Elucidation of physical properties of biomolecules in the designed nanoscale space
| Project/Area Number |
18KK0139
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| Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 28:Nano/micro science and related fields
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| Research Institution | Kyoto University |
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Principal Investigator |
Endo Masayuki 京都大学, 理学研究科, 特定准教授 (70335389)
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| Co-Investigator(Kenkyū-buntansha) |
多田隈 尚史 東京大学, 定量生命科学研究所, 協力研究員 (10339707)
鈴木 勇輝 東北大学, 学際科学フロンティア研究所, 助教 (50636066)
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| Project Period (FY) |
2018-10-09 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2020: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2019: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Keywords | DNAナノテクノロジー / DNAオリガミ / 光ピンセット / 高速原子間力顕微鏡 / ナノ空間 / ナノ構造 / 酵素反応 / 転写反応 / グアニン四重鎖 / i-モチーフ |
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| Outline of Final Research Achievements |
In this research, we intend to elucidate the influence of nanoscale space on biomolecules and the interaction between space and molecules by measuring the physical properties of biomolecules and controlling their reactions in the nanospace. By arranging guanine quadruplex and i-motif in the nanospace created by DNA origami, we clarified that these DNA structures were greatly stabilized, which was derived from the state of water molecules in the nanospace. We also clarified that double-stranded DNA was destabilized in the nanospace, and that chemical modification of space affected the stability of molecules. We also successfully created a system that controls biomolecular reactions in nanospace and mechanically controlling DNA origami structures. By understanding the physical properties of the nanospace, these provide us great insights for controlling biomolecules.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、生体分子や構造に見られるナノスケールの空間が分子に与える影響と空間-分子間に働く相互作用の解明を目指した。空間を自由に設計できる技術と生体分子の安定性を一分子で計測する技術を組み合わせることで、これらの現象の一端を解明した。生体中は均一ではなく、構成される空間に偏りがあるため、これらの空間を使って反応の促進や減速を局所的に行っていることも考えられる。これは新たな知見であり、空間という環境要因によっても反応が制御できることを示している。本研究のナノ空間の物性の理解によって、空間に分子を配列することで生体分子反応を制御するなど新たな応用もできる。
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Report
(4 results)
Research Products
(97 results)
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[Journal Article] DNA Nanostructures for Targeted Antimicrobial Delivery2020
Author(s)
Mela Ioanna、Vallejo‐Ramirez Pedro P.、Makarchuk Stanislaw、Christie Graham、Bailey David、Henderson Robert M.、Sugiyama Hiroshi、Endo Masayuki、Kaminski Clemens F.
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Journal Title
Angewandte Chemie International Edition
Volume: 59
Issue: 31
Pages: 12698-12702
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Construction of integrated gene logic-chip2018
Author(s)
Takeya Masubuchi, Masayuki Endo, Ryo Iizuka, Ayaka Iguchi, Dong Hyun Yoon, Tetsushi Sekiguchi, Hao Qi, Ryosuke Iinuma, Yuya Miyazono, Shuichi Shoji, Takashi Funatsu, Hiroshi Sugiyama, Yoshie Harada, Takuya Ueda, Hisashi Tadakuma
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Journal Title
Nat. Nanotechnol.
Volume: 13
Issue: 10
Pages: 933-940
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Oscillatory movement of a dynein-microtubule complex crosslinked with DNA-origami2019
Author(s)
Shimaa A. Abdellatef, Hisashi Tadakuma, Yuichi Kondo, Kangmin Yan, Rofia Boudria, Kodai Fukumoto, Takashi Fujiwara, Hideo Higuchi, and Keiko Hirose
Organizer
64th Annual Meeting of Biophysical Society, San Diego Convention Center (San Diego, CA)
Related Report
Int'l Joint Research
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[Presentation] Construction of DNA origami base gene transcription nano chip2019
Author(s)
Masubuchi T, Endo M, Iizuka R, Iguchi A, Hyun YD, Sekiguchi T, Qi H, Iinuma R, Miyazono Y, Shoji S, Funatsu T, Sugiyama H, Harada Y, Ueda T, Tadakuma H
Organizer
第2回分子ロボティクス年次大会
Related Report
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[Presentation] Integrated gene logic-chip functioning in an artificial cell2018
Author(s)
Masubuchi T, Endo M, Iizuka R, Iguchi A, Yoon DH, Sekiguchi T, Qi H, Iinuma R, Miyazono Y, Shoji S, Funatsu T, Sugiyama H, Harada Y, Ueda T and Tadakuma H.
Organizer
The 2nd Joint Australia-Japan/Japan-Australia Joint RNA meeting
Related Report
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[Presentation] Integrated gene logic-chip functioning in an artificial cell2018
Author(s)
Masubuchi T, Endo M, Iizuka R, Iguchi A, Yoon DH, Sekiguchi T, Qi H, Iinuma R, Miyazono Y, Shoji S, Funatsu T, Sugiyama H, Harada Y, Ueda T and Tadakuma H.
Organizer
CBI学会2018年大会
Related Report
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[Book] 核酸科学ハンドブック2020
Author(s)
日本核酸化学会、杉本 直己
Total Pages
576
Publisher
講談社
ISBN
9784065207864
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