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Development of Artificial Membrane System by DNA Membrane Motif

Research Project

Project/Area Number 18K19830
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 62:Applied informatics and related fields
Research InstitutionTohoku University

Principal Investigator

Murata Satoshi  東北大学, 工学研究科, 教授 (10334533)

Co-Investigator(Kenkyū-buntansha) 川又 生吹  東北大学, 工学研究科, 助教 (30733977)
Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
KeywordsDNA分子膜 / 人工細胞 / 分子ロボティクス / DNA膜 / DNAモチーフ / 人工膜
Outline of Final Research Achievements

Aiming at constructing artificial membrane with synthesized DNA , we designed a DNA membrane motif that dynamically binds via a kissing loop similar to the flow mosaic model. D-shaped and O-shaped motifs with a hard double-stranded stem and a soft single-stranded loop were prepared to evaluate the loop-loop interaction between membrane motifs. Although variations of motifs and various buffer condition were tested, the expected aggregation was not observed. We investigated a method to increase the formation rate of the structure by confining the motif in the closed space. As the specimen, we adopted two types of membranous DNA origami structures, which are known to be quickly formed in various conditions. As the result it was confirmed that the micro space is advantageous for the reliable formation of the structure.

Academic Significance and Societal Importance of the Research Achievements

本研究では,自在に機能を設計できる人工細胞の構築に向けて,非脂質分子による膜構造の形成を試みた.分子間の動的な組み換えを意図してキッシングループ間相互作用で凝集するDNAモチーフを設計したが,熱力学的な安定性(モチーフ間の結合強度)が設計値よりかなり小さく,環状構造のトポロジカルな拘束が強く働いていることが示唆された.そこで,対象をDNAオリガミに変更し,閉鎖空間に閉じ込めることにより膜状構造体の形成率を高める方法を検討した.これにより人工膜形成を促進する環境制御の指針が得られた.膜面の機能化が容易なDNAを素材とする人工膜が実現すれば,医療応用などさまざまな用途が開けることが期待される.

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (13 results)

All 2020 2019 2018

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 6 results) Presentation (5 results) Book (1 results)

  • [Journal Article] Programming methods for DNA based reaction-diffusion systems2020

    • Author(s)
      Keita Abe, Satoshi Murata
    • Journal Title

      New Generation Computing

      Volume: Online Pages: 1-15

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Large deformation of a DNA origami nanoarm through the cumulative actuation of tension-adjustable modules2020

    • Author(s)
      Yuki Suzuki, Ibuki Kawamata, Kohei Mizunom Satoshi Murata
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 59 Issue: 15 Pages: 6230-6234

    • DOI

      10.1002/anie.201916233

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DNA Origami “Quick” Refolding inside of a Micron-Sized Compartment2020

    • Author(s)
      Taiki Watanabe, Yusuke Sato, Hayato Otaka, Ibuki Kawamata, Satoshi Murata, Shin-Ichiro M. Nomura
    • Journal Title

      Molecules

      Volume: 25 Issue: 1 Pages: 8-8

    • DOI

      10.3390/molecules25010008

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pattern Formation on Discrete Gel Matrix based on DNA Computing2019

    • Author(s)
      Takuto Hosoya, Ibuki Kawamata, Shin-ichiro M. Nomura, Satoshi Murata
    • Journal Title

      New Generation Computing

      Volume: 37 Issue: 1 Pages: 97-111

    • DOI

      10.1007/s00354-018-0047-1

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] W-13 Developing Students Abilities by Design Competition2019

    • Author(s)
      S Murata, I Kawamata, Y Suzuki
    • Journal Title

      JSEE Annual Conference International Session Proceedings

      Volume: 2019 Issue: 0 Pages: 68-73

    • DOI

      10.20549/jseeen.2019.0_68

    • NAID

      130007741656

    • ISSN
      2189-8936, 2424-1466
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles2019

    • Author(s)
      Yusuke Sato, Ken Komiya, Ibuki Kawamata, Satoshi Murata, Shin-ichiro M. Nomura
    • Journal Title

      Chemical Communications

      Volume: 印刷中 Issue: 62 Pages: 9084-9087

    • DOI

      10.1039/c9cc03277k

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Programmable reactions and diffusion using DNA for pattern formation in hydrogel medium2019

    • Author(s)
      Keita Abe, Ibuki Kawamata, Shin-ichiro M. Nomura, Satoshi Murata
    • Journal Title

      Molecular Systems Design & Engineering

      Volume: accepted Issue: 3 Pages: 639-643

    • DOI

      10.1039/c9me00004f

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 相互作用力が調整可能なDNAモチーフの幾何学設計2019

    • Author(s)
      内田健央,川又生吹,野村慎一郎,村田智
    • Organizer
      第2回分子ロボティクス年次大会(東京工業大学)
    • Related Report
      2018 Research-status Report
  • [Presentation] キッシングループ相互作用によるDNAモチーフの構造化2019

    • Author(s)
      遠藤佑真,川又生吹,野村慎一郎,村田智
    • Organizer
      第2回分子ロボティクス年次大会(東京工業大学)
    • Related Report
      2018 Research-status Report
  • [Presentation] 脂質膜上に形成した2次元DNAナノ構造の評価2018

    • Author(s)
      遠藤 佑真(東北大学)、内田 健央、川又 生吹、鈴木 勇輝、野村 M. 慎一郎、村田 智
    • Organizer
      第1回分子ロボティクス年次大会(東北大学)
    • Related Report
      2018 Research-status Report
  • [Presentation] DNAナノ構造形成における微小空間の効果に関する研究2018

    • Author(s)
      渡辺泰基(東北大学)、村田智、野村慎一郎、川又生吹、鈴木勇輝
    • Organizer
      第1回分子ロボティクス年次大会(東北大学)
    • Related Report
      2018 Research-status Report
  • [Presentation] 複数の自己集合モードを持つDNAオリガミ構造体の設計と構築2018

    • Author(s)
      武田祐貴(東北大学)、鈴木勇輝、川又生吹、野村慎一郎、村田智
    • Organizer
      第1回分子ロボティクス年次大会(東北大学)
    • Related Report
      2018 Research-status Report
  • [Book] 分子ロボティクス概論~分子のデザインでシステムをつくる2019

    • Author(s)
      分子ロボティクス研究会著,村田智編
    • Total Pages
      302
    • Publisher
      CBI学会出版
    • ISBN
      9784990907648
    • Related Report
      2018 Research-status Report

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Published: 2018-07-25   Modified: 2021-02-19  

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