Designing lipid membrane nanodomains using DNA origami
| Project/Area Number |
18K19831
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 62:Applied informatics and related fields
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| Research Institution | Tohoku University |
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Principal Investigator |
Suzuki Yuki 東北大学, 学際科学フロンティア研究所, 助教 (50636066)
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| Project Period (FY) |
2018-06-29 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | DNAナノテクノロジー / DNAオリガミ / 脂質膜 / 原子間力顕微鏡 / 自己集合化 / 自己組織化 / 膜ドメイン / 分子デザイン / 相分離 |
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| Outline of Final Research Achievements |
The fluidity of the plasma membrane not only allows the diffusion of proteins and other biomolecules within the membrane but also affects the function of these molecules through the formation of membrane nanodomains. Techniques for artificially producing lipid membrane nanodomains would enable us to reconstitute biological phenomena take place on the cellular membranes. In this study, we aimed to develop a technique to design artificial membrane nanodomains using DNA origami nanothechnology. DNA origami nanostructures were self-assembled into two-dimensional lattices on a mica-supported lipid bilayer membranes, which resulted in the compartmentalization of the bilayer surface into nanoscale domains with predesigned shapes which designated by the shapes of DNA origami building blocks.
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| Academic Significance and Societal Importance of the Research Achievements |
人工的に再構成した脂質二重膜は生体膜反応に関わる脂質やタンパク質の挙動を解析するためのモデルシステムとなるだけでなく，生体適合界面としても利用されており，その表面のプロセシング及び機能付加は新規バイオデバイス・バイオマテリアルの創成につながると期待される．DNAによって仕切られた脂質膜のナノドメインは，膜タンパク質をはじめとしたさまざまな分子をデザインした周期で配列化するために利用できるほか，それらの分子の空間的配置や局所的な密度を精密制御するための基盤技術としても応用が期待できる．
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Report
(4 results)
Research Products
(18 results)
