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DNA public cryptography

Research Project

Project/Area Number 18K19777
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 60:Information science, computer engineering, and related fields
Research InstitutionThe University of Tokyo

Principal Investigator

Yachie Nozomu  東京大学, 先端科学技術研究センター, 准教授 (60636801)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords公開鍵暗号 / DNA / DNAバーコード
Outline of Final Research Achievements

The security of modern public-key cryptography, such as RSA cryptography, is based on the fact that it is difficult to factorize giant numbers into prime numbers. In addition, the security of public-key cryptography systems has been guaranteed by a high degree of unidirectionality in the conversion from a public key generation to a private key, and in the data encryption using a public key. However, it has recently been shown that quantum computers are, in theory, capable of fast prime factorization (Shor's Theorem). Therefore, we need a new public-key cryptosystem for the non-von Neumann computer era. In this study, we proposed a DNA-based public cryptography system Barcode Split Genetics (BSG). BSG is based on the irreversible truncation of spatially constrained, highly complex DNA molecules. Our experimental results showed the high potential of this new cryptography system.

Academic Significance and Societal Importance of the Research Achievements

通信の秘匿性は国家戦略、企業経営から個人のプライバシー保護まで、社会のあらゆる場面で重要であり、今日世界中の情報通信インフラは公開鍵暗号システムに支えられている。一方で、量子コンピュータ等、非ノイマン型コンピュータの今後5年以内の実現がSFではなくなり、その様な計算資源によって現代の暗号システムは無効化される可能性が高い。量子コンピュータや光コンピュータを利用した新たな暗号システムも理論上は展開されているが、非ノイマン型コンピュータの登場以前に実現の見込みがあるものはなく、新たな強い暗号システムの開発は喫緊の課題である。この問題を解決しうるDNAを基礎にしたBSG暗号システムを開発した。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (31 results)

All 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (16 results) (of which Int'l Joint Research: 12 results,  Peer Reviewed: 15 results,  Open Access: 6 results) Presentation (10 results) (of which Int'l Joint Research: 6 results,  Invited: 9 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Int'l Joint Research] Laval University/University of Toronto/Mt Sinai Hospital(カナダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Toronto/Laval University(カナダ)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Highly Combinatorial Genetic Interaction Analysis Reveals a Multi-Drug Transporter Influence Network2020

    • Author(s)
      Celaj Albi、Gebbia Marinella、Musa Louai、Cote Atina G.、Snider Jamie、Wong Victoria、Ko Minjeong、Fong Tiffany、Bansal Paul、Mellor Joseph C.、Seesankar Gireesh、Nguyen Maria、Zhou Shijie、Wang Liangxi、Kishore Nishka、Stagljar Igor、Suzuki Yo、Yachie Nozomu、Roth Frederick P.
    • Journal Title

      Cell Systems

      Volume: 10 Issue: 1 Pages: 25-38.e10

    • DOI

      10.1016/j.cels.2019.09.009

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice2020

    • Author(s)
      Nishizono Hirofumi、Darwish Mohamed、Uosaki Hideki、Masuyama Nanami、Seki Motoaki、Abe Hiroyuki、Yachie Nozomu、Yasuda Ryohei
    • Journal Title

      Journal of Visualized Experiments

      Volume: - Issue: 158 Pages: 1-1

    • DOI

      10.3791/60808

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Perturbing proteomes at single residue resolution using base editing2020

    • Author(s)
      Despres Philippe C.、Dube Alexandre K.、Seki Motoaki、Yachie Nozomu、Landry Christian R.
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 1871-1871

    • DOI

      10.1038/s41467-020-15796-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Yeast-Based Genetic Interaction Analysis of Human Kinome2020

    • Author(s)
      Kim Jae-Hong、Seo Yeojin、Jo Myungjin、Jeon Hyejin、Lee Won-Ha、Yachie Nozomu、Zhong Quan、Vidal Marc、Roth Frederick P.、Suk Kyoungho
    • Journal Title

      Cells

      Volume: 9 Issue: 5 Pages: 1156-1156

    • DOI

      10.3390/cells9051156

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Base editors for simultaneous introduction of C-to-T and A-to-G mutations2020

