| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Glioblastoma is characterized by diffuse infiltration into the normal brain. Invasive glioma stem cells (GSCs) is an underlying cause of treatment failure. Here we show that neural stem cells (NSCs) derived from CRISRP/Cas9-edited human induced pluripotent stem cell (hiPSC) expressing a suicide gene had high tumor-trophic migratory capacity, leading to marked in vivo anti-tumor effects. Time-lapse imaging of organotypic brain slice cultures first visualized the directional migration of NSCs toward GSCs and the bystander killing effect. The gene insertion to house-keeping gene locus provided higher and stable transgene expression than other common insertion sites. Our results indicate the potential benefit of NSC-based gene therapy for invasive GSCs. Furthermore, the present research concept may become a platform to promote clinical studies using hiPSC.
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