• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Toward the understanding of land plant evolution, through the analyses of zygnematophycean algae

Research Project

Project/Area Number 18K19365
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 45:Biology at organismal to population levels and anthropology, and related fields
Research InstitutionJapan Women's University

Principal Investigator

SEKIMOTO Hiroyuki  日本女子大学, 理学部, 教授 (20281652)

Co-Investigator(Kenkyū-buntansha) 池谷 仁里  神戸大学, 理学研究科, 研究員 (30531579)
西山 智明  金沢大学, 疾患モデル総合研究センター, 助教 (50390688)
Project Period (FY) 2018-06-29 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsミカヅキモ / アオミドロ / 転写因子 / ヒメミカヅキモ / ゲノム / シャジクモ / 多細胞化 / ホシミドロ目
Outline of Final Research Achievements

In this study, we focused on two streptophyte algae, Spirogyra parvula (filamentous) and the Closterium peracerosum-strigosum-littorale complex (unicellular) to reveal the factors for the land plant evolution. For this purpose, we tried to obtain whole genome sequences of these organisms and establish techniques for reverse genetics on Spirogyra parvula. We also tried to discover genes encoding a transcription factor acting for maintaining the multicellular body and differentiation of specific functions.

Academic Significance and Societal Importance of the Research Achievements

本研究では、アオミドロでは逆遺伝学的手法の確立には至らなかったが、ホシミドロ目の糸状性種と単細胞種に注目し、両者のゲノム解読および転写因子遺伝子同定を行ったことは、植物進化研究という学術的意義のみならず、植物がどのように進化してきたかを社会に伝えるための第一歩としても非常に高い意義がある。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (41 results)

All 2023 2022 2021 2020 2019 2018 Other

All Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 5 results) Presentation (31 results) (of which Int'l Joint Research: 8 results,  Invited: 3 results) Remarks (1 results)

  • [Journal Article] A divergent RWP-RK transcription factor determines mating type in heterothallic Closterium2023

    • Author(s)
      Sekimoto, H., Komiya, A., Tsuyuki, N., Kawai, J., Kanda, N., Ootsuki, R., Suzuki, Y., Toyoda, A., Fujiyama, A., Kasahara, M., Abe, J., Tsuchikane, Y., Nishiyama, T.
    • Journal Title

      New Phytologist

      Volume: 237 Issue: 5 Pages: 1636-1651

    • DOI

      10.1111/nph.18662

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Zygnematophycean algae: Possible models for cellular and evolutionary biology2022

    • Author(s)
      Ohtaka Kinuka、Sekimoto Hiroyuki
    • Journal Title

      Seminars in Cell & Developmental Biology

      Volume: - Pages: 59-68

    • DOI

      10.1016/j.semcdb.2022.03.042

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three sex phenotypes in a haploid algal species give insights into the evolutionary transition to a self-compatible mating system2021

    • Author(s)
      Kohei Takahashi, Hiroko Kawai-Toyooka, Ryo Ootsuki, Takashi Hamaji, Yuki Tsuchikane, Hiroyuki Sekimoto, Tetsuya Higashiyama, Hisayoshi Nozaki
    • Journal Title

      Evolution

      Volume: 75 Issue: 11 Pages: 2984-2993

    • DOI

      10.1111/evo.14306

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highly efficient transformation of the model zygnematophycean alga <i>Closterium peracerosum‐strigosum‐littorale</i> complex by square‐pulse electroporation2021

    • Author(s)
      Kawai Junko、Kanazawa Manaki、Suzuki Rie、Kikuchi Nanako、Hayakawa Yasuhiko、Sekimoto Hiroyuki
    • Journal Title

      New Phytologist

      Volume: 233 Issue: 1 Pages: 569-578

    • DOI

      10.1111/nph.17763

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Anthoceros genomes illuminate the origin of land plants and the unique biology of hornworts2020

    • Author(s)
      Li FW, Nishiyama T, et al.
    • Journal Title

      Nat. Plants

      Volume: 6 Issue: 3 Pages: 259-272

    • DOI

      10.1038/s41477-020-0618-2

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Expression of genes from paternal alleles in rice zygotes and involvement of OsASGR-BBML1 in initiation of zygotic development2019

    • Author(s)
      Rahman, H., Toda, E., Kobayashi, M., Kudo, T., Koshimizu, S., Takahara, M., Iwami, M., Watanabe, Y., Sekimoto, H., Yano, K., Okamoto, T.
    • Journal Title

      Plant Cell Physiol.

      Volume: 60 Issue: 4 Pages: 725-737

    • DOI

      10.1093/pcp/pcz030

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The genus Closterium, a new model organism to study sexual reproduction in streptophytes2019

    • Author(s)
      Tsuchikane, Y., Sekimoto, H.
    • Journal Title

      New Phytol.

