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Elucidation of the mechanism of the regulation of Col1a1 gene expression by Runx2

Research Project

Project/Area Number 18K19654
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 57:Oral science and related fields
Research InstitutionNagasaki University

Principal Investigator

KOMORI Toshihisa  長崎大学, 医歯薬学総合研究科(歯学系), 教授 (00252677)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
KeywordsI型コラーゲン / Runx2 / 骨粗鬆症 / 骨芽細胞 / I型コラーゲン / 転写
Outline of Final Research Achievements

Type I collagen is a major organic substrate and composes more than 90% of bone. Type I collagen is composed of two α1 strands and one α2 strand forming a triple helical structure. Theα1 strand is encoded by Col1a1, and the α2 strand is encoded by Col1a2. 2.3 kb DNA upstream of the transcription start site has been shown to regulate Col1a1 expression in osteoblasts. However, it still remains to be clarified how Col1a1 expression is regulated. We found that Runx2, which is a master transcription factor for osteoblast differentiation, plays an important role in the regulation of Col1a1 expression in osteoblasts.

Academic Significance and Societal Importance of the Research Achievements

骨粗鬆症は国内だけでも1280万人おり、高齢化に伴いその数は年々増加している。しかし、長期間使える骨形成促進剤は存在しない。I型コラーゲンは、骨の大部分を占める重要な基質タンパク質であり、その発現制御機構の解明は、骨粗鬆症の治療薬の開発や、骨再生療法の開発に非常に重要である。したがって、今回その発現制御機構の一端を明らかにしたことは、今後その全容解明につながり学術的に重要であるのはもちろんであるが、骨形成促進剤の開発にも大きく貢献する。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (26 results)

All 2020 2019 2018

All Journal Article (14 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 14 results,  Open Access: 6 results) Presentation (12 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Osteocalcin is necessary for the alignment of apatite crystallites, but not glucose metabolism, testosterone synthesis, or muscle mass.2020

    • Author(s)
      Moriichi T, Ozasa R, Ishimoto T, Nakano T, Hasegawa T, Wenguang L, Fukuyama R, Yuying W, Komori H, Qin X, Amizuka N, Komori T
    • Journal Title

      PLOS Genet

      Volume: -

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of a constitutively active form of Hack in chondrocytes Int and hedgehog signaling pathways, and induces chondrocyte proliferation in mice.2020

    • Author(s)
      Kawata-Matsura V, Yoshida CA, Komori H, Sakane C, Yamana K, Jiang Q, Komori T
    • Journal Title

      Int J Mol Sci.

      Volume: 21 Pages: 2682-2682

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antxrl, which is a target of Runx2, regulates chondrocyte proliferation and apoptosis.2020

    • Author(s)
      Jiang Q, Qin X, Yoshida CA, Komori H, Yamana K, Oba S, Hojo H, Croix BS, Kawata-Matsuura V, Komori T
    • Journal Title

      Int J Mol Sci

      Volume: 21 Pages: 2425-2425

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of Proliferation, Differentiation and Functions of Osteoblasts by Runx22019

    • Author(s)
      Komori Toshihisa
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 20 Issue: 7 Pages: 1694-1694

    • DOI

      10.3390/ijms20071694

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Parathyroid Hormone Shifts Cell Fate of a Leptin Receptor-Marked Stromal Population from Adipogenic to Osteoblastic Lineage.2019

    • Author(s)
      Yang M, Arai A, Udagawa N, Zhao L, Nishida D, Murakami K, Hiraga T, Takao-Kawabata R, Matsuo K, Komori T, Kobayashi Y, Takahashi N, Isogai Y, Ishizuya T, Yamaguchi A, Mizoguchi T
    • Journal Title

      J Bone Miner Res

      Volume: 34(10) Pages: 1952-1963

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Runx2 regulates cranial suture closure by inducing hedgehog, Fgf, Int, and Pthlh signaling pathway gene expression in suture mesenchymal cells.2019

    • Author(s)
      Qin X, Jiang Q, Miyazaki T, Komori T
    • Journal Title

      Hum Mol Genet

      Volume: 28(6) Pages: 896-911

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Constitutive activation of the alternative NF-K B pathway disturbs endochondral ossification.2019

    • Author(s)
      Nakatomi C, Nakatomi M, Matsubara T, Doi-Inoue T, Ishimaru N, Weih F, Iwamoto T, Masuda M,Kokabu S, Jimi E
    • Journal Title

      Bone

      Volume: 121 Pages: 29-41

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sphenoid bone hypoplasia is a skeletal phenotype of cleidocranial dysplasia in a mouse model and patients.2019

    • Author(s)
      Mitoma K, Matsunaga S, Kitamura T, Saito A, Komori T, Muramatsu T, Yamaguchi A
    • Journal Title

      Bone

      Volume: 120 Pages: 176-186

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Runx2 regulates cranial suture closure by inducing hedgehog, Fgf, Wnt and Pthlh signaling pathway gene expressions in suture mesenchymal cells2019

    • Author(s)
      Qin Xin、Jiang Qing、Miyazaki Toshihiro、Komori Toshihisa
    • Journal Title

