| Project/Area Number |
18K19640
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 57:Oral science and related fields
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2018-06-29 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 小胞体ストレス / プロテオーム解析 / オルガネラ障害 / 小胞体 |
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| Outline of Final Research Achievements |
To identify proteins released to extracellular space in response to endoplasmic reticulum (ER) stress, we searched for the proteins by proteome analysis. As a result, we found a protein that is released into the cell supernatant after ER stress. To investigate the function of the protein, we analyzed gene expression of cells stimulated with or without the recombinant protein. These results indicated that the protein strongly induced the expression of inflammation-related genes. Taken together, these findings suggested that the protein acts as an ER stress indicator and also functions as a novel inflammation inducer.
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| Academic Significance and Societal Importance of the Research Achievements |
小胞体ストレス依存的に細胞外に放出される因子の同定により、非破壊的に小胞体ストレス状態を測定する技術の確立に近づいた。この技術の応用による小胞体ストレス関連疾患に対する新規予測診断法の確立は、内分泌、骨軟骨代謝疾患、神経を含む難病の予防あるいは早期治療への応用に発展していくことが期待される。さらに、今回同定した因子は新規の炎症誘導物質としても機能することから、小胞体ストレスインディケーターとしての役目だけではなく疾患自体との関連性も考えられる。
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