| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The purpose of this study is to develop an evaluation system for tissue aging by measuring cytoskeleton disruption-induced DNA damage after heat treatment. Heat treatment induced histone H2AX phosphorylation (γ-H2AX), known as a DNA damage marker, more remarkably in spleen lymphocytes from aged mice than that of young mice. The similar trend was observed when human normal skin fibroblasts were used. The cells of high passages (aged cells) showed strong γ-H2AX generation compared with that of low passages. Filament actin in aged cells was vulnerable to heat stress. These results suggested that detection of γ-H2AX after heat treatment may be a good index to evaluate tissue aging.
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