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Measuring shape and movement of hematopoietic stem cells

Research Project

Project/Area Number 18K19570
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 54:Internal medicine of the bio-information integration and related fields
Research InstitutionNational Center for Global Health and Medicine

Principal Investigator

Takubo Keiyo  国立研究開発法人国立国際医療研究センター, その他部局等, 生体恒常性プロジェクト長 (50502788)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords造血幹細胞 / 造血前駆細胞 / 幹細胞性 / イメージング / 画像解析
Outline of Final Research Achievements

We established a two-dimensional platform for measuring “shape” and “movement” of hematopoietic stem cells. We next isolated steady-state hematopoietic stem cells and differentiated progenitor cells using a flow cytometer based on surface markers. Then, we collected data on "shape" and "moving" using the platform over time. In addition, by adding a fluorophore-labeled antibody to the culture, we analyze whether there is a change in "shape" and "movement" before and after the timing of differentiation. The obtained data were quantitatively analyzed using an image analysis program, and the characteristics specific to hematopoietic stem cells were obtained.

Academic Significance and Societal Importance of the Research Achievements

本研究より、表面マーカーに依存した細胞同定、という血液学研究の技術的ボトルネックの解消につながる成果が得られた。既存の手法とは全く違う方法からの細胞同定手法の萌芽が得られたといえ、造血分野にとどまらない創造的な外挿性・発展性のある技術への足掛かりになることが期待される。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (9 results)

All 2019 Other

All Int'l Joint Research (2 results) Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 4 results) Remarks (1 results)

  • [Int'l Joint Research] University of Cambridge(英国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Karolinska Institutet(スウェーデン)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Discrimination of dormant and active hematopoietic stem cells by G0 markers reveals dormancy regulation by cytoplasmic calcium.2019

    • Author(s)
      5.Fukushima T, Tanaka Y, Hamey FK, Chang CH, Oki T, Asada S, Hayashi Y, Fujino T, Yonezawa T, Takeda R, Kawabata KC, Fukuyama T, Umemoto T, Takubo K, Takizawa H, Goyama S, Ishihama Y, Honda H, Gottgens B, Kitamura T.
    • Journal Title

      Cell Rep

      Volume: 29 Issue: 12 Pages: 4144-4158

    • DOI

      10.1016/j.celrep.2019.11.061

    • NAID

      120006892032

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Type I Interferon Delivery by iPSC-Derived Myeloid Cells Elicits Antitumor Immunity via XCR1+ Dendritic Cells.2019

    • Author(s)
      Tsuchiya N, Zhang R, Iwama T, Ueda N, Liu T, Tatsumi M, Sasaki Y, Shimoda R, Osako Y, Sawada Y, Kubo Y, Miyashita A, Fukushima S, Cheng Z, Nakaki R, Takubo K, Okada S, Kaneko S, Ihn H, Kaisho T, Nishimura Y, Senju S, Endo I, Nakatsura T, Uemura Y.
    • Journal Title

      Cell Rep.

      Volume: 29 Issue: 1 Pages: 162-175

    • DOI

      10.1016/j.celrep.2019.08.086

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fasting-Refeeding Impacts Immune Cell Dynamics and Mucosal Immune Responses2019

    • Author(s)
      Nagai M、Noguchi R、Takahashi D、Morikawa T、Koshida K、Komiyama S、Ishihara N、Yamada T、Kawamura YI.、Muroi K、Hattori K、Kobayashi N、Fujimura Y、Hirota M、Matsumoto R、Aoki R、Tamura-Nakano M、Sugiyama M、Katakai T、Sato S、Takubo K、Dohi T、Hase K
    • Journal Title

      Cell

      Volume: 178 Issue: 5 Pages: 1072-1087

    • DOI

      10.1016/j.cell.2019.07.047

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo.2019

    • Author(s)
      Kobayashi H, Morikawa T, Okinaga A, Hamano F, Hashidate-Yoshida T, Watanuki S, Hishikawa D, Shindou H, Arai F, Kabe Y, Suematsu M, Shimizu T, Takubo K.
    • Journal Title

      Cell Reports

      Volume: 28 Issue: 1 Pages: 145-158

    • DOI

      10.1016/j.celrep.2019.06.008

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ATP turnover and glucose dependency in hematopoietic stem/progenitor cells are increased by proliferation and differentiation2019

    • Author(s)
      Watanuki Shintaro、Kobayashi Hiroshi、Sorimachi Yuriko、Yamamoto Masamichi、Okamoto Shinichiro、Takubo Keiyo
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 印刷中 Issue: 1 Pages: 287-294

    • DOI

      10.1016/j.bbrc.2019.04.123

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential involvement of semaphorin 3A in maintaining intervertebral disc tissue homeostasis2019

    • Author(s)
      Mima Y, Suzuki S, Fujii T, Morikawa T, Tamaki S, Takubo K, Shimoda M, Miyamoto T, Watanabe K, Matsumoto M, Nakamura M, Fujita N.
    • Journal Title

      J Orthop Res

      Volume: 37 Issue: 4 Pages: 972-980

    • DOI

      10.1002/jor.24258

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Remarks] 国立国際医療研究センター研究所生体恒常性プロジェクト公式ウェブサイト

    • URL

      https://takubolab.com/

    • Related Report
      2019 Annual Research Report 2018 Research-status Report

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Published: 2018-07-25   Modified: 2021-02-19  

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