• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Generation of photoactivatable transcription molecules to control memory formation

Research Project

Project/Area Number 18K19377
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 46:Neuroscience and related fields
Research InstitutionKyoto University

Principal Investigator

Saneyoshi Takeo  京都大学, 医学研究科, 准教授 (00556201)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords遺伝子発現制御 / 記憶 / シナプス可塑性 / 光操作 / 転写因子 / CREB / CaMKIV / 光制御 / 学習 / 行動 / 光刺激 / 遺伝子発現
Outline of Final Research Achievements

To control memory formation and its recall, it is important to have tools to manipulate gene expression for memory. We succeeded to generate a photo-inducible module, which allows any protein can be transformed into light-dependent nuclear localized protein. So far, we generated photoactivatable CREB and CaMKIV using the photo-inducible module. Although these photoactivatable molecules behaved as light-dependent nuclear localization, they showed a basal transcriptional activity without light. Because it might be due to leaky localization of overexpressed proteins in nucleus, we needed to reduce expression level by screening promoters or drug-mediated gene expression systems such as the TET-on/off.
We have established a method by which an AAV viral vector-mediated transducing protein into brain tissue through intravenous injection. Using this method, we could manipulate transcription by light after screening the promoter system to control expression for the photoactivatable proteins.

Academic Significance and Societal Importance of the Research Achievements

本研究の光活性化型核内移行シグナルを利用すれば理論的にはどんなタンパク質でも光で核へ局在化できるため、構築にかかる試行錯誤を最小限にできる。この方法論により様々な核内シグナルを人為的に操作でき、新しい転写や染色体制御の発見につながると期待される。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2020 2019

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] The role of CaMKII-Tiam1 complex on learning and memory2019

    • Author(s)
      Kojima Hiroto、Rosendale Morgane、Sugiyama Yui、Hayashi Mariko、Horiguchi Yoko、Yoshihara Toru、Ikegaya Yuji、Saneyoshi Takeo、Hayashi Yasunori
    • Journal Title

      Neurobiology of Learning and Memory

      Volume: 166 Pages: 107070-107070

    • DOI

      10.1016/j.nlm.2019.107070

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] A regulatory mechanism of persistence of LTP and memory2020

    • Author(s)
      Takeo Saneyoshi
    • Organizer
      UK-Japan symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reciprocal activation within a kinase-effector complex underlying persistence of structural LTP2019

    • Author(s)
      Takeo Saneyoshi, Yasunori Hayashi
    • Organizer
      Neuro2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reciprocal activation within a kinase-effector complex underlying persistence of structural LTP2019

    • Author(s)
      Takeo Saneyoshi
    • Organizer
      Current Trends and Future Directions of Synapse-Circuit Plasticity Research
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シナプス機能の増強を維持する記憶のメカニズム2019

    • Author(s)
      實吉 岳郎
    • Organizer
      「脳構築の時計と場」「スクラップビルド」「マルチスケール脳」合同若手シンポジウム
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2018-07-25   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi