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Development of novel cartilage regeneration therapy by glycotechnology

Research Project

Project/Area Number 18K19645
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 57:Oral science and related fields
Research InstitutionOsaka University

Principal Investigator

Inubushi Toshihiro  大阪大学, 歯学研究科, 講師 (30550941)

Co-Investigator(Kenkyū-buntansha) 佐々木 淳一  大阪大学, 歯学研究科, 講師 (50530490)
飛澤 悠葵  弘前大学, 医学研究科, 助教 (70623768)
Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords糖鎖 / 軟骨再生 / iPS / 軟骨分化 / ヘパラン硫酸 / 軟骨細胞 / 再生 / へパラン硫酸
Outline of Final Research Achievements

In the present study, we examined the optimal cell culture condition for iPS cells. We established the protocol for the appropriate culture and storage condition of iPS cells with maintaining pluripotency. It has a great contribution for the development of the cell culture and the experimental system. In addition, the modification of heparan sulfate proteoglycan remarkably enhances the chondrogenic differentiation of iPS cells. These results suggest the importance of cell surface glycan in iPS cell differentiation and the modification of the glycan could be the potent cartilage regeneration therapy. It was expected that further glycomic approach might help developing the novel cartilage regeneration therapy by the glycotechnology.

Academic Significance and Societal Importance of the Research Achievements

軟骨組織は神経・血管をもたないため、損傷されると自然修復が極めて困難な組織として知られており、現在、頭蓋・顎・顔面領域の先天性奇形や、外傷による組織の変形などに対する軟骨再生医療技術の開発が大いに期待されている。本研究は、iPS細胞表面の糖鎖編集を行うことで軟骨細胞分化を著しく促進できることを示したものである。このような取り組みはこれまでになく、学術的意義がある。本研究成果により、従来の方法では困難であった軟骨損傷に対する軟骨再生治療が可能となることが期待できる。このように、本研究成果は、軟骨再生医療開発に大きく寄与するものであり、大きな社会的意義がある。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2019

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (8 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Anterior cleft palate due to Cbfb deficiency and its rescue by folic acid2019

    • Author(s)
      Sarper Safiye E.、Inubushi Toshihiro、Kurosaka Hiroshi、Ono Minagi Hitomi、Murata Yuka、Kuremoto Koh-ichi、Sakai Takayoshi、Taniuchi Ichiro、Yamashiro Takashi
    • Journal Title

      Disease Models & Mechanisms

      Volume: 12 Issue: 6 Pages: 038851-038851

    • DOI

      10.1242/dmm.038851

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Observation of Dynamic Cellular Migration of the Medial Edge Epithelium of the Palatal Shelf in vitro2019

    • Author(s)
      Aoyama Gozo、Kurosaka Hiroshi、Oka Ayaka、Nakatsugawa Kohei、Yamamoto Sayuri、Sarper Safiye Esra、Usami Yu、Toyosawa Satoru、Inubushi Toshihiro、Isogai Yukako、Yamashiro Takashi
    • Journal Title

      Frontiers in Physiology

      Volume: 10

    • DOI

      10.3389/fphys.2019.00698

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel PTCH1 mutation in basal cell nevus syndrome with rare craniofacial features2019

    • Author(s)
      Murata Y, Kurosaka H, Ohata Y, Aikawa T, Takahata S, Fujii K, Miyashita T, Morita C, Inubushi T, Kubota T, Sakai N, Ozono K, Kogo M, Yamashiro T.
    • Journal Title

      Human Genome Variation

      Volume: 6 Issue: 1 Pages: 16-16

    • DOI

      10.1038/s41439-019-0047-9

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] A case report of Ehlers-Danlos syndrome patient with craniofacial anomaly and severe enamel hypoplasia2019

    • Author(s)
      Morita C, Inubushi T, Tanikawa C, Yamashiro T
    • Organizer
      Scientific meeting on the rarer types of Ehlers-Danlos syndromes
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The role of Wnt/β-catenin signaling in osteocyte differentiation2019

    • Author(s)
      Kusano S, Inubushi T, Murata Y, Sasaki J, Itoh S, Imazato S, Yamashiro T
    • Organizer
      The 4th meeting of the international Association for Dental Research Asia Pacific Region
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Osteocyte specific hyaluronic acid disruption reduced bone density in mice model2019

    • Author(s)
      Morita C, Inubushi T, Yamaguchi Y, Yamashiro T
    • Organizer
      The 4th meeting of the international Association for Dental Research Asia Pacific Region
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elucidation of the regulatory mechanisms of heparan sulfate on the craniofacial and tooth morphogenesis2019

    • Author(s)
      Nakanishi Y, Inubushi T, Matsumoto Y, Yamaguchi Y, Yamashiro T
    • Organizer
      The 4th meeting of the international Association for Dental Research Asia Pacific Region
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新規遺伝子抑制法を用いた口蓋形成の制御機構解明への新たな取り組み2019

    • Author(s)
      犬伏俊博、廣瀬 匠、吉田尚起、黒坂 寛、杉山 弘、山城 隆
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] コンドロイチン硫酸合成酵素 1 ノックアウトマウスを用いた変形性関節症の発症機構解明の試み2019

    • Author(s)
      犬伏俊博、松本 和、入江敏文、山城 隆、山口 祐
    • Organizer
      第37回日本骨代謝学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 骨細胞特異的ヒアルロン酸合成酵素ノックアウトマウスの解析2019

    • Author(s)
      森田知里、犬伏俊博、伊藤慎将、宇佐美 悠、豊澤 悟、山口 祐、山城 隆
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 骨細胞分化や分化段階の維持におけるWnt-βカテニン経路の役割2019

    • Author(s)
      草野慎之介、犬伏俊博、村田有香、伊藤慎将、黒坂 寛、佐々木淳一、山城 隆
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report

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Published: 2018-07-25   Modified: 2021-02-19  

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