      Volume: 221 Issue: 1 Pages: 99-104

    • DOI

      10.1111/nph.15334

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of a new mating group and reproductive isolation in the Closterium peracerosum-strigosum-littorale complex2018

    • Author(s)
      Tsuchikane, Y., Kobayashi, H., Kato, M., Watanabe, J., Wu, J.-T., Sekimoto, H.
    • Journal Title

      J. Plant Res.

      Volume: 131 Issue: 5 Pages: 735-746

    • DOI

      10.1007/s10265-018-1043-8

    • NAID

      40021652422

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants2018

    • Author(s)
      Higo, A., Kawashima, T., Borg, M., Zhao, M., Lopez-Vidriero, I., Sakayama, H., et al. (他18名)
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 5283-5283

    • DOI

      10.1038/s41467-018-07728-3

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] アオミドロ属Spirogyra parvulaのゲノム解析から探る接合藻類単細胞化と遺伝子喪失2023

    • Author(s)
      西山智明、池谷 仁里、高野 智之、坂山 英俊、関本 弘之
    • Organizer
      植物分類学会第22回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 多細胞接合藻アオミドロ属Spirogyra parvulaゲノムの解読と単細胞性接合藻および陸上植物との比較解析2023

    • Author(s)
      西山智明、池谷 仁里、高野 智之、坂山 英俊、関本 弘之
    • Organizer
      日本植物生理学会第64回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ヒメミカヅキモにおけるコピー数変異がもたらした顕著なゲノムサイズ多型と遺伝子発現への影響2022

    • Author(s)
      川口也和子、土金勇樹、田中啓介、太治輝昭、豊田敦、西山智明、関本弘之、土松隆志
    • Organizer
      日本進化学会第24回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 多細胞接合藻類アオミドロのゲノム解読2022

    • Author(s)
      西山智明、池谷仁里、高野智之、坂山英俊、関本弘之
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 異なる性表現タイプのボルボックス間比較ゲノム解析からホモタリック化に伴う性決定領域の進化を探る2022

    • Author(s)
      山本荷葉子、松崎令、Wuttipong Mahakham、Wirawan Heman、関本弘之、河地正伸,水口洋平,豊田 敦,野崎久義
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ヒメミカヅキモBELL1遺伝子の有性生殖過程における機能解析2022

    • Author(s)
      專田梨瑛子,加来 卓也,川井 絢子,西山 智明,関本 弘之,榊原 恵子
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Male sex-determining region retained after transition from heterothallism to homothallism in a Thai strain of Volvox africanus2022

    • Author(s)
      Yamamoto K., Matsuzaki, R., Mahakham, W., Heman, W., Sekimoto, H., Kawachi, M., Minakuchi, Y., Toyoda, A., Nozaki, H.
    • Organizer
      Volvox 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A divergent RWP-RK transcription factor determines mating type in heterothallic Closterium2022

    • Author(s)
      Sekimoto, H., Tsuyuki, N., Komiya, A., Nishiyama, T.
    • Organizer
      EMBO Workshop _An integrated view of early land plant evolution
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒメミカヅキモにおける顕著なゲノムサイズ多型の進化的起源と遺伝子発現パターンへの影響2022

    • Author(s)
      川口也和子、土金勇樹、田中啓介、太治輝昭、豊田敦、西山智明、関本弘之、土松隆志
    • Organizer
      日本生態学会第69回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヒメミカヅキモ種内にみられる大規模なゲノムサイズ変異の進化的起源2021

    • Author(s)
      川口也和子、土金勇樹、田中啓介、太治輝昭、豊田敦、西山智明、関本弘之、土松隆志
    • Organizer
      日本進化学会第23回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 蛍光タンパク質融合CenH3発現によるヒメミカヅキモの染色体解析とゲノム進化2021

    • Author(s)
      渡辺実咲、村田隆、堤元佐、大友康平、根本知己、川口也和子、土松隆志、西山智明、土金勇樹、関本弘之
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヒメミカヅキモの有性生殖進行に機能する受容体型キナーゼ遺伝子の解析2021

    • Author(s)
      山口里帆子、西山智明、神田奈保、今詩織、関本弘之
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヒメミカヅキモにおける蛍光標識CenH3タンパク質の発現による染色体可視化2021

    • Author(s)
      渡辺実咲, 村田隆, 堤元佐, 大友康平, 根本知己, 西山智明, 土金勇樹, 関本弘之
    • Organizer
      日本藻類学会第45回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 全ゲノムデータからみたヒメミカヅキモの自殖系統におけるホメオログ間組み換え2020

    • Author(s)
      川口也和子,土金勇樹,田中啓介,太治輝昭,豊田敦,西山智明,関本弘之,土松隆志
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ヒメミカヅキモのPR-IPは、性フェロモンであるが有性生殖に必須ではない2020

    • Author(s)
      山口里帆子、福島早貴、今詩織、神田奈保、関本弘之
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] The mechanism of mating type determination in heterothallic strains of Closterium peracerosum-strigosum-littorale complex, a new model organism to study sexual reproduction in streptophytes2020

    • Author(s)
      Hiroyuki Sekimoto, Natsumi Tsuyuki, Ayumi Komiya, Tomoaki Nishiyama
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 接合藻ヒメミカヅキモにおける生殖様式の平行進化:全ゲノムデータから探る2019

    • Author(s)
      川口也和子、土金勇樹、田中啓介、太治輝昭、豊田敦、西山智明、関本弘之、土松隆志
    • Organizer
      日本進化学会第21回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] アストレフォメネの多細胞形質進化の解明に向けた新規ゲノムと形質転換の確立2019

    • Author(s)
      山下翔大、数口敦紀、山本荷葉子、松崎令、野口英樹、水口洋平、豊田敦、河地正伸、廣野雅文、関本弘之、野崎久義
    • Organizer
      日本植物形態学会第31回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ヒメミカヅキモの遺伝子アノテーションとCpMinus1形質転換体の比較トランスクリプトーム解析2019

    • Author(s)
      関本弘之、露木奈津美、小宮あゆみ、西山智明
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Unveiling the molecular bases involved in another evolution of multicellular complexity in Astrephomene gubernaculifera2019

    • Author(s)
      Yamashita, S., Suguchi, A., Yamamoto, K., Matsuzaki, R., Kawachi, M., Noguchi, H., Minakuchi, Y., Toyoda, A., Hirono, M., Sekimoto, H., Nozaki, H.
    • Organizer
      The 5th International Volvox Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Three sexes in the anisogamous volvocine alga Pleodorina starrii2019

    • Author(s)
      Takahashi, K., Yamashita, S., Yamamoto, K., Kawai-Toyooka, H., Tsuchikane, Y., Sekimoto, H., Nozaki, H.
    • Organizer
      The 5th International Volvox Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] The mating type determination in heterothallic strains of Closterium peracerosum-strigosum-littorale complex, a new model organism to study sexual reproduction in charophycean algae2019

    • Author(s)
      Sekimoto, H.
    • Organizer
      The 5th International Volvox Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] The mechanism of mating type determination in heterothallic strains of Closterium peracerosum-strigosum-littorale complex, a new model organism to study sexual reproduction in streptophytes2019

    • Author(s)
      Sekimoto, H., Tsuyuki, N., Komiya, A., Nishiyama, T.
    • Organizer
      The SEB satellite meeting "Algal model systems on the rise: understanding and exploiting the algae to land plant transition"
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ネパール産ミカヅキモ属の多様性と新規交配群Hの発見2019

    • Author(s)
      小林弘佳、川口也和子、Mangal Man Shakya、土松隆志、関本弘之、土金勇樹
    • Organizer
      日本植物分類学会18回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] CRISPR/Cas9-based knockouts reveal the action mechanism of the sex pheromone PR-IP in unicellular zygnematophycean alga, Closterium peracerosum-strigosum-littorale complex2018

    • Author(s)
      Sekimoto, H.
    • Organizer
      EMBO Workshop _New shores in land plant evolution
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Auxin response in conjugating green algae (Zygnematophyceae)2018

    • Author(s)
      Roman Skokan, Stanislav Vosolsob_, Junko Kawai, Hiroyuki Sekimoto, Henrik Buschmann, Jan Petr__ek
    • Organizer
      EMBO Workshop _New shores in land plant evolution
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒメミカヅキモの有性生殖進行に関わるRWP-RK遺伝子の解析2018

    • Author(s)
      平田優香、土金勇樹、西山智明、関本弘之
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ヒメミカヅキモのCpMinus1形質転換体の比較トランスクリプトーム解析2018

    • Author(s)
      露木奈津美、小宮あゆみ、大槻涼、西山智明、関本弘之
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 接合藻ヒメミカヅキモ種内系統間にみられるゲノム構造の変異:生殖様式の進化との関連2018

    • Author(s)
      川口也和子、土金勇樹、西山智明、関本弘之、土松隆志
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ヒメミカヅキモの性フェロモンPR-IP Inducerによる生殖隔離障壁2018

    • Author(s)
      土金勇樹、小林弘佳、吉田睦未、関本弘之
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] シャジクモ藻類ヒメミカヅキモのモデル植物化2018

    • Author(s)
      関本弘之
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Remarks] 日本女子大学理学部物質生物科学科 関本研究室

    • URL

      http://mcm-www.jwu.ac.jp/~sekimoto/Site/Home.html

    • Related Report
      2019 Research-status Report

URL: 

Published: 2018-07-25   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi