Development and application of high-throughput sensitive quantitative assays for neutral lipids to prevent atherosclerosis
| Project/Area Number |
18K19736
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
池田 義人 滋賀医科大学, 医学部, 客員助教 (40736980)
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| Project Period (FY) |
2018-06-29 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 中性脂質 / 脂質 |
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| Outline of Final Research Achievements |
Acylglycerols (monoacylglycerol, diacylglycerol and triacylglycerol) and cholesterol play important roles in biological activities. The plasma levels of triacylglycerol and cholesterol are diagnostic criteria for dyslipidemia and metabolic syndrome. However, there is no high-throughput assay for quantifying acylglycerols or cholesterol with high sensitivity and specificity. In this study, we developed the specific enzymatic fluorometric assays to specifically measure acylglycerols and cholesterol.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究課題で開発した酵素蛍光定量法は、従来の方法と比べて非常に高感度で、主な操作はピペットによる試料と反応液のマイクロプレートへの分注であり、定量に要する時間は短時間で、ハイスループット定量が可能である。本酵素蛍光定量法は、動脈硬化性疾患についての研究だけでなく、動物実験や植物・微生物実験を含む生化学研究分野や栄養科学分野、薬理・創薬科学分野など生命科学全般に応用されることが期待できる。
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Report
(5 results)
Research Products
(33 results)
