女子大学, 理学部, 教授 (20281652)
• Co-Investigator(Kenkyū-buntansha): 池谷 仁里 神戸大学, 理学研究科, 研究員 (30531579) ; 西山 智明 金沢大学, 疾患モデル総合研究センター, 助教 (50390688)
• Project Period (FY): 2018-06-29 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: ミカヅキモ / アオミドロ / 転写因子 / ヒメミカヅキモ / ゲノム / シャジクモ / 多細胞化 / ホシミドロ目

Outline of Final Research Achievements

In this study, we focused on two streptophyte algae, Spirogyra parvula (filamentous) and the Closterium peracerosum-strigosum-littorale complex (unicellular) to reveal the factors for the land plant evolution. For this purpose, we tried to obtain whole genome sequences of these organisms and establish techniques for reverse genetics on Spirogyra parvula. We also tried to discover genes encoding a transcription factor acting for maintaining the multicellular body and differentiation of specific functions.

Academic Significance and Societal Importance of the Research Achievements

本研究では、アオミドロでは逆遺伝学的手法の確立には至らなかったが、ホシミドロ目の糸状性種と単細胞種に注目し、両者のゲノム解読および転写因子遺伝子同定を行ったことは、植物進化研究という学術的意義のみならず、植物がどのように進化してきたかを社会に伝えるための第一歩としても非常に高い意義がある。

Research Products

• [Journal Article] A divergent RWP-RK transcription factor determines mating type in heterothallic Closterium (2023)
  • Author(s): Sekimoto, H., Komiya, A., Tsuyuki, N., Kawai, J., Kanda, N., Ootsuki, R., Suzuki, Y., Toyoda, A., Fujiyama, A., Kasahara, M., Abe, J., Tsuchikane, Y., Nishiyama, T.
  • Journal Title: New Phytologist Volume: 237 Issue: 5 Pages: 1636-1651
  • DOI: 10.1111/nph.18662
  • Peer Reviewed / Open Access
• [Journal Article] Zygnematophycean algae: Possible models for cellular and evolutionary biology (2022)
  • Author(s): Ohtaka Kinuka、Sekimoto Hiroyuki
  • Journal Title: Seminars in Cell & Developmental Biology Volume: - Pages: 59-68
  • DOI: 10.1016/j.semcdb.2022.03.042
  • Peer Reviewed
• [Journal Article] Three sex phenotypes in a haploid algal species give insights into the evolutionary transition to a self-compatible mating system (2021)
  • Author(s): Kohei Takahashi, Hiroko Kawai-Toyooka, Ryo Ootsuki, Takashi Hamaji, Yuki Tsuchikane, Hiroyuki Sekimoto, Tetsuya Higashiyama, Hisayoshi Nozaki
  • Journal Title: Evolution Volume: 75 Issue: 11 Pages: 2984-2993
  • DOI: 10.1111/evo.14306
  • Peer Reviewed / Open Access
• [Journal Article] Highly efficient transformation of the model zygnematophycean alga <i>Closterium peracerosum‐strigosum‐littorale</i> complex by square‐pulse electroporation (2021)
  • Author(s): Kawai Junko、Kanazawa Manaki、Suzuki Rie、Kikuchi Nanako、Hayakawa Yasuhiko、Sekimoto Hiroyuki
  • Journal Title: New Phytologist Volume: 233 Issue: 1 Pages: 569-578
  • DOI: 10.1111/nph.17763
  • Peer Reviewed
• [Journal Article] Anthoceros genomes illuminate the origin of land plants and the unique biology of hornworts (2020)
  • Author(s): Li FW, Nishiyama T, et al.
  • Journal Title: Nat. Plants Volume: 6 Issue: 3 Pages: 259-272
  • DOI: 10.1038/s41477-020-0618-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Expression of genes from paternal alleles in rice zygotes and involvement of OsASGR-BBML1 in initiation of zygotic development (2019)
  • Author(s): Rahman, H., Toda, E., Kobayashi, M., Kudo, T., Koshimizu, S., Takahara, M., Iwami, M., Watanabe, Y., Sekimoto, H., Yano, K., Okamoto, T.
