| Project/Area Number |
18K19637
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Medium-sized Section 57:Oral science and related fields
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
AOKI Kazuhiro 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (40272603)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
泉福 英信 国立感染症研究所, 細菌第一部, 室長 (20250186)
|
|---|
| Project Period (FY) |
2018-06-29 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | 慢性炎症 / Streptococcus mutans / 口腔内常在菌 / 肝臓 / 腎臓 / 顎下腺 / 骨密度 / 骨形成 / 血管内投与 / 急性炎症 / 炎症性細胞浸潤 / ルシフェラーゼ遺伝子 / リンパ球 / 口腔内細菌 / S. mutans / 静脈内投与 / 生菌 / 尾静脈注射 / 肝臓と腎臓 / 経時的生菌数 / 発癌 / 血栓形成 / ＤＮＡメチル化 / 動物疾患モデル |
|---|
| Outline of Final Research Achievements |
Chronic inflammation is shown to be a trigger of malignant transformation. Bacteria in the oral cavity enter the blood due to various causes. Twelve-week-old male BALB/c mice were used to clarify whether the oral bacterium causes chronic inflammation. Time-course studies of histological changes in the liver after endovascular inoculation of Streptococcus mutans were performed.
As a result, acute inflammation was caused on the 1st day after the inoculation of the bacterium. However, the infiltration of lymphocytes, which is a chronic inflammation marker, gradually increased. On the 28th day after the endovascular inoculation, the rate of lymphocytes in the region the infiltrating cells showed a significant increase compared to that on day 1. These results suggest that exposure to Streptococcus mutans may cause chronic inflammation of tissues.
|
| Academic Significance and Societal Importance of the Research Achievements |
口腔内細菌叢の乱れが、全身健康を脅かすことが明らかになってきているが、この実験では、一つの菌と全身健康との関連を調べた。
血管を介した口腔内細菌の全身暴露が、癌化の一要因とされる慢性炎症を引き起こしたことは、常在菌であっても、いったん血管を介して体内に入れば、生体組織に生菌が定着し、炎症を引き起こし、慢性化させる可能性があることを示唆している。これらの研究結果は、歯科医療の重要性を社会に示す一つのエビデンスとなる。
|
