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A gene delivery microdevice for single cells

Research Project

Project/Area Number 18K19942
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 90:Biomedical engineering and related fields
Research InstitutionMeijo University

Principal Investigator

Kumagai Shinya  名城大学, 理工学部, 教授 (70333888)

Co-Investigator(Kenkyū-buntansha) 小林 未明  奈良先端科学技術大学院大学, 先端科学技術研究科, 博士研究員 (60393807)
Project Period (FY) 2018-06-29 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsプラズマオンチップ / マイクロデバイス / 細胞 / 遺伝子導入 / 大気圧プラズマ / シングルセル / プラズマ / 細胞分化制御 / 形質転換 / 幹細胞
Outline of Final Research Achievements

Transfection is the process of inserting genetic material, such as DNA into cells. The method is a powerful tool to control cell trait. However, it has been difficult to induce gene expression equally in all cells treated. In this study, we used a plasma, which is the fourth state of matter, for effective transfection. Utilizing micro electromechanical systems (MEMS), a microwell array device was fabricated that enables plasma treatment on single cells cultured in each microwell. Introduction of DNA and chemical substances into cells were achieved by plasma treatment. The microsystem is an important first step towards controllable transfection that has a potential to revolutionize protocol for establishing iPS cells.

Academic Significance and Societal Importance of the Research Achievements

本研究は、プラズマ照射型シングルセル遺伝子導入デバイスの開発である。一細胞に対して確実に遺伝子導入を行って、その細胞をマイクロウェル構造の中で増殖させることで、同じ遺伝情報を持つ細胞群(クローン)を高い効率で作り出す。将来性の一つとして、iPS細胞の樹立には山中4因子を導入する必要があり、樹立確立は1%程度と低いが、本手法で各因子を細胞内に確実に導入し、高効率に初期化できる可能性がある。本研究は、医工学関連分野でのイノベーションを起こす第一歩となる意義を持っている。

Report

(3 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • Research Products

    (17 results)

All 2020 2019 2018 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 7 results,  Invited: 3 results) Remarks (6 results)

  • [Journal Article] Measurement of hydroxyl radicals delivered into liquid through a micro gas-liquid interface2020

    • Author(s)
      Koichi Ishikawa, Minoru Sasaki, Shinya Kumagai
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 59 Issue: SA Pages: SAAC11-SAAC11

    • DOI

      10.7567/1347-4065/ab5171

    • NAID

      210000157438

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Measurement of time evolution of fluorescent reagent introduction rate into cells towards efficient plasma gen transfection2020

    • Author(s)
      Tatsuya Kitazaki, Shinya Kumagai
    • Organizer
      ISPlasma2020/IC-PLANTS2020
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of non-thermal atmospheric pressure plasma on iPS cell differentiation2020

    • Author(s)
      Mime Kobayashi, Kiichiro Tomoda, Michio Asahi, Shinya Kumagai
    • Organizer
      The 67th JSAP Spring Meeting 2020
    • Related Report
      2019 Annual Research Report
  • [Presentation] Plasma-on-Chip for Biological Application: A Microdevice for Stimulating Single Cell with Non-Thermal Atmospheric Pressure Plasma2019

    • Author(s)
      Shinya Kumagai
    • Organizer
      2019 International Conference on Solid State Devices and Materials, Satellite Workshop
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Irradiating single cells with cold atmospheric pressure plasma using a microdevice2019

    • Author(s)
      Shinya Kumagai, Mime Kobayashi, Tetsuji Shimizu, Minoru Sasaki
    • Organizer
      Asian-Europian International Conference on Plasma Surface Engineering (AEPSE 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Plasma-on-Chip: A micro device for irradiating single cells with non-thermal atmospheric pressure plasma2019

    • Author(s)
      Shinya Kumagai, Mime Kobayashi, Jun-Seok Oh, Tetsuji Shimizu, Minoru Sasak
    • Organizer
      3rd Asia-Pacific Conference on Plasma Physics (AAPPS-DPP 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Improving substance intake efficiency using non-thermal atmospheric pressure plasma towards single cell gene transfection2019

    • Author(s)
      Atsuki Hobo, Mime Kobayashi, Shinya Kumagai
    • Organizer
      The 80 th JSAP Autumn Meeting 2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] Low temperature plasma for controlling iPS cell differentiation2019

    • Author(s)
      Mime Kobayashi, Kiichiro Tomoda, Michio Asahi, Shinya Kumagai
    • Organizer
      The 72nd Annual Gaseous Electronics Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Plasma-on-Chip: An innocative microdevice targeting for cell fate control using non-thermal atmospheric pressure plasma2019

    • Author(s)
      Shinya Kumagai, Mime Kobayashi, Tetsuji Shimizu, Minoru Sasaki
    • Organizer
      CiRA 2019 International Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of non-thermal atmospheric pressure plasma on iPS cell differentiation2019

    • Author(s)
      Mime Kobayashi, Kiichiro Tomoda, Michio Asahi, Shinya Kumagai
    • Organizer
      CiRA 2019 International Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 【2018年度応用物理学会優秀論文賞受賞記念講演】Development of plasma-on-chip: Plasma treatment for individual cells cultured in media2018

    • Author(s)
      Shinya Kumagai, Chun-Yao Chang, Jonghyeon Jeong, Mime Kobayashi, Tetsuji Shimizu, Minoru Sasaki
    • Organizer
      2018年 第79回 応用物理学会秋季学術講演会
    • Related Report
      2018 Research-status Report
  • [Remarks] 名城大学 理工学部電気電子工学科 熊谷研究室

    • URL

      http://www1.meijo-u.ac.jp/~skumagai/cms_new/

    • Related Report
      2019 Annual Research Report
  • [Remarks] researchmap 熊谷慎也

    • URL

      https://researchmap.jp/sk1

    • Related Report
      2019 Annual Research Report
  • [Remarks] ORCID iD Shinya Kumagai

    • URL

      https://orcid.org/0000-0002-8701-9497

    • Related Report
      2019 Annual Research Report
  • [Remarks] 名城大学 理工学部 電気電子工学科 熊谷研究室

    • URL

      http://www1.meijo-u.ac.jp/~skumagai/cms_new/

    • Related Report
      2018 Research-status Report
  • [Remarks] researchmapにおける研究代表者の情報

    • URL

      https://researchmap.jp/sk1/

    • Related Report
      2018 Research-status Report
  • [Remarks] researchmapにおける研究分担者の情報

    • URL

      https://researchmap.jp/Las-e/?lang=japanese

    • Related Report
      2018 Research-status Report

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Published: 2018-07-25   Modified: 2021-02-19  

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