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Development of three-dimensional brain tissue engineering by the combination of angiogenesis and neurogenesis models

Research Project

Project/Area Number 18K19937
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 90:Biomedical engineering and related fields
Research InstitutionKeio University

Principal Investigator

Sudo Ryo  慶應義塾大学, 理工学部(矢上), 准教授 (20407141)

Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords三次元培養 / 脳 / 血管新生 / 神経新生
Outline of Final Research Achievements

The purpose of this study is to construct a culture model of neurovascular unit, which is a three-dimensional (3D) unit structure in the brain, by 3D culture in a microfluidic device from the standpoint of tissue engineering. This study was conducted by focusing on the development of an angiogenesis model, which was followed by the combination of the angiogenesis model and a neurogenesis model. First, we constructed a microfluidic device, which is a common culture platform for both angiogenesis and neurogenesis models. We then investigated an angiogenesis model which is useful in brain tissue engineering. Furthermore, we constructed a brain angiogenesis model by recapitulating brain developmental stages. Finally, we successfully constructed a 3D NVU culture model by combining angiogenesis and neurogenesis models.

Academic Significance and Societal Importance of the Research Achievements

生体外で脳組織を構築しようとする学術基盤において欠如している三次元脳組織工学を開拓することによって、【神経科学(単一細胞レベル)→三次元脳組織工学(多細胞レベル)→脳オルガノイド培養(組織レベル)】のシームレスな脳組織工学の学問体系を創出し、再生医療・創薬研究への応用に発展させる点に学術的意義がある。また、脳組織工学は、脳機能を解明するための実験モデルとしての役割だけでなく、病気のメカニズムや創薬研究、診断デバイス開発においても重要な位置づけとなる点で社会的意義がある。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (18 results)

All 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (10 results) (of which Int'l Joint Research: 6 results,  Invited: 3 results) Book (1 results) Remarks (1 results)

  • [Int'l Joint Research] Harvard University(米国)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Multi-cellular Morphogenesis Derived by Interstitial Flow2020

    • Author(s)
      須藤 亮
    • Journal Title

      Seibutsu Butsuri

      Volume: 60 Issue: 1 Pages: 025-030

    • DOI

      10.2142/biophys.60.025

    • NAID

      130007790187

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of Continuous Capillary Networks Stabilized by Pericyte-like Perivascular Cells2019

    • Author(s)
      Kyoko Yamamoto, Kohei Tanimura, Masafumi Watanabe, Hiromu Sano, Hiroyuki Uwamori, Yo Mabuchi, Yumi Matsuzaki, Seok Chung, Roger D. Kamm, Kazuo Tanishita, Ryo Sudo
    • Journal Title

      Tissue Engineering Part A

      Volume: 25 Issue: 5-6 Pages: 499-510

    • DOI

      10.1089/ten.tea.2018.0186

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Balance of interstitial flow magnitude and vascular endothelial growth factor concentration modulates three-dimensional microvascular network formation2019

    • Author(s)
      Abe Yoshinori、Watanabe Masafumi、Chung Seok、Kamm Roger D.、Tanishita Kazuo、Sudo Ryo
    • Journal Title

      APL Bioengineering

      Volume: 3 Issue: 3

    • DOI

      10.1063/1.5094735

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Establishment of an <i>in vitro</i> vascular anastomosis model in a microfluidic device2019

    • Author(s)
      Masafumi Watanabe, Ryo Sudo
    • Journal Title

      Journal of Biomechanical Science and Engineering

      Volume: 14 Issue: 3 Pages: 18-00521-18-00521

    • DOI

      10.1299/jbse.18-00521

    • NAID

      130007741719

    • ISSN
      1880-9863
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparison of organ-specific endothelial cells in terms of microvascular formation and endothelial barrier functions2019

    • Author(s)
      Uwamori Hiroyuki、Ono Yuuichi、Yamashita Tadahiro、Arai Ken、Sudo Ryo
    • Journal Title

      Microvascular Research

      Volume: 122 Pages: 60-70

    • DOI

      10.1016/j.mvr.2018.11.007

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Engineering of in vitro vascular anastomosis under vascular endothelial growth factor (VEGF) gradients in a microfluidic device2019

    • Author(s)
      Masafumi Watanabe, Ryo Sudo
    • Organizer
      TERMIS (Tissue Engineering and Regenerative Medicine International Society)-EU 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In vitro 3D tissue engineering using a microfluidic device2019

    • Author(s)
      Ryo Sudo
    • Organizer
      Nano LSI Open Seminar
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Microfluidic systems for 3D tissue engineering of cancer and vascular tissues2019

    • Author(s)
      Ryo Sudo
    • Organizer
      Microfluidics & Organ-on-a-Chip Asia 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] メソスケールデバイスを用いた灌流による毛細血管網の形態変化の調査2019

    • Author(s)
      大野航平、菅野隼、山下忠紘、須藤亮
    • Organizer
      日本機械学会第32回バイオエンジニアリング講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ヒト神経幹細胞由来アストロサイトと血管新生モデルの融合による血液脳関門モデルの開発2019

    • Author(s)
      木保和也、柴隆太、山下忠紘、須藤亮
    • Organizer
      日本機械学会第32回バイオエンジニアリング講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] In vitro tissue engineering using 3D microfluidic devices2019

    • Author(s)
      Ryo Sudo
    • Organizer
      International Symposium on SSS Laser Processing
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 3D brain angiogenesis platform on a microfluidic device2019

    • Author(s)
      Kazuya Kibo, Takuya Higuchi, Mizuki Ichikawa, Hiroyuki Uwamori, Tadahiro Yamashita, Ryo Sudo
    • Organizer
      3rd International Symposium on Nanoarchitectonics for Mechanobiology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Investigation of a 3D Brain Angiogenesis Model Mimicking CNS Developmental Stages2018

    • Author(s)
      Kazuya Kibo, Takuya Higuchi, Mizuki Ichikawa, Hiroyuki Uwamori, Ryo Sudo
    • Organizer
      5th TERMIS (Tissue Engineering and Regenerative Medicine International Society) World Congress
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Creating a 3D brain angiogenesis model with MSCs and neural cells mimicking CNS developmental stages2018

    • Author(s)
      Kazuya Kibo, Takuya Higuchi, Mizuki Ichikawa, Hiroyuki Uwamori, Ryo Sudo
    • Organizer
      EMBS Micro and Nanotechnology in Medicine Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 灌流可能な脳微小血管網を用いた三次元血液脳関門モデルの構築2018

    • Author(s)
      市川瑞紀、須藤亮
    • Organizer
      日本機械学会 第31回バイオエンジニアリング講演会
    • Related Report
      2018 Research-status Report
  • [Book] 臓器チップの技術と開発動向(第7 章  マイクロ流体システムによる血管形成モデルと肝細胞3 次元培養モデルの融合)2018

    • Author(s)
      酒井康行、金森敏幸 監修 (須藤 亮 分担執筆)
    • Publisher
      シーエムシー出版
    • Related Report
      2018 Research-status Report
  • [Remarks] 慶應義塾大学理工学部システムデザイン工学科須藤研究室ホームページ

    • URL

      http://www.sudo.sd.keio.ac.jp

    • Related Report
      2019 Annual Research Report 2018 Research-status Report

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Published: 2018-07-25   Modified: 2021-02-19  

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