  • Journal Title: Plant Cell Physiol. Volume: 60 Issue: 4 Pages: 725-737
  • DOI: 10.1093/pcp/pcz030
  • Peer Reviewed / Open Access
• [Journal Article] The genus Closterium, a new model organism to study sexual reproduction in streptophytes (2019)
  • Author(s): Tsuchikane, Y., Sekimoto, H.
  • Journal Title: New Phytol. Volume: 221 Issue: 1 Pages: 99-104
  • DOI: 10.1111/nph.15334
  • Peer Reviewed
• [Journal Article] Identification of a new mating group and reproductive isolation in the Closterium peracerosum-strigosum-littorale complex (2018)
  • Author(s): Tsuchikane, Y., Kobayashi, H., Kato, M., Watanabe, J., Wu, J.-T., Sekimoto, H.
  • Journal Title: J. Plant Res. Volume: 131 Issue: 5 Pages: 735-746
  • DOI: 10.1007/s10265-018-1043-8
  • NAID: 40021652422
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants (2018)
  • Author(s): Higo, A., Kawashima, T., Borg, M., Zhao, M., Lopez-Vidriero, I., Sakayama, H., et al. (他18名)
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 5283-5283
  • DOI: 10.1038/s41467-018-07728-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] アオミドロ属Spirogyra parvulaのゲノム解析から探る接合藻類単細胞化と遺伝子喪失 (2023)
  • Author(s): 西山智明、池谷 仁里、高野 智之、坂山 英俊、関本 弘之
  • Organizer: 植物分類学会第22回大会
• [Presentation] 多細胞接合藻アオミドロ属Spirogyra parvulaゲノムの解読と単細胞性接合藻および陸上植物との比較解析 (2023)
  • Author(s): 西山智明、池谷 仁里、高野 智之、坂山 英俊、関本 弘之
  • Organizer: 日本植物生理学会第64回年会
• [Presentation] ヒメミカヅキモにおけるコピー数変異がもたらした顕著なゲノムサイズ多型と遺伝子発現への影響 (2022)
  • Author(s): 川口也和子、土金勇樹、田中啓介、太治輝昭、豊田敦、西山智明、関本弘之、土松隆志
  • Organizer: 日本進化学会第24回大会
• [Presentation] 多細胞接合藻類アオミドロのゲノム解読 (2022)
  • Author(s): 西山智明、池谷仁里、高野智之、坂山英俊、関本弘之
  • Organizer: 日本植物学会第86回大会
• [Presentation] 異なる性表現タイプのボルボックス間比較ゲノム解析からホモタリック化に伴う性決定領域の進化を探る (2022)
  • Author(s): 山本荷葉子、松崎令、Wuttipong Mahakham、Wirawan Heman、関本弘之、河地正伸，水口洋平，豊田 敦，野崎久義
  • Organizer: 日本植物学会第86回大会
• [Presentation] ヒメミカヅキモBELL1遺伝子の有性生殖過程における機能解析 (2022)
  • Author(s): 專田梨瑛子，加来 卓也，川井 絢子，西山 智明，関本 弘之，榊原 恵子
  • Organizer: 日本植物学会第86回大会
• [Presentation] Male sex-determining region retained after transition from heterothallism to homothallism in a Thai strain of Volvox africanus (2022)
  • Author(s): Yamamoto K., Matsuzaki, R., Mahakham, W., Heman, W., Sekimoto, H., Kawachi, M., Minakuchi, Y., Toyoda, A., Nozaki, H.
  • Organizer: Volvox 2022
  • Int'l Joint Research
• [Presentation] A divergent RWP-RK transcription factor determines mating type in heterothallic Closterium (2022)
  • Author(s): Sekimoto, H., Tsuyuki, N., Komiya, A., Nishiyama, T.