      Human Molecular Genetics

      Volume: 28 Issue: 6 Pages: 896-911

    • DOI

      10.1093/hmg/ddy386

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sphenoid bone hypoplasia is a skeletal phenotype of cleidocranial dysplasia in a mouse model and patients2019

    • Author(s)
      Mitomo Keisuke、Matsunaga Satoru、Kitamura Kei、Nakamura Takashi、Saito Akiko、Komori Toshihisa、Muramatsu Takashi、Yamaguchi Akira
    • Journal Title

      Bone

      Volume: 120 Pages: 176-186

    • DOI

      10.1016/j.bone.2018.10.028

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Collapsin Response Mediator Protein 1, a Novel Marker Protein for Differentiated Odontoblasts2018

    • Author(s)
      Miyazaki Toshihiro、T. Baba Tomomi、Mori Masako、Komori Toshihisa
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 51 Issue: 6 Pages: 185-190

    • DOI

      10.1267/ahc.18030

    • NAID

      130007535935

    • ISSN
      0044-5991, 1347-5800
    • Year and Date
      2018-12-20
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Loss of Stemness, EMT, and Supernumerary Tooth Formation in Cebpb-/-Runx2+/- Murine Incisors.2018

    • Author(s)
      Saito K, Takahashi K, Huang B, Asahara M, Kiso H, Togo Y, Tsukamoto H, Mishima S, Nagata M, Iida M, Tokita Y, Asai M, Shimizu A, Komori T, Harada H, MacDougall M, Sugai M, Bessho K.
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 5169-5169

    • DOI

      10.1038/s41598-018-23515-y

    • NAID

      120006545187

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Runx2 is required for the proliferation of osteoblast progenitors and induces proliferation by regulating Fgfr2 and Fgfr32018

    • Author(s)
      Kawane Tetsuya、Qin Xin、Jiang Qing、Miyazaki Toshihiro、Komori Hisato、Yoshida Carolina Andrea、Matsuura-Kawata Viviane Keiko dos Santos、Sakane Chiharu、Matsuo Yuki、Nagai Kazuhiro、Maeno Takafumi、Date Yuki、Nishimura Riko、Komori Toshihisa
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 13551-13551

    • DOI

      10.1038/s41598-018-31853-0

    • NAID

      120006987946

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Runx2, an inducer of osteoblast and chondrocyte differentiation2018

    • Author(s)
      Komori T
    • Journal Title

      Histochem Cell Biol

      Volume: 印刷中 Issue: 4 Pages: 313-323

    • DOI

      10.1007/s00418-018-1640-6

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] What is a real function of osteocalcin?2019

    • Author(s)
      Komori T
    • Organizer
      2019 International Symposium, Tokyo Dental college Research Branding
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Osteocalcin is required for the alignment of biological apatite crystallites but not for the regulation of bone quantity, glucose metabolism, testosterone synthesis, and muscle mass.2019

    • Author(s)
      Komori T
    • Organizer
      The 22nd International RUNX Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Osteolcin is required for the alignment of biological apatite crystallites but not for the regulation of bone formation, glucose metabolism testosterone synthesis, and muscle mass.2019

    • Author(s)
      Komori T, Ozasa R, Ishimoto T, nakano T, Moriishi T
    • Organizer
      ECTS 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 特別講演「Runx2による骨格形成制御機構」2019

    • Author(s)
      小守壽文
    • Organizer
      第124回日本解剖学会全国学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Runx2 と歩んだ骨代謝研究。2019

    • Author(s)
      小守壽文
    • Organizer
      第37回日本骨代謝学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 象牙質形成におけるゴルジ体キナーゼFam20Cの役割。2019

    • Author(s)
      廣瀬勝俊、宇佐美悠、大家香織、小守壽文、豊澤悟
    • Organizer
      第37回日本骨代謝学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Runx2による骨格形成制御機構2019

    • Author(s)
      小守 壽文
    • Organizer
      第124回日本日本解剖学会全国学術集会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Runx2による骨芽細胞分化・増殖の制御機構2018

    • Author(s)
      小守 壽文
    • Organizer
      第36回日本骨代謝学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] オステオカルシンはアパタイト結晶のコラーゲン線維に沿った配向に必須であり、長軸方向の骨強度を維持する2018

    • Author(s)
      森石武史,小笹良輔,中野貴由,宮崎敏博,小守壽文
    • Organizer
      第36回日本骨代謝学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Runx2は骨芽細胞分化後の骨基質蛋白質遺伝子発現に必要である2018

    • Author(s)
      姜晴,秦昕,宮崎敏博,福山亮,小守寿人,森石武史,伊藤公成,小守壽文
    • Organizer
      第36回日本骨代謝学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Runx2による頭蓋冠形成制御機構2018

    • Author(s)
      秦昕,姜晴,宮崎敏博,森石武史,小守寿人,小守壽文
    • Organizer
      第36回日本骨代謝学会学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 骨形成におけるゴルジ体キナーゼFam20Cの役割2018

    • Author(s)
      廣瀬勝俊,宇佐美悠,佐藤淳,大家香織,小守壽文,豊澤悟
    • Organizer
      第36回日本骨代謝学会学術集会
    • Related Report
      2018 Research-status Report

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Published: 2018-07-25   Modified: 2021-12-27  

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