Regulation of myogenesis via de novo DNA methylation
| Project/Area Number |
18K19603
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ASAHARA Hiroshi 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (70294460)
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| Project Period (FY) |
2018-06-29 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 筋分化 / DNAメチル化 / DNMT3A / Rp58 / ES細胞 |
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| Outline of Final Research Achievements |
The muscle differentiation has been a model for developmental and regenerative research since the discovery of the world's first master factor, the transcription factor MyoD. Furthermore, we have reported that the repressive transcription factor Rp58 is a key component of the gene network for muscle differentiation and that DNA methylation is essential for muscle development and regeneration as epigenomic information. On the basis of these findings, the present study clarified the mechanism of cell fate determination involving DNA methylation of Rp58 in muscle differentiation.
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| Academic Significance and Societal Importance of the Research Achievements |
発生・発達における DNAメチル化は、エピゲノム情報として、細胞の運命を決定するが、その詳細なメカニズムはまだ不明な点がおおい。我々はその鍵を握る遺伝子として、転写抑制因子Rp58とDNAメチル化酵素の相互関係に注目し、筋分化・再生の分子メカニズムの一端を明らかにすることができた。この成果は、今後、筋疾患の病態解明や治療への応用をはじめ、広く、体の恒常性維持、組織再生、発がんなど多くの医学・生物学研究の基盤となることが期待される。
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Report
(4 results)
Research Products
(55 results)
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[Journal Article] Transcriptome analysis of sevoflurane exposure effects at the different brain regions.2020
Author(s)
Yamamoto H, Uchida Y, Chiba T, Kurimoto R, Matsushima T, Inotsume M, Ishikawa C, Li H, Shiga T, Muratani M, Uchida T, Asahara H
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Journal Title
PLos One
Volume: 15
Issue: 12
Pages: e0236771-e0236771
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Ablation of miR-146b in mice causes hematopoietic malignancy2018
Author(s)
Mitsumura Takahiro、Ito Yoshiaki、Chiba Tomoki、Matsushima Takahide、Kurimoto Ryota、Tanaka Yoko、Kato Tomomi、Uchida Keisuke、Ito Takashi、Yamamoto Kouhei、Eishi Yoshinobu、Kitagawa Masanobu、Miyazaki Yasunari、Inase Naohiko、Asahara Hiroshi
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Journal Title
Blood Advances
Volume: 2
Issue: 23
Pages: 3483-3491
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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