• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

High-Performance Catalyst and Device Prepared by Metalloporphyrin

Research Project

Project/Area Number 18KK0156
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 34:Inorganic/coordination chemistry, analytical chemistry, and related fields
Research InstitutionOsaka University

Principal Investigator

Hayashi Takashi  大阪大学, 大学院工学研究科, 教授 (20222226)

Co-Investigator(Kenkyū-buntansha) 大洞 光司  大阪大学, 大学院工学研究科, 准教授 (10631202)
吉川 佳広  国立研究開発法人産業技術総合研究所, エレクトロニクス・製造領域, 主任研究員 (30373294)
北岸 宏亮  同志社大学, 理工学部, 教授 (60448090)
Project Period (FY) 2018-10-09 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2020: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2019: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords超分子化学 / ポルフィリン / シクロデキストリン / ヘムタンパク質 / 金属ポルフィリン / 自己集積化
Outline of Final Research Achievements

In this study, we focused on iron porphyrins (heme molecules), cofactors in heme proteins, that activate and transport dioxygen and are responsible for various catalytic reactions. The research groups in Japan and France have combined their respective molecular design, synthesis, and characterization techniques to create catalysts and devices based on metalloporphyrins, analogues of this cofactor. In particular, with an awareness of the coordination sphere provided by proteins, we collaborated to create model molecules for heme proteins by taking advantage of unique chemical modifications of porphyrins and cyclodextrins. For example, strapped metal porphyrins, strapped porphyrin rotaxanes and metal porphyrin-cyclodextrin conjugates were prepared. Furthermore, we developed energy and electron transfer devices based on the integration of metalloporphyrins and heme proteins.

Academic Significance and Societal Importance of the Research Achievements

ヘムタンパク質のモデル研究はこれまで数多く報告されているが、その多くは金属ポルフィリンそのものの反応を追跡したり、有機溶媒の中で活性を評価する例が大半であった。本研究では、水中で働くモデルを作成することに加え、ポルフィリン分子の近傍にタンパク質の配位圏を模した分子設計を施し、より精緻なモデルを構築することに学術的意義が認められた。その結果、新しい酸素結合分子や、チトクロムcオキダーゼのモデル分子などを提案し、いくつかの国際共著論文として報告した。さらに、光合成の光捕集系を意識したポルフィリンやヘムタンパク質のユニークな集積体の構築も成果につながった。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (37 results)

All 2023 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (4 results) Journal Article (20 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 18 results,  Open Access: 5 results) Presentation (12 results) (of which Int'l Joint Research: 9 results,  Invited: 10 results) Remarks (1 results)

  • [Int'l Joint Research] ストラスブール大学(フランス)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] University of Strasbourg/University of Rennes 1(フランス)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] ストラスブール大学/レンヌ第一大学(フランス)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] CNRS/ストラスブール大学/レンヌ第一大学(フランス)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Disulfide Bond-mediated Oligomerization of a Green Fluorescent Protein in Solution2023

    • Author(s)
      Soon Julian Wong、Oohora Koji、Uchihashi Takayuki、Hayashi Takashi
    • Journal Title

      Chemistry Letters

      Volume: 52 Issue: 2 Pages: 105-109

    • DOI

      10.1246/cl.220495

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A disulphide bond-mediated hetero-dimer of a hemoprotein and a fluorescent protein exhibiting efficient energy transfer2022

    • Author(s)
      Soon Julian Wong、Oohora Koji、Hayashi Takashi
    • Journal Title

      RSC Advances

      Volume: 12 Issue: 44 Pages: 28519-28524

    • DOI

      10.1039/d2ra05249k

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Focusing on a nickel hydrocorphinoid in a protein matrix: methane generation by methyl-coenzyme M reductase with F430 cofactor and its models2022

    • Author(s)
      Miyazaki Yuta、Oohora Koji、Hayashi Takashi
    • Journal Title

      Chemical Society Reviews

      Volume: 51 Issue: 5 Pages: 1629-1639

    • DOI

      10.1039/d1cs00840d

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional Myoglobin Model Composed of a Strapped Porphyrin/Cyclodextrin Supramolecular Complex with an Overhanging COOH That Increases O<sub>2</sub>/CO Binding Selectivity in Aqueous Solution2021

    • Author(s)
      Mao Qiyue、Das Pradip K.、Le Gac St?phane、Boitrel Bernard、Dorcet Vincent、Oohora Koji、Hayashi Takashi、Kitagishi Hiroaki
    • Journal Title

      Inorganic Chemistry

      Volume: 60 Issue: 16 Pages: 12392-12404

    • DOI

      10.1021/acs.inorgchem.1c01628

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Myoglobins engineered with artificial cofactors serve as artificial metalloenzymes and models of natural enzymes2021

    • Author(s)
      Oohora Koji、Hayashi Takashi
    • Journal Title

      Dalton Transactions

      Volume: 50 Issue: 6 Pages: 1940-1949

    • DOI

      10.1039/d0dt03597a

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Supramolecular Assembly of Hemoproteins Formed in a Star-Shaped Structure via Heme?Heme Pocket Interactions2021

    • Author(s)
      Soon Julian Wong、Oohora Koji、Hirayama Shota、Hayashi Takashi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 3 Pages: 1012-1012

    • DOI

      10.3390/ijms22031012

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sensitive quantification of carbon monoxide in vivo?reveals a protective role of circulating hemoglobin in CO?intoxication2021

    • Author(s)
      Mao Qiyue、Kawaguchi Akira T.、Mizobata Shun、Motterlini Roberto、Foresti Roberta、Kitagishi Hiroaki
    • Journal Title

      Communications Biology

      Volume: 4 Issue: 1 Pages: 425-425

    • DOI

      10.1038/s42003-021-01880-1

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Synthetic heme protein models that function in aqueous solution2021

    • Author(s)
      Kitagishi Hiroaki、Kano Koji
    • Journal Title

      Chemical Communications

      Volume: 57 Issue: 2 Pages: 148-173

    • DOI

      10.1039/d0cc07044k

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dynamic host-guest behavior in halogen-bonded two-dimensional molecular networks investigated by scanning tunneling microscopy at the solid/liquid interface2020

    • Author(s)
      Kikkawa Yoshihiro、Nagasaki Mayumi、Koyama Emiko、Tsuzuki Seiji、Fouquet Thierry、Hiratani Kazuhisa
    • Journal Title

      Nanoscale Advances

      Volume: 2 Issue: 10 Pages: 4895-4901

    • DOI

      10.1039/d0na00616e

    • Related Report
      2020 Research-status Report
  • [Journal Article] Construction of a Hexameric Hemoprotein Sheet and Direct Observation of Dynamic Processes of Its Formation2020

    • Author(s)
      Oohora Koji、Hirayama Shota、Uchihashi Takayuki、Hayashi Takashi
    • Journal Title

      Chemistry Letters

      Volume: 49 Issue: 2 Pages: 186-190

    • DOI

      10.1246/cl.190855

    • NAID

      130007795242

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Thermoresponsive Micellar Assembly Constructed from a Hexameric Hemoprotein Modified with Poly(N-isopropylacrylamide) toward an Artificial Light-Harvesting System2020

    • Author(s)
      Hirayama Shota、Oohora Koji、Uchihashi Takayuki、Hayashi Takashi
    • Journal Title

      Journal of the American Chemical Society

      Volume: 142 Issue: 4 Pages: 1822-1831

    • DOI

      10.1021/jacs.9b10080

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] ポルフィリンワイヤーの創製と走査型プローブ顕微鏡による解析~ストラスブール大学との共同研究~2020

    • Author(s)
      吉川佳広
    • Journal Title

      日仏工業技術

      Volume: 65 Pages: 7-10

    • Related Report
      2019 Research-status Report
  • [Journal Article] Hydrogen bond directed molecular recognition in water in a strapped-porphyrin-cyclodextrin assembly2019

    • Author(s)
      Kitagishi Hiroaki、Ohara Kazuki、Shimoji Daiki、Vonesch Maxime、Weiss Jean、Wytko Jennifer A.
    • Journal Title

      Journal of Porphyrins and Phthalocyanines

      Volume: 23 Issue: 04n05 Pages: 569-574

    • DOI

      10.1142/s1088424619500494

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Modelling haemoproteins: porphyrins and cyclodextrins as sources of inspiration2019

    • Author(s)
      Vonesch Maxime、Wytko Jennifer A.、Kitagishi Hiroaki、Kano Koji、Weiss Jean
    • Journal Title

      Chemical Communications

      Volume: 55 Issue: 97 Pages: 14558-14565

    • DOI

      10.1039/c9cc07545c

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Electrochemical CO2 reduction by a cobalt bipyricorrole complex: decrease of an overpotential value derived from monoanionic ligand character of the porphyrinoid species2019

    • Author(s)
      Ogawa Ayumu、Oohora Koji、Gu Wenting、Hayashi Takashi
    • Journal Title

      Chemical Communications

      Volume: 55 Issue: 4 Pages: 493-496

    • DOI

      10.1039/c8cc08876d

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photoinduced electron transfer within supramolecular hemoprotein co-assemblies and heterodimers containing Fe and Zn porphyrins2019

    • Author(s)
      Kajihara Ryota、Oohora Koji、Hayashi Takashi
    • Journal Title

      Journal of Inorganic Biochemistry

      Volume: 193 Pages: 42-51

    • DOI

      10.1016/j.jinorgbio.2019.01.001

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Arginine Residues Provide a Multivalent Effect for Cellular Uptake of a Hemoprotein Assembly2019

    • Author(s)
      Oohora Koji、Kajihara Ryota、Jiromaru Misa、Kitagishi Hiroaki、Hayashi Takashi
    • Journal Title

      Chemistry Letters

      Volume: 48 Issue: 3 Pages: 295-298

    • DOI

      10.1246/cl.180897

    • NAID

      130007607775

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Light triggers molecular shuttling in rotaxanes: control over proximity and charge recombination2019

    • Author(s)
      Wolf Maximilian、Ogawa Ayumu、Bechtold Mareike、Vonesch Maxime、Wytko Jennifer A.、Oohora Koji、Campidelli St?phane、Hayashi Takashi、Guldi Dirk M.、Weiss Jean
    • Journal Title

      Chemical Science

      Volume: 10 Issue: 13 Pages: 3846-3853

    • DOI

      10.1039/c8sc05328f

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Hemoproteins Reconstituted with Artificial Metal Complexes as Biohybrid Catalysts2019

    • Author(s)
      Oohora Koji、Onoda Akira、Hayashi Takashi
    • Journal Title

      Accounts of Chemical Research

      Volume: 52 Issue: 4 Pages: 945-954

    • DOI

      10.1021/acs.accounts.8b00676

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synthesis and Characterization of meso-Substituted Cobalt Tetradehydrocorrin and Evaluation of Its Electrocatalytic Behavior Toward CO2 Reduction and H2 Evolution2018

    • Author(s)
      Ogawa Ayumu、Oohora Koji、Hayashi Takashi
    • Journal Title

      Inorganic Chemistry

      Volume: 57 Issue: 23 Pages: 14644-14652

    • DOI

      10.1021/acs.inorgchem.8b02333

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Catalysis in Myoglobins Reconstituted with Artificial Metalloporphyrinoids2022

    • Author(s)
      Takashi Hayashi, Koji Oohora
    • Organizer
      12th International Conference on Porphyrins and Phthalocyanines
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Conversion of Myoglobin into Artificial Metalloenzymes2022

    • Author(s)
      Takashi Hayashi, Koji Oohora, Shunsuke Kato
    • Organizer
      8th Asian Conference on Coordination Chemistry
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Artificial Metalloenzymes Formed by Incorporation of Synthetic Metal Cofactors into Apomyoglobin2022

    • Author(s)
      Takashi Hayashi, Koji Oohora
    • Organizer
      44th International Conference on Coordination Chemistry
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Artificial Metalloenzymes Consisting of Conjugates between Apohemoproteins and Metal Complexes2021

    • Author(s)
      Takashi Hayashi
    • Organizer
      European Federation of Biotechnology 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Reconstituted Hemoproteins with Ni Corrinoids toward Functional Models of Methane-producing Enzyme2021

    • Author(s)
      Takashi Hayashi
    • Organizer
      11th International Conference on Porphyrins and Phthalocyanines
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Engineering of Hemoproteins to Create Artificial Metalloenzymes2021

    • Author(s)
      Takashi Hayashi
    • Organizer
      electronic Biological InorganicChemistry (eBIC)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Conversion of myoglobin to an artificial metalloenzyme2021

    • Author(s)
      Takashi Hayashi
    • Organizer
      Pacifichem2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Supramolecular Hemoprotein Assemblies2019

    • Author(s)
      Takashi Hayashi, Tsuyoshi Mashima, Ryota Kajihara, Shota Hirayama, Koji Oohora
    • Organizer
      19th International Conference on Biological Inorganic Chemistry (ICBIC19)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Hemoprotein Modification Toward Artificial Biocatalyst Generation2019

    • Author(s)
      Takashi Hayashi, Akira Onoda, Koji Oohora
    • Organizer
      Artzymes2.0
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Supramolecular Assembly of Engineered Cytochrome b562 Forming a Thermodynamically Stable Ring-shaped Trimer2019

    • Author(s)
      Koji Oohora, Ryota Kajihara, Takashi Hayashi
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Construction of a Micellar Assembly of Hexameric Hemoprotein and Evaluation of its Photochemical Behavior2019

    • Author(s)
      Shota Hirayama, Koji Oohora, Takashi Hayashi
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 表面分子集積と走査型トンネル顕微鏡による解析2018

    • Author(s)
      吉川佳広
    • Organizer
      高分子学会東北支部講演会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Remarks] 大阪大学大学院工学研究科林研究室

    • URL

      http://www.chem.eng.osaka-u.ac.jp/~hayashiken/index.html

    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-10-12   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi