• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of 3D pathological analysis of cerebral small vessel disease

Research Project

Project/Area Number 18K19373
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 46:Neuroscience and related fields
Research InstitutionNiigata University

Principal Investigator

Tainaka Kazuki  新潟大学, 脳研究所, 教授 (80506113)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords脳小血管病 / 組織透明化 / 3Dイメージング / 神経病理
Outline of Final Research Achievements

In this study, we developed a novel tissue clearing protocol for postmortem human brain tissue, and whole-mount immunohistochemistry for visualizing vasculature-related molecules to achieve 3D imaging of cerebral small vessel disease. We obtained an efficient delipidation chemicals for human white matter, and succeeded to suppress strong autofluorescence derived from human brain such as lipofuscin. According to a series of these techniques, we analyzed denaturation of capillary, functional disorders of astrocyte, and white matter lesion in clinical tissue samples from such as CADASIL.

Academic Significance and Societal Importance of the Research Achievements

脳小血管病は脳梗塞の再発、認知機能、歩行障害などと関連することが知られており、臨床の現場では病態の解明および予防策の検討が急務とされている。しかしながら、毛細血管を構成する細胞種の同定や周囲のグリア細胞との関係性、並びに血液脳関門の機能的破綻などを立体的かつ高解像度に可視化する研究手法が確立されていなかったため、治療戦略に繋がる病理所見を得ることが難しかった。本研究により、ヒト脳病理標本の高度な透明化技術と共に血管やその他の関連因子を立体的に可視化する汎用性の高い免疫染色プロトコールが得られた。従って、脳小血管病の3D病態解析を推進する上で必要なシーズとなる萌芽的技術が得られた。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (17 results)

All 2020 2019 2018

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (13 results) (of which Int'l Joint Research: 4 results,  Invited: 8 results) Book (2 results)

  • [Journal Article] Rapid chemical clearing of white matter in the post-mortem human brain by 1,2-hexanediol delipidation.2019

    • Author(s)
      Inoue M, Saito R, Kakita A, Tainaka K.
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 29 Issue: 15 Pages: 1886-1890

    • DOI

      10.1016/j.bmcl.2019.05.049

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents.2018

    • Author(s)
      Tainaka K, Murakami TC, Susaki EA, Shimizu C, Saito R, Takahashi K, Hayashi-Takagi A, Sekiya H, Arima Y, Nojima S, Ikemura M, Ushiku T, Shimizu Y, Murakami M, Tanaka KF, Iino M, Kasai H, Sasaoka T, Kobayashi K, Miyazono K, Morii E, Isa T, Fukayama M, Kakita A, Ueda HR.
    • Journal Title

      Cell Rep.

      Volume: 24 Issue: 8 Pages: 2196-2210

    • DOI

      10.1016/j.celrep.2018.07.056

    • NAID

      120006542018

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] CUBIC: Whole-brain/body imaging with single-cell resolution using hydrophilic chemical cocktails2019

    • Author(s)
      Kazuki Tainaka
    • Organizer
      International Symposium of Biofunctional Chemistry
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] CUBIC:生体組織透明化による包括的3Dイメージング技術2019

    • Author(s)
      田井中一貴
    • Organizer
      新潟医学会特別講演
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] CUBIC: 組織透明化技術による3Dイメージング2019

    • Author(s)
      田井中一貴
    • Organizer
      第60回新潟生化学懇話会プログラム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 3D 神経病理学に向けたホールマウント染色手法の開発2019

    • Author(s)
      田井中 一貴・齋藤 理恵・井上 雅文・柿田 明美
    • Organizer
      第60回日本神経病理学会総会学術研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Comprehensive 3D Imaging by Tissue Clearing Technique CUBIC2019

    • Author(s)
      Kazuki Tainaka
    • Organizer
      Neuro2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 組織透明化による3D神経病理学2019

    • Author(s)
      田井中一貴
    • Organizer
      第16回 日本病理学会カンファレンス
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] CUBIC: Whole-organ, whole-body imaging with single-cell resolution using chemical cocktails2019

    • Author(s)
      Kazuki Tainaka
    • Organizer
      リエゾンラボ炎症シンポジウムin北海道大学
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 有機化学が拓く次世代3D組織化学2019

    • Author(s)
      田井中一貴
    • Organizer
      第5回 北海道大学部局横断シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] CUBIC: Whole-brain/body imaging with single-cell resolution using hydrophilic chemical cocktails2019

    • Author(s)
      Kazuki Tainaka
    • Organizer
      BRI The 9th international symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 組織透明化技術CUBICによる3D神経病理学2018

    • Author(s)
      田井中一貴
    • Organizer
      日本ケミカルバイオロジー学会 第13回年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 組織透明化技術CUBICによる3D神経病理学2018

    • Author(s)
      田井中一貴
    • Organizer
      第107回日本病理学会総会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 水溶性試薬による組織透明化の化学2018

    • Author(s)
      田井中一貴
    • Organizer
      第12回バイオ関連化学シンポジウム
    • Related Report
      2018 Research-status Report
  • [Presentation] Development of 3D neuropathology based on tissue clearing technique2018

    • Author(s)
      Kazuki Tainaka, Rie Saito, Akiyoshi Kakita
    • Organizer
      ICN2018, 19th International Congress of Neuropathology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Book] 『病理と臨床』2020年3月号 組織・細胞の透明化2020

    • Author(s)
      田井中一貴
    • Total Pages
      4
    • Publisher
      文光堂
    • Related Report
      2019 Annual Research Report
  • [Book] 『ぶんせき』2019年10号 入門講座 組織透明化技術と蛍光顕微鏡 3D イメージング2019

    • Author(s)
      田井中一貴
    • Total Pages
      8
    • Publisher
      公益社団法人 日本分析化学会
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2018-07-25   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi