Development of 3D pathological analysis of cerebral small vessel disease
| Project/Area Number |
18K19373
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 46:Neuroscience and related fields
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2018-06-29 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 脳小血管病 / 組織透明化 / 3Dイメージング / 神経病理 |
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| Outline of Final Research Achievements |
In this study, we developed a novel tissue clearing protocol for postmortem human brain tissue, and whole-mount immunohistochemistry for visualizing vasculature-related molecules to achieve 3D imaging of cerebral small vessel disease. We obtained an efficient delipidation chemicals for human white matter, and succeeded to suppress strong autofluorescence derived from human brain such as lipofuscin. According to a series of these techniques, we analyzed denaturation of capillary, functional disorders of astrocyte, and white matter lesion in clinical tissue samples from such as CADASIL.
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| Academic Significance and Societal Importance of the Research Achievements |
脳小血管病は脳梗塞の再発、認知機能、歩行障害などと関連することが知られており、臨床の現場では病態の解明および予防策の検討が急務とされている。しかしながら、毛細血管を構成する細胞種の同定や周囲のグリア細胞との関係性、並びに血液脳関門の機能的破綻などを立体的かつ高解像度に可視化する研究手法が確立されていなかったため、治療戦略に繋がる病理所見を得ることが難しかった。本研究により、ヒト脳病理標本の高度な透明化技術と共に血管やその他の関連因子を立体的に可視化する汎用性の高い免疫染色プロトコールが得られた。従って、脳小血管病の3D病態解析を推進する上で必要なシーズとなる萌芽的技術が得られた。
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents.2018
Author(s)
Tainaka K, Murakami TC, Susaki EA, Shimizu C, Saito R, Takahashi K, Hayashi-Takagi A, Sekiya H, Arima Y, Nojima S, Ikemura M, Ushiku T, Shimizu Y, Murakami M, Tanaka KF, Iino M, Kasai H, Sasaoka T, Kobayashi K, Miyazono K, Morii E, Isa T, Fukayama M, Kakita A, Ueda HR.
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Journal Title
Cell Rep.
Volume: 24
Issue: 8
Pages: 2196-2210
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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