| Project/Area Number |
18K19443
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 49:Pathology, infection/immunology, and related fields
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| Research Institution | Osaka Prefecture University (2021)
Kyoto University (2018-2020) |
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Principal Investigator |
Horie Masayuki 大阪府立大学, 生命環境科学研究科, 教授 (20725981)
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| Project Period (FY) |
2018-06-29 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ボルナ病ウイルス / RNA編集 / Cre/loxP部位特異的組換え / ADAR1 / 進化 / ボルナウイルス / Cre / 感染排除 / 病原性 / Cre/loxP |
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| Outline of Final Research Achievements |
In this study, we utilized genetic recombination technology to create an experimental system that can permanently label cells once infected with Borna disease virus 1 (BoDV-1), which causes functional defects in host neurons. This experimental system has made it possible to evaluate the function of cells that have been infected with the virus and then eliminated them by drugs or other means. However, although this system worked well in cultured cell lines, it did not work well in infected neurons of animals.
On the other hand, we found many mutations in the Cre gene sequence inserted into recombinant BoDV-1 that were thought to be caused by the host's RNA editing enzyme, revealing a possible novel interaction between the virus and the host.
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| Academic Significance and Societal Importance of the Research Achievements |
近年、BoDV-1は人獣共通感染症としても再注目を浴びている。本研究によって作出した技術は、今後のBoDV-1の新たな病態・病原性の可能性を評価するうえで極めて重要な基盤となる。さらには想定外の成果として宿主RNA編集酵素との相互作用によるウイルスゲノムの変異が示唆されており、今後のウイルス-宿主細胞間相互作用のさらなる解明や、またウイルスの進化についてに有用な知見が得られた。
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