Development zeno-free/self-feeder culture system for an efficient iPSC derived platelet production
Project/Area Number |
18K19910
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 90:Biomedical engineering and related fields
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Research Institution | Nagoya University |
Principal Investigator |
Tamura Shogo 名古屋大学, 医学系研究科(保健), 講師 (60722626)
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Co-Investigator(Kenkyū-buntansha) |
小嶋 哲人 名古屋大学, 医学系研究科(保健), 教授 (40161913)
佐々木 知幸 山梨大学, 大学院総合研究部, 助教 (40739124)
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Project Period (FY) |
2018-06-29 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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Keywords | 巨核球 / 造血微小環境 / iPS細胞 / 骨髄微小環境 / 血小板 |
Outline of Final Research Achievements |
The aim of this study is to establish a high efficient in vitro culture system of megakaryocyte/platelet using a novel bone marrow podoplanin (PDPN) positive stromal cells which we previously identified. PDPN positive stromal cells obviously promoted an expansion of megakaryo-progenitor in vitro. However, in the co-culture system with PDPN positive stromal cells and megakaryocytes, we found that platelet collection rate was significantly reduced because of unexpected platelet adhesion onto stromal cells.
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Academic Significance and Societal Importance of the Research Achievements |
in vitorにおける巨核球・血小板培養効率を向上させるためには骨髄の造血環境の再構築が必須である。本研究は我々が新たに発見したPDPN間葉系細胞をフィーダー細胞として用いたが、静置条件下での培養ではフィーダー細胞への血小板の接着が問題になることを明らかにした。今後は、非静置条件での培養システムや、血小板産生に関わる分子に着目したビーズなどの非フィーダー細胞系の血小板大量産生培養システムの開発が必要になることが示された。
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] Higher FVIII:C Measured by Chromogenic Substrate Assay Than by One-Stage Assay Is Associated With Silent Hemophilic Arthropathy2020
Author(s)
Mika Ogawa, Nobuaki Suzuki, Nobunori Takahashi, Shogo Tamura, Atsuo Suzuki, Sachiko Suzuki, Yuua Hattori, Misaki Kakihara, Takeshi Kanematsu, Toshihisa Kojima, Akira Katsumi, Fumihiko Hayakawa, Tetsuhito Kojima, Naoki Ishiguro , Hitoshi Kiyoi , Tadashi Matsushita
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Journal Title
Thromb Res
Volume: 188
Pages: 103-105
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Apparent Synonymous Mutation F9 c.87A>G Causes Secretion Failure by In-Frame Mutation With Aberrant Splicing2019
Author(s)
Odaira K, Tamura S, Suzuki N, Kakihara M, Hattori Y, Tokoro M, Suzuki S, Takagi A, Katsumi A, Hayakawa F, Okamoto S, Suzuki A, Kanematsu T, Matsushita T, Kojima T.
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Journal Title
Thromb Res
Volume: 179
Pages: 95-103
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] A Complex F8 Rearrangement Associated with Template Switching and int1h-related Homologous Recombination in a Patient with Severe Hemophilia A2019
Author(s)
M. Tokoro, S. Tamura, N. Suzuki, M. Kakihara, Y. Hattori, K. Odaira, S. Suzuki, A. Takagi, F. Hayakawa, S. Okamoto, T. Kanematsu, T. Matsushita, T. Kojima
Organizer
ISTH2019
Related Report
Int'l Joint Research
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[Presentation] F9 c.87A>G is a Double-faced Deleterious Mutation which Causes a Synonymous and Inframed-mutant FIX due to an Aberrant mRNA Splicing2019
Author(s)
K. Odaira, S. Tamura, N. Suzuki, M. Kakihara, Y. Hattori, M. Tokoro, S. Suzuki, A. Takagi, A. Katsumi, F. Hayakawa, S. Okamoto, A. Suzuki, T. Kanematsu, T. Matsushita, T. Kojima
Organizer
ISTH2019
Related Report
Int'l Joint Research
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[Presentation] An Inv22-like F8 Inverted Disruption in Severe Hemophilia a Brothers Possibly Occurring from Template Switching between Sister Chromatids2019
Author(s)
M. Kakihara, S. Tamura, M. Tokoro, K. Odaira, Y. Hattori, S. Suzuki, F. Hayakawa, M. Ogawa, T. Kanematsu, N. Suzuki, T. Matsushita, T. Kojima
Organizer
ISTH2019
Related Report
Int'l Joint Research
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[Presentation] Genetic Abnormalities of Japanese Patient with Symptomatic- and Asymptomatic-dysfibrinogenemia2019
Author(s)
Y. Hattori, S. Tamura, N. Suzuki, M. Kakihara, S. Suzuki, K. Odaira, M. Tokoro, F. Hayakawa, S. Okamoto, T. Kanematsu, T. Matsushita, T. Kojima
Organizer
ISTH2019
Related Report
Int'l Joint Research
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