| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Extracellular vesicles are regulators of miRNA-mediated intercellular communications to maintain malignant properties of cancer cells. However, detailed mechanisms of how specific miRNAs selected and secreted from cancer cells are not fully understood. We aimed to understand the mechanism of miRNA secretion and to classify extracellular miRNAs by their functions. We found secretion of a similar subset of miRNAs from sarcoma cells. Two of them were found to induce the differentiation of monocyte to macrophage. Protein X was suggested to be secreted as vesicles (or complexes), which are distinct from exosomes. The RNA processing proteins and specific miRNAs were enriched in the protein X fraction. Secretion of these miRNAs were not affected by an exosome inhibitor. Screening intracellular molecules indicated the binding of the protein X with Exportins and RAB family proteins. These results suggest that the protein X is regulator for the intracellular protein and miRNA trafficking.
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