| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We aimed to develop a method for selectively concentrating a virus from sewage using an antibody. The method was developed by using bacteriophage MS2. The cultured MS2 was immunoprecipitated by adding an anti-MS2 antibody. The amount of MS2 recovered was quantified by reverse transcription (RT)-qPCR. As a result, the amount of MS2 recovered increased depending on the amount of antibody input. Anti-MS2 antibody did not precipitate bacteriophage Qβ. Furthermore, it was confirmed by the culture method that culturable MS2 could be recovered by immunoprecipitation. Then we confirmed by reverse RT-qPCR that norovirus in sewage could also be concentrated by immunoprecipitation. From these results, it was demonstrated that the infectious virus could be selectively concentrated by immunoprecipitation.
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