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Identification of mechanical signal from intercalated discs

Research Project

Project/Area Number 18K19924
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 90:Biomedical engineering and related fields
Research InstitutionOkayama University

Principal Investigator

Katanosaka Yuki  岡山大学, 医歯薬学総合研究科, 講師 (60432639)

Co-Investigator(Kenkyū-buntansha) 片野坂 公明  中部大学, 生命健康科学部, 准教授 (50335006)
氏原 嘉洋  名古屋工業大学, 工学(系)研究科(研究院), 准教授 (80610021)
Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords心不全 / メカニカルストレス / 血行動態負荷 / 心筋細胞 / メカノセンサー / TRPV2 / トランスレーショナルリサーチ / 多階層 / 介在板 / Ca2+シグナル / 心臓 / 心肥大 / カルシウム / メカニカルシグナル
Outline of Final Research Achievements

The heart has a dynamic compensatory mechanism for haemodynamic stress. However, the molecular detail of cardiac mechano-transduction are unclear. We have previously proposed that TRP vanilloid family type 2 channel (TRPV2) serves as a mechanoreceptor at intercalated discs of cardiomyocytes. Here, we analyzed that the mechanical-stimulation-induced Ca2+ change at intercalated area of neonatal cardiomyocytes. These data show that TRPV2-dependent mechanical-stimulation-induced Ca2+ change is critical for the development of Ca2+-handling of beating myocytes during the maturation process of neonatal cardiomyocytes. In addition, we show that the TRPV2-dependent mechanical-stimulation-induced Ca2+ change is not observed in dystrophic myocytes.

Academic Significance and Societal Importance of the Research Achievements

心筋細胞は、血行動態変化(メカニカルストレス)に応じて形や機能を変容させる。この際には、心筋細胞のメカニカルストレス依存的Ca2+シグナルを利用することが知られているが、その分子基盤は不明のままである。また、心筋細胞では、拍動ごと(収縮期)に筋小胞体由来のCa2+が一過的に上昇するため、収縮期に上昇するCa2+と混同することなく、メカのセンサーを介したCa2+シグナルが有効に働くためには、細胞内での『Ca2+波の空間やタイミング』に特性があると考えられる。本研究は、新しい心不全治療ターゲット分子を提案するために、心筋細胞のメカノセンシング機構の分子基盤を得るものである。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (11 results)

All 2019 2018

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results)

  • [Journal Article] Elimination of fukutin reveals cellular and molecular pathomechanisms in muscular dystrophy-associated heart failure2019

    • Author(s)
      Ujihara Y, Kaangawa M, Mohri S, Takatsu S, Kobayashi K, Toda T, Naruse K, Katanosaka Y
    • Journal Title

      Nature Communications

      Volume: -

    • NAID

      120006777089

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] TRPV2を核とした心臓のメカノバイオロジー研究2019

    • Author(s)
      片野坂友紀
    • Journal Title

      医学のあゆみ

      Volume: 270 Pages: 904-909

    • Related Report
      2019 Annual Research Report
  • [Journal Article] TRPV2 is required for mechanical nociception and the stretch-evoked response of primary sensory neurons.2018

    • Author(s)
      Kimiaki Katanosaka, Satomi Takatsu, Kazue Mizumura, Keiji Naruse, Yuki Katanosaka
    • Journal Title

      Scientific Reports

      Volume: 8(1) Issue: 1 Pages: 16782-16791

    • DOI

      10.1038/s41598-018-35049-4

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 心臓のメカノバイオロジー ~心筋細胞のTRPV2の役割を中心に~2019

    • Author(s)
      片野坂友紀
    • Organizer
      第42回日本高血圧学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] The role of TRPV2 during the development of excitation-contraction coupling in neonatal cardiomyocytes2019

    • Author(s)
      Katanosaka Y, Ujihara Y, Katanosaka K, Naruse K
    • Organizer
      Gordon Conference muscle E-C coupling
    • Related Report
      2019 Annual Research Report
  • [Presentation] TRPV2 is crucial for the development of intercalated discs in mouse hearts.2019

    • Author(s)
      Katanosaka Y, Shibuya M, Ujihara Y, Mohri S, Naruse K
    • Organizer
      63nd Biophysical Society
    • Related Report
      2019 Annual Research Report
  • [Presentation] TRPV2 is crucial for the development of intercalated discs in mouse hearts.2019

    • Author(s)
      Yuki Katanosaka
    • Organizer
      63rd annual meeting of biophysical society
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 心臓の分化・成熟およびストレス応答におけるメカノセンサーTRPV2の役割2018

    • Author(s)
      片野坂友紀
    • Organizer
      第57回日本生体医工学会大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Fukutin-KO心筋細胞から探る筋ジストロフィー関連心筋症の病態発症メカニズム2018

    • Author(s)
      片野坂友紀
    • Organizer
      第4回 日本筋学会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 心臓の可塑性や生理機能を支える膜輸送体:メカノセンサーTRPV22018

    • Author(s)
      片野坂友紀
    • Organizer
      第91回 日本生化学会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 骨格筋特異的TRPV2ノックアウトマウスを用いたメカニカルストレス応答の解析2018

    • Author(s)
      澁谷慎、日比野雄平、片野坂公明、片野坂友紀
    • Organizer
      第6回 若手による骨格筋細胞研究会
    • Related Report
      2018 Research-status Report

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Published: 2018-07-25   Modified: 2021-02-19  

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