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Measurement of ER stress without destruction

Research Project

Project/Area Number 18K19640
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 57:Oral science and related fields
Research InstitutionOsaka University

Principal Investigator

Murakami Tomohiko  大阪大学, 歯学研究科, 講師 (50510723)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords小胞体ストレス / プロテオーム解析 / オルガネラ障害 / 小胞体
Outline of Final Research Achievements

To identify proteins released to extracellular space in response to endoplasmic reticulum (ER) stress, we searched for the proteins by proteome analysis. As a result, we found a protein that is released into the cell supernatant after ER stress. To investigate the function of the protein, we analyzed gene expression of cells stimulated with or without the recombinant protein. These results indicated that the protein strongly induced the expression of inflammation-related genes. Taken together, these findings suggested that the protein acts as an ER stress indicator and also functions as a novel inflammation inducer.

Academic Significance and Societal Importance of the Research Achievements

小胞体ストレス依存的に細胞外に放出される因子の同定により、非破壊的に小胞体ストレス状態を測定する技術の確立に近づいた。この技術の応用による小胞体ストレス関連疾患に対する新規予測診断法の確立は、内分泌、骨軟骨代謝疾患、神経を含む難病の予防あるいは早期治療への応用に発展していくことが期待される。さらに、今回同定した因子は新規の炎症誘導物質としても機能することから、小胞体ストレスインディケーターとしての役目だけではなく疾患自体との関連性も考えられる。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (7 results)

All 2020 2019 2018

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Role of Signal Transduction Pathways and Transcription Factors in Cartilage and Joint Diseases2020

    • Author(s)
      Nishimura Riko、Hata Kenji、Takahata Yoshifumi、Murakami Tomohiko、Nakamura Eriko、Ohkawa Maki、Ruengsinpinya Lerdluck
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 4 Pages: 1340-1340

    • DOI

      10.3390/ijms21041340

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cutting Edge: G Protein Subunit b 1 Negatively Regulates NLRP3 Inflammasome Activation2019

    • Author(s)
      Tomohiko Murakami, Lerdluck Ruengsinpinya, Eriko Nakamura,Yoshifumi Takahata, Kenji Hata, Hiroaki Okae,Shun’ichiro Taniguchi, Masafumi Takahashi and Riko Nishimura
    • Journal Title

      The Journal of Immunology

      Volume: 202 Issue: 7 Pages: 1942-1947

    • DOI

      10.4049/jimmunol.1801388

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Interaction of LEF1 with TAZ is necessary for the osteoblastogenic activity of Wnt3a2018

    • Author(s)
      Kida Jumpei、Hata Kenji、Nakamura Eriko、Yagi Hiroko、Takahata Yoshifumi、Murakami Tomohiko、Maeda Yoshinobu、Nishimura Riko
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 10375-10375

    • DOI

      10.1038/s41598-018-28711-4

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 小胞体ストレスセンサーOASISによる骨形成制御2018

    • Author(s)
      村上智彦
    • Journal Title

      THE BONE

      Volume: 32 Pages: 43-47

    • Related Report
      2018 Research-status Report
  • [Presentation] Gタンパク質サブユニットbeta 1はNLRP3インフラマソームを負に制御する2019

    • Author(s)
      村上智彦、Lerdluck Ruengsinpinya、中村恵理子、高畑佳史、波多賢二、西村理行
    • Organizer
      第6回JCRベーシックリサーチカンファレンス
    • Related Report
      2019 Annual Research Report
  • [Presentation] ER stress and bone metabolism2019

    • Author(s)
      Murakami T
    • Organizer
      日本内分泌学会学術総会 日韓シンポジウム
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 転写因子Sox4 はADAMTS4 およびADAMTS5 の発現を誘導して変形性関節症の発症に関与する2018

    • Author(s)
      高畑佳史、中村恵理子、波多賢二、若林真、村上智彦、若森幹太、吉川浩史、松田昭生、福井尚志、西村理行
    • Organizer
      日本骨代謝学会学術集会
    • Related Report
      2018 Research-status Report

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Published: 2018-07-25   Modified: 2021-02-19  

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