Novel ubiquitin-like protein encoded by long non-coding RNAs

• Project/Area Number: 18K19549
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 53:Organ-based internal medicine and related fields
• Research Institution: Kyushu University
• Principal Investigator: Akinobu Matsumoto 九州大学, 生体防御医学研究所, 准教授 (60741519)
• Project Period (FY): 2018-06-29 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: Long non-coding RNA / ユビキチン様タンパク質 / 皮膚 / ノンコーディングRNA / 皮膚疾患

Outline of Final Research Achievements

We have identified a novel protein that is translated from an RNA that has been reported as a long non-coding RNA. The protein is widely conserved from birds to mammals, is expressed in a skin-specific manner, and has a ubiquitin-like domain.
To elucidate the role of this novel ubiquitin-like protein in keratinocytes, we analyzed the human primary keratinocytes overexpressing this protein and epidermis derived from knockout mice, and found that this protein regulates the cell cycle. Furthermore, we found that skin regeneration was significantly delayed in mice deficient in this protein.

Academic Significance and Societal Importance of the Research Achievements

われわれが同定した新規ユビキチン様タンパク質は、これまでlong non-coding RNAと思われていたRNAから翻訳されていた。long non-coding RNAがタンパク質を翻訳しているということも含め、まだ未知のユビキチン様タンパク質が存在していたという発見は学術的にも意義が高い。またこの新規ユビキチン様タンパク質は皮膚の再生に重要であることが明らかとなり、臨床的な意義も高い知見である。

Research Products

• [Journal Article] Cell cycle-dependent localization of the proteasome to chromatin (2020)
  • Author(s): Kito Yuki、Matsumoto Masaki、Hatano Atsushi、Takami Tomoyo、Oshikawa Kiyotaka、Matsumoto Akinobu、Nakayama Keiichi I.
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 5801-5801
  • DOI: 10.1038/s41598-020-62697-2
  • Peer Reviewed / Open Access
• [Journal Article] Intragenic antagonistic roles of protein and circRNA in tumorigenesis (2019)
  • Author(s): Guarnerio J, Zhang Y, Cheloni G, Panella R, Mae Katon J, Simpson M, Matsumoto A, Papa A, Loretelli C, Petri A, Kauppinen S, Garbutt C, Nielsen GP, Deshpande V, Castillo-Martin M, Cordon-Cardo C, Dimitrios S, Clohessy JG, Batish M, Pandolfi PP.
  • Journal Title: Cell Research Volume: 29 Issue: 8 Pages: 628-640
  • DOI: 10.1038/s41422-019-0192-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Hidden Peptides Encoded by Putative Noncoding RNAs (2018)
  • Author(s): Matsumoto A, Nakayama KI.
  • Journal Title: Cell Structure and Function Volume: 43 Issue: 1 Pages: 75-83
  • DOI: 10.1247/csf.18005
  • NAID: 130006742309
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed / Open Access
• [Presentation] 新規ポリペプチドによる恒常性維持機構 (2019)
  • Author(s): 松本 有樹修
  • Organizer: 第71回日本細胞生物学会大会
• [Presentation] Long non-coding RNAの解析から明らかとなった新規ポリペプチドワールド (2019)
  • Author(s): 松本 有樹修
  • Organizer: 第37回日本骨代謝学会学術集会
  • Invited
• [Presentation] Polypeptides world revealed by analyses of long non-coding RNAs (2019)
  • Author(s): 松本 有樹修
  • Organizer: 第42回日本分子生物学会年会
  • Int'l Joint Research
• [Presentation] Long non-coding RNAから翻訳される機能性ポリペプチド群の同定 (2018)
  • Author(s): 松本 有樹修
  • Organizer: 日本筋学会第4回学術集会
  • Invited
• [Presentation] Functional polypeptides encoded by putative long non-coding RNAs (2018)
  • Author(s): 松本 有樹修
  • Organizer: 第41回日本分子生物学会年会