  • Organizer: EMBO Workshop _An integrated view of early land plant evolution
  • Int'l Joint Research
• [Presentation] ヒメミカヅキモにおける顕著なゲノムサイズ多型の進化的起源と遺伝子発現パターンへの影響 (2022)
  • Author(s): 川口也和子、土金勇樹、田中啓介、太治輝昭、豊田敦、西山智明、関本弘之、土松隆志
  • Organizer: 日本生態学会第69回大会
• [Presentation] ヒメミカヅキモ種内にみられる大規模なゲノムサイズ変異の進化的起源 (2021)
  • Author(s): 川口也和子、土金勇樹、田中啓介、太治輝昭、豊田敦、西山智明、関本弘之、土松隆志
  • Organizer: 日本進化学会第23回大会
• [Presentation] 蛍光タンパク質融合CenH3発現によるヒメミカヅキモの染色体解析とゲノム進化 (2021)
  • Author(s): 渡辺実咲、村田隆、堤元佐、大友康平、根本知己、川口也和子、土松隆志、西山智明、土金勇樹、関本弘之
  • Organizer: 日本植物学会第85回大会
• [Presentation] ヒメミカヅキモの有性生殖進行に機能する受容体型キナーゼ遺伝子の解析 (2021)
  • Author(s): 山口里帆子、西山智明、神田奈保、今詩織、関本弘之
  • Organizer: 日本植物学会第85回大会
• [Presentation] ヒメミカヅキモにおける蛍光標識CenH3タンパク質の発現による染色体可視化 (2021)
  • Author(s): 渡辺実咲, 村田隆, 堤元佐, 大友康平, 根本知己, 西山智明, 土金勇樹, 関本弘之
  • Organizer: 日本藻類学会第45回大会
• [Presentation] 全ゲノムデータからみたヒメミカヅキモの自殖系統におけるホメオログ間組み換え (2020)
  • Author(s): 川口也和子，土金勇樹，田中啓介，太治輝昭，豊田敦，西山智明，関本弘之，土松隆志
  • Organizer: 日本植物学会第84回大会
• [Presentation] ヒメミカヅキモのPR-IPは、性フェロモンであるが有性生殖に必須ではない (2020)
  • Author(s): 山口里帆子、福島早貴、今詩織、神田奈保、関本弘之
  • Organizer: 日本植物学会第84回大会
• [Presentation] The mechanism of mating type determination in heterothallic strains of Closterium peracerosum-strigosum-littorale complex, a new model organism to study sexual reproduction in streptophytes (2020)
  • Author(s): Hiroyuki Sekimoto, Natsumi Tsuyuki, Ayumi Komiya, Tomoaki Nishiyama
  • Organizer: 第43回日本分子生物学会年会
  • Invited
• [Presentation] 接合藻ヒメミカヅキモにおける生殖様式の平行進化:全ゲノムデータから探る (2019)
  • Author(s): 川口也和子、土金勇樹、田中啓介、太治輝昭、豊田敦、西山智明、関本弘之、土松隆志
  • Organizer: 日本進化学会第21回大会
• [Presentation] アストレフォメネの多細胞形質進化の解明に向けた新規ゲノムと形質転換の確立 (2019)
  • Author(s): 山下翔大、数口敦紀、山本荷葉子、松崎令、野口英樹、水口洋平、豊田敦、河地正伸、廣野雅文、関本弘之、野崎久義
  • Organizer: 日本植物形態学会第31回大会
• [Presentation] ヒメミカヅキモの遺伝子アノテーションとCpMinus1形質転換体の比較トランスクリプトーム解析 (2019)
  • Author(s): 関本弘之、露木奈津美、小宮あゆみ、西山智明
  • Organizer: 日本植物学会第83回大会
• [Presentation] Unveiling the molecular bases involved in another evolution of multicellular complexity in Astrephomene gubernaculifera (2019)
  • Author(s): Yamashita, S., Suguchi, A., Yamamoto, K., Matsuzaki, R., Kawachi, M., Noguchi, H., Minakuchi, Y., Toyoda, A., Hirono, M., Sekimoto, H., Nozaki, H.