    • Author(s)
      Sakata Rina C.、Ishiguro Soh、Mori Hideto、Tanaka Mamoru、Tatsuno Kenji、Ueda Hiroki、Yamamoto Shogo、Seki Motoaki、Masuyama Nanami、Nishida Keiji、Nishimasu Hiroshi、Arakawa Kazuharu、Kondo Akihiko、Nureki Osamu、Tomita Masaru、Aburatani Hiroyuki、Yachie Nozomu
    • Journal Title

      Nature Biotechnology

      Volume: na Issue: 7 Pages: 865-869

    • DOI

      10.1038/s41587-020-0509-0

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Complete Genome Sequence of Bacillus sp. Strain KH172YL63, Isolated from Deep-Sea Sediment2020

    • Author(s)
      Murai Yumi、Masuda Takahiro、Onuma Yasuhide、Evans-Yamamoto Daniel、Takeuchi Nao、Mori Hideto、Masuyama Nanami、Ishiguro Soh、Yachie Nozomu、Arakawa Kazuharu
    • Journal Title

      Microbiology Resource Announcements

      Volume: 9 Issue: 16

    • DOI

      10.1128/mra.00291-20

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA event recorders send past information of cells to the time of observation2019

    • Author(s)
      Ishiguro Soh、Mori Hideto、Yachie Nozomu
    • Journal Title

      Current Opinion in Chemical Biology

      Volume: 52 Pages: 54-62

    • DOI

      10.1016/j.cbpa.2019.05.009

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA barcodes evolve for high-resolution cell lineage tracing2019

    • Author(s)
      Masuyama Nanami、Mori Hideto、Yachie Nozomu
    • Journal Title

      Current Opinion in Chemical Biology

      Volume: 52 Pages: 63-71

    • DOI

      10.1016/j.cbpa.2019.05.014

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role of structural pleiotropy and regulatory evolution in the retention of heteromers of paralogs2019

    • Author(s)
      Marchant Axelle、Cisneros Angel F、Dube Alexandre K、Gagnon-Arsenault Isabelle、Ascencio Diana、Jain Honey、Aube Simon、Eberlein Chris、Evans-Yamamoto Daniel、Yachie Nozomu、Landry Christian R
    • Journal Title

      eLife

      Volume: 8

    • DOI

      10.7554/elife.46754

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A genome-scale CRISPR/Cas9 knockout screening reveals SH3D21 as a sensitizer for gemcitabine2019

    • Author(s)
      Masoudi Mohammad、Seki Motoaki、Yazdanparast Razieh、Yachie Nozomu、Aburatani Hiroyuki
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 19188-19188

    • DOI

      10.1038/s41598-019-55893-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The role of structural pleiotropy and regulatory evolution in the retention of heteromers of paralogs2019

    • Author(s)
      Marchant Axelle、Cisneros Angel F.、Dube Alexandre K、Gagnon-Arsenault Isabelle、Ascencio Diana、Jain Honey A.、Aube Simon、Eberlein Chris、Evans-Yamamoto Daniel、Yachie Nozomu、Landry Christian R
    • Journal Title

      bioRxiv

      Volume: NA

    • DOI

      10.1101/564401

    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Journal Article] Complete Genome Sequence of Psychrobacter sp. Strain KH172YL61, Isolated from Deep-Sea Sediments in the Nankai Trough, Japan2019

    • Author(s)
      Evans-Yamamoto Daniel、Takeuchi Nao、Masuda Takahiro、Murai Yumi、Onuma Yasuhide、Mori Hideto、Masuyama Nanami、Ishiguro Soh、Yachie Nozomu、Arakawa Kazuharu
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 16

    • DOI

      10.1128/mra.00326-19

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] beditor: A Computational Workflow for Designing Libraries of Guide RNAs for CRISPR-Mediated Base Editing2019

    • Author(s)
      Dandage Rohan、Despres Philippe C.、Yachie Nozomu、Landry Christian R.
    • Journal Title

