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In vivo imaging of inflammasome activation for clinical diagnosis

Research Project

Project/Area Number 18K19929
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 90:Biomedical engineering and related fields
Research InstitutionKyushu University

Principal Investigator

MURATA MASAHARU  九州大学, 先端医療イノベーションセンター, 特任教授 (30304744)

Co-Investigator(Kenkyū-buntansha) 梅野 太輔  千葉大学, 大学院工学研究院, 教授 (00400812)
赤星 朋比古  九州大学, 医学研究院, 准教授 (20336019)
橋爪 誠  九州大学, 先端医療イノベーションセンター, 名誉教授 (90198664)
河野 喬仁  九州大学, 先端医療イノベーションセンター, 特任助教 (90526831)
Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywordsナノ材料 / DDS / 分子イメージング / 診断システム / 炎症 / 画像診断 / ナノバイオメディスン / インフラマソーム / 診断
Outline of Final Research Achievements

We are developed novelnano-drug carrier modeled on the viral capsid. In this project, we designed and created a functionalized contrast agent that can react specifically with inflammasome and change the signal according to its activity. As its platform, we use protein-based bio-nanocapsules (small heat shock protein, HSP16.5) and incorporate the concepts of protein engineering and evolutionary engineering to respond to inflammasome more sensitively and quantitatively. Was newly designed. The obtained recombinant was a spherical structure with a diameter of 20 nm and responded to inflammasome in a concentration-dependent manner.

Academic Significance and Societal Importance of the Research Achievements

がん組織に炎症細胞が浸潤していることは、病理学的に古くから知られており、慢性炎症と発がんは密接に関わっていると考えられてきた。特に近年は炎症反応により活性化するシグナル経路が発がんに関与していることが解明されつつあり、炎症応答を制御することでがん化やその進展を制御できる可能性も見えてきた。また炎症性疾患には本研究の目標であるNASHをはじめ、機序、診断法、そして治療法が開発途上にあるものも多く、炎症シグナルの中核を成すインフラマソームの解明が喫緊の課題となっている。本研究で開発したインフラマソーム応答型ナノカプセルはその造影剤として機能することができる。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (10 results)

All 2020 2019 2018 2017

All Journal Article (10 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 10 results,  Open Access: 3 results)

  • [Journal Article] Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells2020

    • Author(s)
      Asai, D, Kawano, T, Murata, M, Nakashima, H, Toita, R, Kang, J.H
    • Journal Title

      Journal of Oleo Science

      Volume: 69 Issue: 3 Pages: 255-260

    • DOI

      10.5650/jos.ess19268

    • NAID

      130007804109

    • ISSN
      1345-8957, 1347-3352
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In vivo melanoma imaging based on dynamic nuclear polarization enhancement in melanin pigment of living mice using in vivo dynamic nuclear polarization magnetic resonance imaging2019

    • Author(s)
      Hyodo Fuminori、Naganuma Tatsuya、Eto Hinako、Murata Masaharu、Utsumi Hideo、Matsuo Masayuki
    • Journal Title

      Free Radical Biology and Medicine

      Volume: 134 Pages: 99-105

    • DOI

      10.1016/j.freeradbiomed.2019.01.002

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of lysophosphatidylcholine-induced human aortic smooth muscle cell calcification by protein kinase A inhibition2019

    • Author(s)
      Riki Toita, Daisuke Asai, Kentaro Otani, Takahito Kawano, Masaharu Murata, Jeong-Hun Kang
    • Journal Title

      Lipids

      Volume: 54 Issue: 8 Pages: 465-470

    • DOI

      10.1002/lipd.12178

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Advanced multispectral image-processing endoscopy system for visualizing two-dimensional hemoglobin saturation and relative hemoglobin concentration2019

    • Author(s)
      Chiba Toru、Obara Yoshimi、Murata Masaharu、Akahoshi Tomohiko
    • Journal Title

      Endoscopy International Open

      Volume: 07 Issue: 11 Pages: E1442-E1447

    • DOI

      10.1055/a-0990-9189

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phase Separation Multi-phase Flow Using an Aqueous Two-phase System of a Polyethylene Glycol/Dextran Mixed Solution2018

    • Author(s)
      Naoya IMANISHI, Tetsuo YAMASAKI, and Kazuhiko TSUKAGOSHI, Masaharu MURATA
    • Journal Title

      Analytical Sciences

      Volume: 34 Issue: 8 Pages: 953-958

    • DOI

      10.2116/analsci.18P105

    • NAID

      130007432308

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2018-08-10
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ligand-Mediated Coating of Liposomes with Human Serum Albumin2018

    • Author(s)
      Sato Hikari、Nakhaei Elnaz、Kawano Takahito、Murata Masaharu、Kishimura Akihiro、Mori Takeshi、Katayama Yoshiki
    • Journal Title

      Langmuir

      Volume: 34 Issue: 6 Pages: 2324-2331

    • DOI

      10.1021/acs.langmuir.7b04024

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Protein kinase A (PKA) inhibition reduces human aortic smooth muscle cell calcification stimulated by inflammatory response and inorganic phosphate2018

    • Author(s)
      Riki Toita, Kentaro Otani, Takahito Kawano, Satoshi Fujita, Masaharu Murata, Jeong-Hun Kang
    • Journal Title

      Life Sciences

      Volume: 209 Pages: 466-471

    • DOI

      10.1016/j.lfs.2018.08.051

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ultrasensitive MRI detection of spontaneous pancreatic tumors with nanocage-based targeted contrast agent.2018

    • Author(s)
      Kawano T, Murata M, Kang JH, Piao JS, Narahara S, Hyodo F, Hamano N, Guo J, Oguri S, Ohuchida K, Hashizume M
    • Journal Title

      Biomaterials

      Volume: 152 Pages: 37-46

    • DOI

      10.1016/j.biomaterials.2017.10.029

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Increased hepatic inflammation in a normal-weight mouse after long-term high-fat diet feeding2017

    • Author(s)
      Riki TOITA, Takahito KAWANO, Satoshi FUJITA, Masaharu MURATA, Jeong-Hun KANG
    • Journal Title

      Journal of Toxicologic Pathology

      Volume: 31 Issue: 1 Pages: 43-47

    • DOI

      10.1293/tox.2017-0038

    • NAID

      130006318210

    • ISSN
      0914-9198, 1881-915X
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Postmortem interval estimation using the animal model of postmortem gas volume changes.2017

    • Author(s)
      Iwamoto C, Ohuchida K, Okumura M, Usumoto Y, Kishimoto J, Murata M, Ikeda N, Hashizume M.
    • Journal Title

      Legal Medicine

      Volume: - Pages: 66-70

    • DOI

      10.1016/j.legalmed.2017.12.010

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access

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Published: 2018-07-25   Modified: 2021-02-19  

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