  • Organizer: The 5th International Volvox Conference
  • Int'l Joint Research
• [Presentation] Three sexes in the anisogamous volvocine alga Pleodorina starrii (2019)
  • Author(s): Takahashi, K., Yamashita, S., Yamamoto, K., Kawai-Toyooka, H., Tsuchikane, Y., Sekimoto, H., Nozaki, H.
  • Organizer: The 5th International Volvox Conference
  • Int'l Joint Research
• [Presentation] The mating type determination in heterothallic strains of Closterium peracerosum-strigosum-littorale complex, a new model organism to study sexual reproduction in charophycean algae (2019)
  • Author(s): Sekimoto, H.
  • Organizer: The 5th International Volvox Conference
  • Int'l Joint Research
• [Presentation] The mechanism of mating type determination in heterothallic strains of Closterium peracerosum-strigosum-littorale complex, a new model organism to study sexual reproduction in streptophytes (2019)
  • Author(s): Sekimoto, H., Tsuyuki, N., Komiya, A., Nishiyama, T.
  • Organizer: The SEB satellite meeting "Algal model systems on the rise: understanding and exploiting the algae to land plant transition"
  • Int'l Joint Research / Invited
• [Presentation] ネパール産ミカヅキモ属の多様性と新規交配群Hの発見 (2019)
  • Author(s): 小林弘佳、川口也和子、Mangal Man Shakya、土松隆志、関本弘之、土金勇樹
  • Organizer: 日本植物分類学会18回大会
• [Presentation] CRISPR/Cas9-based knockouts reveal the action mechanism of the sex pheromone PR-IP in unicellular zygnematophycean alga, Closterium peracerosum-strigosum-littorale complex (2018)
  • Author(s): Sekimoto, H.
  • Organizer: EMBO Workshop _New shores in land plant evolution
  • Int'l Joint Research
• [Presentation] Auxin response in conjugating green algae (Zygnematophyceae) (2018)
  • Author(s): Roman Skokan, Stanislav Vosolsob_, Junko Kawai, Hiroyuki Sekimoto, Henrik Buschmann, Jan Petr__ek
  • Organizer: EMBO Workshop _New shores in land plant evolution
  • Int'l Joint Research
• [Presentation] ヒメミカヅキモの有性生殖進行に関わるRWP-RK遺伝子の解析 (2018)
  • Author(s): 平田優香、土金勇樹、西山智明、関本弘之
  • Organizer: 日本植物学会第82回大会
• [Presentation] ヒメミカヅキモのCpMinus1形質転換体の比較トランスクリプトーム解析 (2018)
  • Author(s): 露木奈津美、小宮あゆみ、大槻涼、西山智明、関本弘之
  • Organizer: 日本植物学会第82回大会
• [Presentation] 接合藻ヒメミカヅキモ種内系統間にみられるゲノム構造の変異：生殖様式の進化との関連 (2018)
  • Author(s): 川口也和子、土金勇樹、西山智明、関本弘之、土松隆志
  • Organizer: 日本植物学会第82回大会
• [Presentation] ヒメミカヅキモの性フェロモンPR-IP Inducerによる生殖隔離障壁 (2018)
  • Author(s): 土金勇樹、小林弘佳、吉田睦未、関本弘之
  • Organizer: 日本植物学会第82回大会
• [Presentation] シャジクモ藻類ヒメミカヅキモのモデル植物化 (2018)
  • Author(s): 関本弘之
  • Organizer: 日本植物学会第82回大会
  • Invited
• [Remarks] 日本女子大学理学部物質生物科学科 関本研究室
  • URL: http://mcm-www.jwu.ac.jp/~sekimoto/Site/Home.html