      Genetics

      Volume: NA Issue: 2 Pages: 377-385

    • DOI

      10.1534/genetics.119.302089

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Fast and global detection of periodic sequence repeats in large genomic resources2018

    • Author(s)
      Mori Hideto、Evans-Yamamoto Daniel、Ishiguro Soh、Tomita Masaru、Yachie Nozomu
    • Journal Title

      Nucleic Acids Research

      Volume: 47 Issue: 2 Pages: e8-e8

    • DOI

      10.1093/nar/gky890

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Engineered CRISPR-Cas9 nuclease with expanded targeting space2018

    • Author(s)
      Nishimasu Hiroshi、Shi Xi、Ishiguro Soh、Gao Linyi、Hirano Seiichi、Okazaki Sae、Noda Taichi、Abudayyeh Omar O.、Gootenberg Jonathan S.、Mori Hideto、Oura Seiya、Holmes Benjamin、Tanaka Mamoru、Seki Motoaki、Hirano Hisato、Aburatani Hiroyuki、Ishitani Ryuichiro、Ikawa Masahito、Yachie Nozomu、Zhang Feng、Nureki Osamu
    • Journal Title

      Science

      Volume: 361 Issue: 6408 Pages: 1259-1262

    • DOI

      10.1126/science.aas9129

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast2018

    • Author(s)
      Despres Philippe C、Dube Alexandre K、Nielly-Thibault Lou、Yachie Nozomu、Landry Christian R
    • Journal Title

      G3: Genes|Genomes|Genetics

      Volume: 8 Issue: 10 Pages: 3163-3171

    • DOI

      10.1534/g3.118.200461

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] DNA barcode technologies to dissect heterogeneous biological systems2019

    • Author(s)
      Yachie N
    • Organizer
      The 13th Intn’l Workshop on Advanced Genomics
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Recording cellular events in DNA2019

    • Author(s)
      Yachie N
    • Organizer
      Asian Synthetic Biology Association
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Recording cellular events in DNA2019

    • Author(s)
      Yachie N
    • Organizer
      Frontiers in Genome Engineering
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Synthetic DNA memory devices to measure molecular and cellular dynamics2018

    • Author(s)
      谷内江 望
    • Organizer
      生命科学系フロンティアミーティング
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Developing new genome editing technologies2018

    • Author(s)
      谷内江 望
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] A new genetic marker to analyze molecular dynamics of a same clone in complex cell populations2018

    • Author(s)
      Nozomu Yachie
    • Organizer
      The 91st Annual Meeting of the Japanese Biochemical Society
    • Related Report
      2018 Research-status Report
  • [Presentation] Scaling up massively parallel experiments using DNA barcodes2018

    • Author(s)
      Nozomu Yachie
    • Organizer
      JST ImPACT Artificial Cell Program, Tokyo
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Chasing molecular and cellular dynamics using DNA barcodes2018

    • Author(s)
      Nozomu Yachie
    • Organizer
      Cell Mapping Symposium, San Diego, US
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] We do not have a time machine, but can install a recording system to a cell2018

    • Author(s)
      Nozomu Yachie
    • Organizer
      Asian Synthetic Biology Association 2018, Ceju, Korea
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Bringing the sense of amazing yeast genetics to mammalian biology2018

    • Author(s)
      谷内江 望
    • Organizer
      第22回酵母合同シンポジウム
    • Related Report
      2018 Research-status Report
    • Invited
  • [Remarks] Yachie Laboratory at the University of Tokyo

    • URL

      http://yachie-lab.org/

    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] 2種の核酸塩基変換酵素が融合されたCasタンパク質を利用したゲノム編集システム2019

    • Inventor(s)
      谷内江望、石黒宗、坂田莉奈、森秀人
    • Industrial Property Rights Holder
      谷内江望、石黒宗、坂田莉奈、森秀人
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 汎用的繰り返しDNAアセンブリ技術(FRACTALアセンブリ)2019

    • Inventor(s)
      谷内江望、山口七 海、森秀人
    • Industrial Property Rights Holder
      谷内江望、山口七 海、森秀人
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-053077
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

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Published: 2018-07-25   Modified: 2021-02-19  

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